Prevalence of Staphylococcal Cassette Chromosome mec and Panton-Valentine Leukocidin Gene Amongst the Methicillin-resistant Staphylococcus aureus Strains Isolated From Fowl Meat

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is considered to be one of the most important causes of foodborne diseases. Objective: The current examination was performed to examine the distribution of staphylococcal cassette chromosome mec (SCCmec) and Panton-Valentine leukocidin (PVL) gene amongst the MRSA strains isolated from raw fowl meat samples. Materials and Methods: A total of 240 fowl meat samples were collected and cultured. MRSA strains were identified using cefoxitin and oxacillin susceptibility tests. DNA samples extracted from the MRSA strains were subjected to polymerase chain reaction (PCR) for detection of SCCmec and PVL gene. Results: Twenty-two out of 240 (9.16%) raw fowl meat samples were positive for S. aureus strains. Twelve out of 22 S. aureus strains (54.54%) were determined as MRSA strains. The incidence of MRSA strains in raw chicken, turkey, quail, and ostrich meat samples was 66.66%, 50%, 50%, and 33.33%, respectively. The incidence of SCCmec IVa, SCCmec IVd, and SCCmec V was 50%, 8.33% and 41.66%, respectively. The applied method failed to detect SCCmec types I, II, III, IVb, and IVc. The incidence of the PVL gene amongst the MRSA strains was 75%. Conclusion: The presence of SCCmec IV and SCCmec V and PVL gene revealed occurrence of community-associated MRSA (CA-MRSA) in fowl meat samples. Further studies are required to find additional epidemiological aspects of the MRSA strains in fowl meat samples.

Prevalence and Characteristics of Multidrug-Resistant Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) CC398 Isolated from Quails (Coturnix Coturnix Japonica) Slaughtered for Human Consumption

“…As far as we know, no other study has reported the prevalence and carriage of MRSA in quails at the slaughterhouse level. Very few studies have been carried out so far in quails, and they report the presence of MRSA strains in quail eggshells or meat [36][37][38]. Saadati et al (2019) sampled meat from 70 quails randomly collected from the shopping centers in Iran and found only two (2.86%) MRSA strains [38].…”

“…Very few studies have been carried out so far in quails, and they report the presence of MRSA strains in quail eggshells or meat [36][37][38]. Saadati et al (2019) sampled meat from 70 quails randomly collected from the shopping centers in Iran and found only two (2.86%) MRSA strains [38]. Poultry, and poultry meat in general, seem to frequently carry MRSA strains.…”

Staphylococcus aureus in poultry, with special emphasis on methicillin-resistant strain infection: A comprehensive review from one health perspective

“…Recent studies revealed the presence of MRSA in raw and frozen chicken meat in different countries worldwide, such as China [20], Bangladesh [89,90], Hong Kong [22], and Egypt [21,91]. Interestingly, MRSA strains were also isolated from quail meat [92][93][94]. Recently, Saadati et al [95] found that 2 of 70 (2.86%) quail samples were MRSA strains in shopping centers of Iran.…”