Prevalence of the Fimbrial Antigens F18 and K88 and of Enterotoxins and Verotoxins among Escherichia coli Isolated from Weaned Pigs

Wittig, W; Klie, H.; Gallein, Peter; Lehmann, Simone; Timm, M.; Tschäpe, Helmut

doi:10.1016/s0934-8840(11)80895-9

Cited by 47 publications

(44 citation statements)

References 10 publications

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“…The fimbrial antigen F18 is one of the most important fimbriae associated with PWD (9,16,25). In this study we found the gene for F18 in 38% of E. coli strains examined, whereas 27.5% and 19% of F18-positive E. coli strains were found in the Santa Catarina and Minas Gerais States, respectively (6,14).…”

Section: Discussionmentioning

confidence: 54%

“…In ETEC strains, the fimbrial types F4 (K88) and F18 (F18ab, F18ac) are commonly found in pathogenic E. coli isolated from weaned pigs (4,18,25). There are also other fimbrial structures, such as F5 (K99), F6 (987P), F41, which are associated with porcine ETEC strains, but are more frequently associated with E. coli causing neonatal diarrhea (18,24).…”

Section: Introductionmentioning

confidence: 99%

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Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Vidotto¹,

Lima²,

Fritzen³

et al. 2009

Braz. J. Microbiol.

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Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Vidotto¹,

Lima²,

Fritzen³

et al. 2009

Braz. J. Microbiol.

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“…Enterotoxigenic Escherichia coli serotypes with adhesin F4 (or K88) are frequently found to be causative agents of post-weaning diarrhoea (PWD) in piglets [1][2][3]. PWD causes diminished animal health and also causes economic losses for the farmer, due to increased mortality and growth retardation.…”

Section: Introductionmentioning

confidence: 99%

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Geenen

Meulen²,

Bouma

et al. 2004

Epidemiol. Infect.

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SUMMARY F4+Escherichia coli is an important agent of post-weaning diarrhoea in piglets. Piglets that express an adhesion site for F4+ E. coli in their small intestine (F4R+) shed higher numbers of F4+ E. coli than piglets lacking this site (F4Rx). We hypothesized that F4R+ piglets are more infectious and more susceptible for F4+ E. coli. This implies that in populations with F4R+ and F4Rx piglets, the transmission would be dependent on the frequency of both types of animals. To quantify the difference in infectiousness and susceptibility, a one-to-one transmission experiment was performed with 20 pairs consisting of one inoculated and one contact piglet. Based on the contact infections observed, transmission parameters were estimated with generalized linear models. F4R+ piglets were infectious for other piglets and the reproduction ratio (R 0 ) for homogeneous F4R+ populations, that is the average number of secondary infections that one F4R+ pig will cause during its entire infectious period in a population of susceptible F4R+ individuals only, was estimated as 7 . 1. F4R+ piglets were more susceptible than F4Rx piglets and reducing the fraction of F4R+ piglets of a population will reduce transmission. It was calculated that in order to prevent major outbreaks of F4+ E. coli (R 0 <1), the fraction of F4R+ piglets must be lower than 0 . 14.

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“…Several adherence factors have been described and enterotoxigenic E. coli strains with adhesin F4 (or K88) have been reported as the main agents of PWD in several countries [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Transmission of F4+ E. coli in groups of early weaned piglets

Geenen

Döpfer²,

Meulen³

et al. 2004

Epidemiol. Infect.

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SUMMARYThe aim of this study was to estimate transmission parameters of enterotoxigenic F4+ Escherichia coli F4 (F4+ E. coli) in groups of early weaned piglets with F4-receptor-positive (F4R+) and F4-receptor-negative piglets (F4Rx). Transmission of F4+ E. coli was quantified in four heterogeneous groups of F4R+ and F4Rx piglets. Infectiousness was determined by the number of F4+ E. coli/g faeces shed during 8 days. Transmission parameters were estimated using generalized linear models assuming a stochastic SIR model. F4R+ piglets were found to be more susceptible than F4Rx piglets, but F4R+ and F4Rx piglets were not different in infectiousness. The reproduction ratios for homogeneous F4R+ and F4Rx populations were estimated as 6 . 37 (95 % CI 1 . 89-21 . 48) and 0 . 02 (95 % CI 0 . 00-1 . 13) respectively. The implication of these results is that in order to prevent major outbreaks, the fraction of F4R+ piglets should be small (approximately 10 % or less). Therefore, selective breeding programmes could contribute to reducing F4+ E. coli-related diarrhoea and transmission.

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Prevalence of the Fimbrial Antigens F18 and K88 and of Enterotoxins and Verotoxins among Escherichia coli Isolated from Weaned Pigs

Cited by 47 publications

References 10 publications

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Estimating transmission parameters of F4+ E. coli for F4-receptor-positive and -negative piglets: one-to-one transmission experiment

Transmission of F4+ E. coli in groups of early weaned piglets

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