Prevalence of Pathogenic Vibrio anguillarumAmong Oreochromis niloticusFish Fingerlings Infected with Saprolegniasis Around Qarun Lake

Abstract

V IBRIO anguillarum causes severe economic losses in fish and shellfish species and poses public health concerns. This study aimed to provide some information about the prevalence and virulence determinants of V. anguillarum isolated from the farmed Oreochromisniloticus fish fingerlings nearby Qarun Lake, El-Fayoum Governorate, Egypt. PCRtargeting the rpoA (RNA polymerase alpha subunit)gene was done forconfirmation of Vibriospp. Moreover, V. anguillarum isolates were biochemically identified and confirmed by 16S rRNA gene sequence.V. anguillarum isolates produced caseinase, gelatinase, lipase, lecithinase, haemolysins, and possessed biofilm formation. The virulence-related genes;empA, vah1, vah3 and vah4, flaA,angM, and angRwere detected in all tested isolates. Therefore, proper identification of V.anguillarumis crucial to better understand the ecology and distribution patterns of such pathogen within Egyptian aquaculture. Moreover, the water quality in these farms near Qarun Lake was less supportive for larval rearing activities of O. niloticus fish.

Filed Studies on The most Prevailing Bacterial Diseases Affecting Some Cultured Marine Fishes Egypt

“…Aquaculture represents an important sector in the Egyptian economy providing essential food facing the growing demand for animal protein [1]. Global shortage in protein production of animal origin had made most governorates to modern trends to increase the production rate of it.…”

Efficacy of combined inactivated vaccines against Vibrio alginolyticus and Streptococcus agalactiae infections in Nile tilapia in Egypt

“…This disease is caused by members of the Vibrionaceae family, including Vibrio anguillarum, V. parahaemolyticus, V. ordalii, V. harveyi, and V. alginolyticus. From these species, V. alginolyticus has been previously identified from several finfish species, such as turbot (Scophthalmus maximus) (Austin et al 1993), Mugil capito (Khalil and Abdel-Latif 2013), cobia (Liu et al 2004;Rajan et al 2001), Tilapia zillii (El-Sayed et al 2019), African catfish (Clarias gariepinus) (Abdelsalam et al 2021), Sparus aurata and Dicentrarchus labrax (Ben Kahla-Nakbi et al 2009), and Nile tilapia (Oreochromis niloticus) (Younes et al 2016). Streptococcosis is another frequently encountered disease that affects farmed fish species and is caused by several streptococcal strains such as Streptococcus agalactiae, S. iniae, and S. dysgalactiae (Klesius et al 2008;Van Doan et al 2022).…”

Prevalence and molecular characteristics of antimicrobial resistance of Aeromonas hydrophila, Salmonella spp., Vibrio cholerae, fecal coliform, and Escherichia coli in hybrid red tilapia and cultured water

“…vulnificus has been commonly found in estuarine water and shellfish with the prevalence ranging from 13.6% to 15% (Cruz et al, 2016;Baker-Austin and Oliver, 2018;King et al, 2021). However, V. vulnificus could also be found in freshwater tilapia raised in Taiwan and Egypt, and the prevalence ranging from 1.7% to 12.5% (Chen et al, 2006;Younes et al, 2016). In the U.S., V. vulnificus infection in humans had a high fatality rate compared to other foodborne pathogens, and these bacteria were responsible for more than 95% of seafood-related deaths (Jones and Oliver, 2009).…”