Prevalence of ten putative virulence genes in the emerging foodborne pathogen Arcobacter isolated from food products

Girbau, Cecilia; Guerra, Cristian; Martínez-Malaxetxebarria, Irati; Alonso, Rodrigo; Fernández‐Astorga, Aurora

doi:10.1016/j.fm.2015.07.015

Cited by 47 publications

(62 citation statements)

References 30 publications

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“…) but very similar to that reported in the only study performed on dairy isolates (Girbau et al . ). The present study detected the iroE gene in 30% of isolates, in line with only three reports investigating the same gene that showed prevalence rates of 17% (Girbau et al .…”

Section: Discussionmentioning

confidence: 97%

“…(2011) with the exception of the IroE gene that was detected using the protocol described by Girbau et al . (). Arcobacter butzleri DSM 8739 T was used as a positive control and sterile water as a negative control.…”

Section: Methodsmentioning

confidence: 97%

“…Some studies showed a different prevalence of putative virulence genes in relation to the source of the strain (Girbau et al . ; Lehmann et al . ; Douidah et al .…”

Section: Introductionmentioning

confidence: 99%

“…However, only one study is available on the prevalence of putative virulence genes in Arcobacter isolated from dairy samples (Girbau et al . ), while none to our knowledge have investigated the presence of Arcobacter putative virulence genes in different environmental sites. The present study aimed to investigate and compare virulence genotypes of A. butzleri isolates derived from three different environmental sites linked to the dairy production chain: the farm, the primary production site and industrial and artisanal dairy plants representing two different dairy processing environments well represented in Italy.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

et al. 2017

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

“…Some studies showed a different prevalence of putative virulence genes in relation to the source of the strain (Girbau et al . ; Lehmann et al . ; Douidah et al .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

et al. 2017

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“…having the potential to adhere to and invade several cell lines including human intestinal epithelial cells causing cytotoxicity Levican et al, 2013c;Ferreira et al, 2015;Karadas et al, 2016). Putative virulence genes have been identified in various Arcobacter species/strains in numerous studies (Douidah et al, 2012;Levican et al, 2013c;Ferreira et al, 2015;Girbau et al, 2015;Piva et al, 2017). Further work is required to better understand the relationship between these putative virulence markers and human clinical outcomes, though some tantalizing evidence suggests that Arcobacter has the potential to induce tight junction dysfunction (Bücker et al, 2009;Karadas et al, 2016), which may lead to diarrhea .…”

Section: Routes Of Transmissionmentioning

confidence: 99%

Arcobacter

Banting¹,

Salvat²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Lameei

Rahimi

Shakerian

et al. 2022

Veterinary Medicine & Sci

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Background Arcobacter spp. has been considered an emerging foodborne pathogen and a hazard to human health. The dairy chain has been isolated from different sources; nevertheless, data on Arcobacter occurrence in raw milk and dairy products in Iran are still scant. Objective The present study investigates the prevalence, antimicrobial susceptibility and the presence of virulence genes of Arcobacters species isolated from milk and dairy products. Methods Then, a total of 350 raw milk samples and 400 dairy product samples were collected from dairy supply centers in Isfahan, Iran. Presumptive Arcobacter strains were obtained by enriching samples in Oxoid Arcobacter enrichment broth (AEB) followed by the filtration of enrichment product through 0.45‐μm pore size membrane filters laid onto non‐selective blood at 30°C under microaerophilic conditions. Molecular identification of Arcobacter cryaerophilus and A. butzleri was performed by Polymerase chain reaction (PCR) amplification of the 16S rRNA gene, followed by sequencing. The disc diffusion method was used to determine the antimicrobial susceptibility of isolates. Targeted resistance and virulence genes were detected using multiplex PCR. Results The results show a low recovery rate of Arcobacter spp. in milk. Arcobacters were found in all types of milk, except raw camel milk, but were absent from all dairy products. Arcobacter butzleri was the predominant species in raw milk. Detection of virulence genes shows that all virulence genes targeted were found among A. butzleri, and six (cadF, cj1349, irgA, mviN, pldA, tlyA) were found among A. cryaerophilus. All A. butzleri strains and some A. cryaerophilus strains isolated from milk were resistant to amoxicillin‐clavulanic acid and tetracycline. All A. cryaerophilus isolates from milk were susceptible to gentamycin, streptomycin, erythromycin and ciprofloxacin. The distribution of resistance genes in Arcobacter strains in milk shows that all isolates carried tet(O) and blaOXA‐61 genes. Conclusions In conclusion, the results indicate a low recovery rate of Arcobacter spp. in milk and milk products. However, a significant number of Arcobacter strains with putative virulence genes may be potential pathogens for humans and an overall increase in Arcobacter resistance to first‐line antibiotics. These results highlight the need for regular surveillance of Arcobacter strains in milk and milk products in Iran.

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Prevalence of ten putative virulence genes in the emerging foodborne pathogen Arcobacter isolated from food products

Cited by 47 publications

References 30 publications

Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

Arcobacter

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

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