Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Basit, Abdul; Hussain, Mubbashir; Ayaz, Sultan; Shahid, Muhammad; Rahim, Kashif; Ahmad, I.; Ullah, Riaz; Hashem, Abeer; Abd-Allah, E M; Alqarawi, Abdulaziz A.; Gul, Naila

doi:10.18805/ijar.5583

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…The same results as of present study was reported by Basit et al (2015) [22] with 10.6% in goat from the clinical sample and on the lower side with Ashenafi et al (2013) [23] with 25% of prevalence in clinical samples from goats. The Mycobacteria have been more frequently identified in blood than in milk samples (Carvalho et al, 2014, Romero et al, 1999, Srivastava et al, 2008) [24,25,26] .…”

Section: Discussionsupporting

confidence: 92%

Molecular detection of Mycobacterium bovis in goats from Nagpur region of Maharashtra

Sonekar¹,

Patil²,

Fusey³

et al. 2021

Pharma Innovation

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Tuberculosis is a zoonotic disease caused by bacteria; Mycobacterium species. It is distributed worldwide and have the economic and public health significance. The present study was carried out to detect and identify the presence of Mycobacterium pathogens using polymerase chain reaction in blood samples from goats. Blood samples from the total of 236 goats suspected of tuberculosis as well as apparently healthy were collected from in and around Nagpur region. The DNA in blood sample was assessed by PCR amplification of IS6110 and RD1, RD4 gene to detect Mycobacteria at genus and species level respectively. The overall positivity of M. bovis among goats was recorded as 10.59% (25/236). The study demands thorough testing of the flocks for continuous monitoring for tuberculosis for preventing its further spread.

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Section: Discussionsupporting

confidence: 92%

Molecular detection of Mycobacterium bovis in goats from Nagpur region of Maharashtra

Sonekar¹,

Patil²,

Fusey³

et al. 2021

Pharma Innovation

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“…Nevertheless, when the isolate is from clinical samples such as tubercle nodule, it is strongly recommended to follow the heat kill step. DNA isolation is always a limiting factor for mycobacterial research (Basit et al, 2015), even most of the commercial kits need to be modified for mycobacerial DNA isolation atleast for the initial lysis step (Fell et al 2016;Radomski et al 2013). The CTAB method practiced for Mycobacterial DNA isolation still stood good and in our case also, it fulfilled the requirement for WGS.…”

Section: Resultsmentioning

confidence: 99%

“…Nowadays with increase reports of its isolation from various human infections it is being suspected as a potential zoonotic pathogen also (Falkinham, 2011). Identification of M. chelonae is done conventionally by culture and biochemical methods which are laborious and restricted to reference laboratories only as that of other Mycobacterial organism like Mycobacterium tuberculosis, M.bovis etc (Basit et al, 2015). The popularity of molecular and proteomics techniques like RFLP-hsp65, sequencing of hsp65 gene (Telenti et al 1993), rpoB gene (Ade´kambi et al 2003), 16sRNA (Pascual et al 1995), MALDI-TOF (Genc et al 2018) had given additional advantage to many laboratories other than reference also to confirm these isolates.…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Sequencing and Analysis of Mycobacteroides chelonae M77 Isolated from Cow Milk from the Hill State of Meghalaya, India

Vise

Ghatak

Garg

et al. 2021

IJAR

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Background: Mycobacteroides chelonae M77 isolated in a targeted Mycobacterial isolation study from bovine raw milk was whole genome sequenced (CP041150.1), followed by comparative genomics study for better understanding of the organism with special focus on its virulence and antibiotic resistance pattern. This is probably the first whole genome sequence (WGS) of M.cheloane submitted in NCBI database from India. Methods: The WGS has been carried out in Illumina Next Seq 500 platform and then assembled resulting in a genome of 5.12 MB. The assembled genome was annotated in RASTtk and viewed by SEED for its detailed analysis. Pathogen Finder 1.1 and Res Finder 3.1 soft wares were applied for knowing the isolate pathogenecity and antibiotic profiling respectively. Mauve analysis was carried out to know the genomic re-arrangements.Result: Pathogen Finder 1.1 showed that M77 was non-pathogenic and Res Finder 3.1 server reveals it is susceptible to most of the antibiotics. Mauve analysis for alignment of isolate revealed no significant re-arrangement of genomic segment. The whole analysis showed that the genome of M77 is tight genome with not much variation in relation to previous complete genomes reported and is non-pathogenic and sensitive to most of the antibiotics.

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“…It has been reported that 3% of all human tuberculosis cases are caused by M. bovis, through close contacts with livestock or consumption of raw milk and its products (Belchior et al, 2016). Recently, we reported the prevalence of TB from tissue samples of ruminants collected from abattoir of the district, Kohat (Basit et al, 2015).…”

Section: Research Articlementioning

confidence: 99%

Occurrence and Risk Factors Associated with Mycobacterium tuberculosis and Mycobacterium bovis in Milk Samples from North East of Pakistan

Basit¹

2018

PVJ

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Tuberculosis is a chronic, debilitating and highly contagious zoonotic disease threatening animals and public health. This study was designed to investigate the occurrence of Mycobacterium tuberculosis and Mycobacterium bovis and to identify the risk factors associated with the disease in milk samples of dairy animals. A total of 200 milk samples were collected from lactating animals and milk shops of five randomly selected union councils of district Kohat, Pakistan. The epidemiological and herd management data was collected on a pre-designed questionnaire. All milk samples were first screened by direct microscopy, followed by molecular detection of M. tuberculosis and M. bovis using PCR assay. Overall, PCR based prevalence of tuberculosis causing bacteria was 7.5%. The high prevalence rate of M. bovis was found in cattle (6.4%), followed by buffaloes (6.2%), while the prevalence of M. tuberculosis was higher in goats (6.3%). A higher prevalence of M. bovis (15%) was found in the Khushal Ghar area. Moreover, the prevalence was also high in milk samples collected from milk shops (7.4%). Additionally, animal herd size and hygienic measures significantly (P<0.05) contributed to the prevalence rate. The prevalence rate in the age group of >8 years was found higher compared to other age groups. Furthermore, the high prevalence rate was found in animals having close contact with other animals in communal grazing and watering. This study concluded that some factors influence significantly in the prevalence of tuberculosis. Thus, actual managemental practices might be helpful in control and prevention of these pathogenic bacteria.

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Isolation and identification of Mycobacterium bovis and Mycobacterium tuberculosis from animal tissues by conventional and molecular method

Cited by 6 publications

References 13 publications

Molecular detection of Mycobacterium bovis in goats from Nagpur region of Maharashtra

Molecular detection of Mycobacterium bovis in goats from Nagpur region of Maharashtra

Whole Genome Sequencing and Analysis of Mycobacteroides chelonae M77 Isolated from Cow Milk from the Hill State of Meghalaya, India

Occurrence and Risk Factors Associated with Mycobacterium tuberculosis and Mycobacterium bovis in Milk Samples from North East of Pakistan

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