Prevalence of resistance phenotypes and genotypes to macrolide, lincosamide and streptogramin antibiotics in Gram-positive cocci isolated in Tunisian Bone Marrow Transplant Center

Bouchami, Ons; Achour, Wafa; Hassen, Assia Ben

doi:10.1016/j.patbio.2009.03.010

Cited by 5 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the S. epidermidis strains studied, the most frequently identified gene in strains exhibiting both cMLS B and iMLS B phenotypes was ermC , which is consistent with previous reports ( Reyes et al 2007 , Gherardi et al 2009 , Coutinho et al. 2010 , Bouchami et al 2011 , Brzychczy-Wloch et al 2013 , Heb & Gallert 2014 ). Only a few S. epidermidis exhibiting cMLS B phenotypes possessed ermA and/or ermB.…”

Section: Discussionsupporting

confidence: 91%

“…Only a few S. epidermidis exhibiting cMLS B phenotypes possessed ermA and/or ermB. Similar data have been previously reported ( Bouchami et al 2011 , Teodoro et al 2012 , Szczuka et al 2016 ). Moreover, the presence of other erm genes (e.g., ermF) has been rarely detected in Staphylococcus spp ( Roberts 2004) .…”

Section: Discussionsupporting

confidence: 90%

“…Moreover, among CoNS, the type of erm gene also depends on the geographical region of their isolation. For example, ermC was previously detected in 50% of the strains exhibiting MLS B resistance in Great Britain, whereas it was detected 90% of those in Denmark ( Lim et al 2002 , Gatermann et al 2007 , Cetin et al 2010 , Bouchami et al 2011 ) and in Mexico, ermA was reported as predominant in S. epidermidis ( Castro-Alarcón et al 2011 ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

Juda

Chudzik-Rząd

Malm

2016

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-resistant S. epidermidis, together with the evaluation of genes promoting the following different types of MLSB resistance:ermA, ermB, ermC,msrA, mphC, and linA/A’. Susceptibility to spiramycin was also examined. Among 75 erythromycin-resistantS. epidermidis isolates, the most frequent phenotypes were macrolides and streptogramins B (MSB) and constitutive MLSB (cMLSB). Moreover, all strains with the cMLSB phenotype and the majority of inducible MLSB (iMLSB) isolates were resistant to spiramycin, whereas strains with the MSB phenotype were sensitive to this antibiotic. The D-shape zone of inhibition around the clindamycin disc near the spiramycin disc was found for some spiramycin-resistant strains with the iMLSB phenotype, suggesting an induction of resistance to clindamycin by this 16-membered macrolide. The most frequently isolated gene was ermC, irrespective of the MLSB resistance phenotype, whereas the most often noted gene combination wasermC, mphC, linA/A’. The results obtained showed that the genes responsible for different mechanisms of MLSB resistance in S. epidermidis generally coexist, often without the phenotypic expression of each of them.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

Juda

Chudzik-Rząd

Malm

2016

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…However, these studies were mostly limited to the most frequently isolated staphylococci i.e. S. aureus and S. epidermidis [13, 34, 35, 41–43] . Recently, also other staphylococcus species such as S. hominis , S. haemolyticus , S. warneri and S. simulans , have appeared as etiological factors of serious human infections.…”

Section: Discussionmentioning

confidence: 99%

Differences in distribution of MLS antibiotics resistance genes in clinical isolates of staphylococci belonging to species: S. epidermidis, S. hominis, S. haemolyticus, S. simulans and S. warneri

Szemraj

Czekaj

Kalisz³

et al. 2019

BMC Microbiol

View full text Add to dashboard Cite

Background Macrolides and lincosamides are two leading types of antibiotics commonly used in therapies. The study examines the differences in resistance to these antibiotics and their molecular bases in S. epidermidis as well as in rarely isolated species of coagulase-negative staphylococci such as S. hominis , S . haemolyticus , S. warneri and S. simulans . The isolates were tested for the presence of the erm (A), erm (B), erm (C), lnu (A), msr (A), msr (B), mph (C), ere (A) and ere (B) genes. Phenotypic resistance to methicillin and mecA presence were also determined. Results The MLS B resistance mechanism was phenotypically found in isolates of species included in the study. The most prevalent MLS B resistance mechanism was observed in S. hominis , S. haemolyticus and S. epidermidis isolates mainly of the MLS B resistance constitutive type. Macrolide, lincosamide and streptogramin B resistance genes were rarely detected in isolates individually. The erm (B), ere (A) and ere (B) genes were not found in any of the strains. The erm (A) gene was determined only in four strains of S. epidermidis and S. hominis while lnu (A) was seen in eight strains (mainly in S. hominis ). The erm (C) gene was present in most of S. epidermidis strains and predominant in S. hominis and S. simulans isolates. The examined species clearly differed between one another in the repertoire of accumulated genes. Conclusions The presence of genes encoding the MLS B resistance among CoNS strains demonstrates these genes’ widespread prevalence and accumulation in opportunistic pathogens that might become gene reservoir for bacteria with superior pathogenic potential.

show abstract

Prevalence of antibiotic resistance pathogens in online fresh-cut fruit from Chongqing, China and controlling Enterococcus faecalis by bacteriocin GF-15

Chen²,

et al. 2022

LWT

View full text Add to dashboard Cite

Prevalence of resistance phenotypes and genotypes to macrolide, lincosamide and streptogramin antibiotics in Gram-positive cocci isolated in Tunisian Bone Marrow Transplant Center

Cited by 5 publications

References 46 publications

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

Differences in distribution of MLS antibiotics resistance genes in clinical isolates of staphylococci belonging to species: S. epidermidis, S. hominis, S. haemolyticus, S. simulans and S. warneri

Prevalence of antibiotic resistance pathogens in online fresh-cut fruit from Chongqing, China and controlling Enterococcus faecalis by bacteriocin GF-15

Contact Info

Product

Resources

About