Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss.

Tuberculosis is a zoonotic disease caused by bacteria; Mycobacterium species. It is distributed worldwide and have the economic and public health significance. The present study was carried out to detect and identify the presence of Mycobacterium pathogens using polymerase chain reaction in blood samples from goats. Blood samples from the total of 236 goats suspected of tuberculosis as well as apparently healthy were collected from in and around Nagpur region. The DNA in blood sample was assessed by PCR amplification of IS6110 and RD1, RD4 gene to detect Mycobacteria at genus and species level respectively. The overall positivity of M. bovis among goats was recorded as 10.59% (25/236). The study demands thorough testing of the flocks for continuous monitoring for tuberculosis for preventing its further spread.

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