Prevalence of staphylococcal enterotoxins in<i>Staphylococcus pseudintermedius</i>isolates from dogs with pyoderma and healthy dogs

Tanabe, Taishi; Toyoguchi, Midori; Hirano, Fumitaka; Chiba, Mei; Onuma, Kenta; Sato, Hisaaki

doi:10.1111/1348-0421.12069

Cited by 18 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalence of the enterotoxinrelated gene sec canine (16.5%) was close to the 12.6% reported by Becker et al (2001) but lower than the 24% described by Yoon et al (2010) and other authors who confirmed its presence in all tested isolates (Futagawa-Saito et al 2004). The enterotoxin production rate was previously described to be significantly higher in isolates from diseased dogs in comparison with healthy dogs (Sasaki et al 2005), but not in all studies (Tanabe et al 2013). The role of S. pseudintermedius enterotoxin-related toxins in the pathogenesis of dog skin infections is still not fully clarified.…”

Section: Discussionmentioning

confidence: 90%

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Melter¹,

Švec²,

Tkadlec³

et al. 2017

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (sec canine ) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin. Keywords: Staphylococcus pseudintermedius; macrolide lincosamide-streptogramin B (MLS B ) resistance; genotyping; virulence genesStaphylococcus pseudintermedius, a pathogen that infects dogs, is comparable to Stapylococcus aureus in humans. This analogy fits not only the phenotypic characteristics (e.g. similar colony morphology, pigment, haemolysis), but is also supported by the striking similarity in ecology/epidemiology (colonisation or infection of the skin and skin adnexa, vertical/horizontal transmission), expression of homologous virulence factors (cell wall-anchored proteins such as microbial surface components recognising adhesive matrix molecules; protein A; and enzymes, e.g. coagulase; secreted toxins, such as cytotoxins, exfoliative toxins, or superantigens) and pathogenicity (skin infections or invasive infections). These two coagulase-positive species probably evolved separately through adaptation

show abstract

Section: Discussionmentioning

confidence: 90%

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Melter¹,

Švec²,

Tkadlec³

et al. 2017

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…The role of S. pseudintermedius enterotoxins (SEs) in the pathogenesis of pyoderma has been investigated (Tanabe et al 2013) and no differences between healthy and diseased dogs were detected, suggesting that these toxins do not participate in the pathogenesis of this disease. The gene sec was the only one found in S. pseudintermedius strains isolated from carriers and infected dogs and at a very low prevalence (1.6%) (Garbacz et al 2013).…”

Section: Staphylococcus Intermedius Groupmentioning

confidence: 99%

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

González-Martín

Corbera

Suárez‐Bonnet

et al. 2020

Veterinary Quarterly

View full text Add to dashboard Cite

Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors-molecules that allow bacteria to establish on or within a host and increase their ability to cause disease. These virulence factors have been widely described in S. aureus but information about other species of CoPS is scarce. The aim of this paper is to review the recent literature about the virulence factors of non-aureus CoPS of animal origin. Their possible effects on human health are also described. The role and prevalence of different virulence factors including leukocidins, hemolysins, adhesins, enterotoxins, exfoliative and toxic shock syndrome toxins as well as superantigen-like proteins are addressed. The effect of these virulence factors on human health is also described. The possibility of misdiagnosis of species of CoPS has been demonstrated in human clinical samples. Prevalence of zoonotic infections could be higher than thought and medical laboratories should be aware of these other staphylococcal species. In keeping with the 'One Health' approach to animal and human disease, medical professionals, veterinarians and health workers should be aware of the risks derived from exposure to these bacteria in people in close contact with animals, including pet owners, farmers and veterinarians themselves.

show abstract

“…Staphylococci have an array of potential virulence factors but despite detailed investigation significant associations between specific virulence genes and disease have not yet been identified, shifting attention again to host factors that may facilitate infection (Bannoehr et al, 2012;Tanabe et al, 2013;Couto et al, 2015). However, biofilm production, which can promote resistance to host defence mechanisms and greatly enhance antimicrobial resistance, has been confirmed in many isolates of S. pseudintermedius and other veterinary staphylococci (Götz, 2002;Hall-Stoodley et al, 2004).…”

Section: Pathogensmentioning

confidence: 99%

What has changed in canine pyoderma? A narrative review

Loeffler

Lloyd

2018

The Veterinary Journal

View full text Add to dashboard Cite

Prevalence of staphylococcal enterotoxins inStaphylococcus pseudintermediusisolates from dogs with pyoderma and healthy dogs

Cited by 18 publications

References 31 publications

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

What has changed in canine pyoderma? A narrative review

Contact Info

Product

Resources

About