Prevalence of Toxoplasma gondii in cats from Colombia, South America and genetic characterization of T. gondii isolates

Dubey, J. P.; Su, Chunlei; Cortés, Jorge Alberto; Natarajan, Sundar; Gómez-Marín, Jorge Enrique; Polo, Luis; Zambrano, L.; Mora, Luciano; Lora, Fabiana; Jiménez, Juan E.; Kwok, O. C. H.; Shen, S. K.; Zhang, X.; Nieto, Alberto; Thulliez, P.

doi:10.1016/j.vetpar.2006.04.037

Cited by 96 publications

(49 citation statements)

References 10 publications

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“…In Europe, a majority of isolates are from genotype II [13,17], whereas in Brazil and Colombia, isolates from chickens genotyped at SAG2 locus were reported to be from genotypes I and III but not from genotype II [18,19]. Of 15 isolates from Colombian cats genotyped at five different loci, only one was found to be genotype II and 11 displayed a combined type I and III genotype [20]. The rarity of genotype II isolates in both Colombia and Brazil favors a different Toxoplasma population structure in South America than in Europe, as reported by Lehmann et al [15].…”

Section: Discussionmentioning

confidence: 99%

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Morisset

Peyron

Lobry

et al. 2008

Microbes and Infection

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Section: Discussionmentioning

confidence: 99%

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Morisset

Peyron

Lobry

et al. 2008

Microbes and Infection

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“…In the present study, we adopt a cut-off value equal to 1:20 for canine and feline sera both by IFAT and MAT (Claus et al 1977;Lindsay et al 1997;Mineo et al 2001;Silva et al 2002;Dubey et al 2004Dubey et al , 2006Dubey et al , 2007aPiergili Fioretti 2004;Afonso et al 2006). …”

Section: Cut-offmentioning

confidence: 99%

Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat

et al. 2009

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The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.

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“…are protozoan parasites that cause zoonosis, are distributed worldwide, and affect domestic and wild animals and humans. Cats are important in the epidemiology of toxoplasmosis because they are the only hosts that can excrete the environmentally resistant stage: the oocysts 1 .…”

mentioning

confidence: 99%

Coinfection by Toxoplasma gondii and Leishmania spp. in domestic cats (Felis catus) in State of Mato Grosso do Sul

Braga¹,

Corrêa

Camossi

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Toxoplasma gondii and Leishmania spp. are protozoan parasites that cause zoonosis, are distributed worldwide, and affect domestic and wild animals and humans. Cats are important in the epidemiology of toxoplasmosis because they are the only hosts that can excrete the environmentally resistant stage: the oocysts 1 .In 1999, visceral leishmaniasis in cats was fi rst recorded by Hervas et al. 2 in Spain. Since then, current cases represent a widespread distribution, and include reports from Italy 3 , Portugal 4 , the United States 5 . In Brazil, the fi rst occurrence of Leishmania infantum in a cat was recorded in 2004 by Savani et al 5 . Some authors consider this animal an accidental host, while others suggest that felids can act as potential peridomestic reservoirs 6 . However, the role of cats in the epidemiology of leishmaniasis remains to be clarifi ed.The present study was approved by the Animal Ethics Committee (protocol 65/2007) of the School of Veterinary Medicine and Animal Husbandry, São Paulo State University, following the Ethical Principles in Animal Experimentation. A total of 50 cats (Felis catus) that were domiciliated in the City of Campo Grande (20°26'34''S, 54°38'47''W) in the State of Mato Grosso do Sul, Brazil were selected randomly without predilection for sex breed, or age. Blood samples were obtained to detect antibodies for T. gondii and Leishmania spp.The immunofl uorescence antibody test (IFAT) was performed, according to the method described by Camargo 7 , which used whole Leishmania major-like promastigote and the RH strain of T. gondii as the antigen for the diagnosis of leishmaniasis and toxoplasmosis, respectively. Modifi ed agglutination test (MAT) using the RH strain was performed, as described by Desmonts and Remington 8 . The cutoff T. gondii antibody titer for both tests was 16, and the corresponding value in IFAT for Leishmania spp. was 40.Two (4%) of the 50 animals presented titers of antibodies for T. gondii and Leishmania spp., which suggested coinfection by these two protozoa in the same animal. Cat #1 was a female of undefi ned breed, 2 years of age, and presented T. gondii antibody titers of 64 (based on IFAT) and 1,024 (based on MAT), and an antibody titer of 320 for Leishmania spp. Cat #2 was also a female of undefi ned breed, 3 years of age, and presented T. gondii titers of 16 (based on IFAT) and 1,024 (based on MAT), and a Leishmania spp. titer of 320 (Table 1). Neither cat presented clinical signs. Similar results were obtained by Cardia et al. 9 with specifi c anti-Toxoplasma immunoglobulin G (IgG) in 63 (16.3%) of 386 cats and IgG against Leishmania spp. in two serum samples with titers of 1:160 and 1:320, respectively. In a study conducted by Sobrinho et al. 10 in another Brazilian endemic area, 25.7% cats were coinfected with both agents. However, the authors did not observe a signifi cant association between these infections.In this study, the MAT and IFAT were used, based on the results for cats obtained by Macrì et al. 11 , with a concordance of 0.98 (i.e...

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Prevalence of Toxoplasma gondii in cats from Colombia, South America and genetic characterization of T. gondii isolates

Cited by 96 publications

References 10 publications

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat

Coinfection by Toxoplasma gondii and Leishmania spp. in domestic cats (Felis catus) in State of Mato Grosso do Sul

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