Prevalence of Subclinical Mastitis in some Dairy Cattle Farms in Kuwait

Abdel-Khalek, A. E.; Alanzi, J

doi:10.21608/mvmj.2019.22.108

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Resistance to antimicrobials continues to compromise disease-prevention strategies and to increase the production and economic losses in animalbased food systems. Among the common conditions manifested at the farm level, mastitis is one of the frequent conditions of dairy animals [1]. This disease affects the udders of the animals, leading to a drop in milk quantity and quality, which significantly affects the farm's profitability [2].…”

Section: Introductionmentioning

confidence: 99%

Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica

Zou,

Hassan,

Akram

et al. 2023

Microorganisms

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This study focused on the assessment of the antimicrobial resistance of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) isolated from bovine mastitis milk samples and the revealing anti-mastitis potential of phytocompounds of Ziziphus jujube and Acacia nilotica through molecular docking analysis. The mastitis milk samples were collected from various dairy farms for the isolation of the bacteria (S. aureus and E. coli) and their response to antibiotics. Ethanolic extracts of both plants were prepared. Their antibacterial activity was evaluated, and they were processed for phytochemical analysis after which, molecular docking analysis with pathogenic proteins of the bacteria was carried out. Parametric and non-parametric statistical analyses were performed to reach the conclusions of this study. The findings of the study revealed a higher drug resistance (≥40%) of E. coli against ampicillin, amikacin, and vancomycin, while S. aureus exhibited the highest resistance to ampicillin, erythromycin, and ciprofloxacin. The ethanolic extracts of the Ziziphus jujube and Acacia nilotica plants produced a ZOI between 18 and 23 mm against multidrug-resistant S. aureus and E. coli. Gas chromatography–mass spectrophotometry (GC–MS) was used to explore 15 phytocompounds from Ziziphus jujube and 18 phytocompounds from Acacia nilotica. The molecular docking analysis of 2cyclopenten−1-one,3,4,4 trimethyl and Bis (2ethylhexyl) phthalate of Ziziphus jujube showed a binding affinity of −4.8 kcal/mol and −5.3 kcal/mol and −5.9 kcal/mol and −7.1 kcal/mol against the DNA Gyrase and toxic shock syndrome toxin-1 proteins of S. aureus and E. coli, respectively. The suberic acid monomethyl ester of Acacia nilotica showed a binding affinity of −5.9 kcal/mol and −5 kcal/mol against the outer membrane protein A and Topoisomerase IV protein of E. coli and −5.1 kcal/mol and −5.8 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus. Similarly, 2,2,4-trimethyl-1,3-pentanediol di-iso-butyrate showed a binding affinity of −6.5 kcal/mol and −5.3 kcal/mol against the outer membrane protein A and Topoisomerase IV of E. coli and −5.2 kcal/mol and −5.9 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus, respectively. The study concluded that there was an increasing trend for the antimicrobial resistance of S. aureus and E. coli, while the Ziziphus jujube and Acacia nilotica plant extracts expressed significant affinity to tackle this resistance; hence, this calls for the development of novel evidence-based therapeutics.

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Section: Introductionmentioning

confidence: 99%

Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica

Zou,

Hassan,

Akram

et al. 2023

Microorganisms

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Prevalence, Diagnosis and Improving the Effectiveness of Therapy of Mastitis in Cows of Dairy Farms in East Kazakhstan

Mukhamadieva

Julanov²,

Zainettinova³

et al. 2022

Veterinary Sciences

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In the present work, the prevalence, etiological factors and effective treatment scheme of mastitis in cows of dairy farms “Balke” and “Madi-R” in Eastern Kazakhstan were investigated. In total, 210 heads were investigated on two farms. The incidence of mastitis in cows on dairy farms is not the same in different years. Average clinical mastitis was detected in 35.4% of cows in 2016, 19.6% in 2017, 28.5% in 2018, and in 2019 in 16.4% of cows. The prevalence rates of subclinical mastitis by year had some differences. So, in 2016—36.5% of cows, then in 2017—21.5%, 2018—19.3% and in 2019—22.6%. In cows with udder inflammation, serum calcium 9.37 ± 0.15 mg/% with a range of 8.0 to 10.8 mg/%, phosphorus 3.58 ± 0.07 mg/% (3.0 to 4.3 mg/%), reserve alkalinity 363.46 ± 6.69 mg/% (320 to 440), carotene 0.49 ± 0.03 mg/% (0.220 to 0.988 mg/%), which are in the lower limit of physiological parameters. The drug “Dorob” was tested during the study of comparative effectiveness of treatment methods. The results of the study showed that this drug has anti-inflammatory, antimicrobial and stimulating healing actions. The treatment of the sick cows with catarrhal mastitis has shown that a total of 8 cows have recovered in the control group and 10 cows in the experimental group with the preparation “Dorob”. The period of recovery in the control group was 8.8 ± 0.39, and in the experimental group—6.2 ± 0.28 (p < 0.05). The drug does not contain antibiotics and hormonal preparations. The inclusion of the drug in the scheme of treatment allows for effectively treating inflammatory processes in the udder of cows and restoring their productivity with minimal cost of time and money.

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Prevalence of Subclinical Mastitis in some Dairy Cattle Farms in Kuwait

Cited by 2 publications

References 13 publications

Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica

Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica

Prevalence, Diagnosis and Improving the Effectiveness of Therapy of Mastitis in Cows of Dairy Farms in East Kazakhstan

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