Prevalence of Tuberculosis in Cattle and Buffalo at Various Livestock Farms in Punjab

Ghumman, Manzoor Ahmed; Manzoor, Abdul Whab; Naz, Sarwat; Ahmad, Rais; Buddle, Bryce M.

doi:10.5171/2013.145084

Cited by 7 publications

(9 citation statements)

References 5 publications

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“…(Ghumman et al 2013)in their study reported the prevalence of tuberculosis in cattle and buffaloes were reported (11.71%) and (11.96%) respectively. Results of the present study showed similarity with the results of (Ghumman et al 2013), showed the prevalence of TB was insignificantly different in both cattle and buffaloes. (Hena et al 2012) in their study reported the prevalence of tuberculosis in sheep and goats were (0.99%) and (1.34%) respectively.…”

Section: Sensitivity and Specificity Of Different Techniquesmentioning

confidence: 99%

“…Tuberculosis (TB) is a chronic, contagious and infectious disease of domestic animals as well as humans, and wild animal species, caused by a group of interrelated acid fast bacteria, forming the Mycobacterium tuberculosis complex (Ghumman et al, 2013 Thoen et al, 2009). M. tuberculosisis the principal microorganism related with human tuberculosis whereas M. bovis is most important in causing bovine tuberculosis (BTB) (Tenguria et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Basit

Hussain

Ayaz

et al. 2015

IJAR

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Tuberculosis is a worldwide contagious and chronic disease of human as well as domestic animals with zoonotic potential. The Mycobacterium bovis and Mycobacterium tuberculosis are the main cause of tuberculosis. It has worldwide distribution with significant effect on animals and has public health importance. Therefore the present study was conducted to determine the prevalence of Mycobacterium bovis and Mycobacterium tuberculosis among the ruminant of district Kohat, Khyber PakhtunKhwa and also to evaluate the sensitivity and specificity of Microscopy and PCR. A total 200 tissue samples of lungs, lymph nodes and liver from cattle, buffaloes, sheep and goats were collected from Abattoir Kohat. All the tissue were first examined by direct smear microscopy by Ziehl Neelsen staining and then subjected to the PCR for the detection of M. bovis and M. tuberculosis. The overall prevalence of tuberculosis was 6.5% by PCR.Prevalence of tuberculosis was recorded in7.87% of lungs samples followed by 5.26% lymph node. Moreover the prevalence was found 5.2%, 4%, 10.6% and 6.5% in cattle, buffalos, Goats and sheep respectively. Furthermorethe sensitivity and specificity of PCR and microscopy in term of detection of TB was determined that PCR was found less sensitive then microscopy because of other species which was not amplified due to non availability of specific primer and were found positive in microscopy.In conclusion PCR is more reliable diagnostic tool for diagnosis of bovine tuberculosis. It is recommended that PCR based diagnostic reference laboratory may be established at district level and Tuberculosis awareness campaign must be arranged.

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Section: Sensitivity and Specificity Of Different Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Basit

Hussain

Ayaz

et al. 2015

IJAR

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“…In another study by Javed et al (2008), incidence of bTB was recorded as 8.48% in buffaloes at animal experimental station, Pakistan, which is also in-line to our findings. Ghumman et al (2013) has stated higher prevalence of bTB as 11.71% in cattle population at several dairy farms, Punjab Pakistan. On the other hand, many scientists have documented slightly lower incidence of bTB in buffaloes such as 3% by Javed et al, 2010, 2.2% by Javed et al, 2009 and 1.7% by Ifrahim, 2001 while several researchers have reported a little greater prevalence in buffaloes such as 9.6% by Mumtaz et al, 2008, 10.6% by Khan et al, 2008, 11.3% by Javed et al, 2012 and12.72% by Khan and Khan, 2007.…”

Section: Resultsmentioning

confidence: 99%

Bovine Tuberculosis (bTB)-Isolation and Species-Specific Identification of Mycobacterium bovis from Bovine Raw Milk in Pakistan

Ullah¹,

Sadique²,

Ayaz³

et al. 2020

SJA

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T uberculosis (TB) is a chronic infectious disease of both human and animals. Mycobacterium tuberculosis (M. tuberculosis) is the etiological agent of TB in human (Olea-Popelka et al., 2017) while Mycobacterium bovis (M. bovis) causing TB in animals (Grange, 1998) commonly called bovine tuberculosis (bTB). Bovine tuberculosis is one of the multifaceted, stubborn and debatable problems in front of world Abstract | A research study was carried out to investigate the prevalence of bovine tuberculosis (bTB); detection of Mycobacterium bovis (M. bovis) infection in lactating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalence of bovine tuberculosis. A total of 1608 milk samples (793 from cattle and 815 from buffaloes) were collected from already PPD (purified protein derivatives) tested lactating animals aseptically. The data obtained were finally analyzed statistically using chi squared test. The Mycobacterium was identified through ZN staining, culture and PCR. Out of 1608 milk samples, 60 (3.73%) were found positive for acid fast bacteria through ZN staining whereas the prevalence of Mycobacterium bovis was confirmed in 65 (4.04%) and 85 (5.29%) isolates through culture and PCR respectively. The zoonotic spread of M. bovis infection was established in the study area. The data determines that animal food products in Pakistan are common reservoirs of mycobacteria and may pose great risk to the public. Bovine tuberculosis (bTB) is found one of the neglected zoonotic disease (NZD) caused by Mycobacterium bovis with public health significance and appear as a life threatening infection at the human-animal-ecosystem interface in low-resource settings in the central zone of KP, Pakistan. The surveillance, prevention and control programs of this NZD in the KP province of Pakistan is necessary because of the continual unrestricted movement of infected animals and the use of unpasteurized milk which might result in an increased spread of bTB to human population.

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“…Another critical and contributing factor for the spread of the disease to humans is especially through farmers, dairy workers associated with poorly ventilated houses. Furthermore, the individuals have close contact with the animals during feeding/milking, also lived near the carrier animals are more susceptible to the disease (Khan and Khan 2007;Ghumman et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Tuberculosis (TB) caused by Mycobacterium tuberculosis is an infectious and chronic debilitating disease of humans, domestic animals as well as wild animals, which significantly prevails throughout the world (Ghumman et al, 2013). M. tuberculosis is the principal microorganism related with human tuberculosis, whereas M. bovis is the most important pathogen causing bovine tuberculosis (BTB) and has great potential to infect human and other animals due to their broad range of host (Khan and Khan, 2007).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Risk Factors Associated with Mycobacterium tuberculosis and Mycobacterium bovis in Milk Samples from North East of Pakistan

Basit¹

2018

PVJ

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Tuberculosis is a chronic, debilitating and highly contagious zoonotic disease threatening animals and public health. This study was designed to investigate the occurrence of Mycobacterium tuberculosis and Mycobacterium bovis and to identify the risk factors associated with the disease in milk samples of dairy animals. A total of 200 milk samples were collected from lactating animals and milk shops of five randomly selected union councils of district Kohat, Pakistan. The epidemiological and herd management data was collected on a pre-designed questionnaire. All milk samples were first screened by direct microscopy, followed by molecular detection of M. tuberculosis and M. bovis using PCR assay. Overall, PCR based prevalence of tuberculosis causing bacteria was 7.5%. The high prevalence rate of M. bovis was found in cattle (6.4%), followed by buffaloes (6.2%), while the prevalence of M. tuberculosis was higher in goats (6.3%). A higher prevalence of M. bovis (15%) was found in the Khushal Ghar area. Moreover, the prevalence was also high in milk samples collected from milk shops (7.4%). Additionally, animal herd size and hygienic measures significantly (P<0.05) contributed to the prevalence rate. The prevalence rate in the age group of >8 years was found higher compared to other age groups. Furthermore, the high prevalence rate was found in animals having close contact with other animals in communal grazing and watering. This study concluded that some factors influence significantly in the prevalence of tuberculosis. Thus, actual managemental practices might be helpful in control and prevention of these pathogenic bacteria.

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Prevalence of Tuberculosis in Cattle and Buffalo at Various Livestock Farms in Punjab

Cited by 7 publications

References 5 publications

Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Bovine Tuberculosis (bTB)-Isolation and Species-Specific Identification of Mycobacterium bovis from Bovine Raw Milk in Pakistan

Occurrence and Risk Factors Associated with Mycobacterium tuberculosis and Mycobacterium bovis in Milk Samples from North East of Pakistan

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