Prevalence of vero toxic Escherichia coli in fecal samples of domestic as well as wild ruminants in Mathura districts and Kanpur zoo

Mishra, Raghavendra Prasad; Jain, Udit; Bist, Basanti; Verma, Anju; Kumar, Ashok

doi:10.14202/vetworld.2016.71-74

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Almost similar prevalence of VTEC has been reported in another study from India by Mishra et al . 13.4% [ 24 ] while higher prevalence values have been reported from other places such as Egypt (26.7%) [ 25 ], California (37.9%) [ 26 ], Ireland (40%) [ 27 ], and Florida (58.1%) [ 6 ]. The wide variations in prevalence values of E. coli and VTEC in calves feces can be attributed to epidemiological determinants such as stocking density, age, sex, and season, whereas sampling strategy, sample handling, and laboratory methods might also have profound effect on prevalence values reported in different countries [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

A study on association of virulence determinants of verotoxic Escherichia coli isolated from cattle calves

Parul¹,

Bist

Sharma

et al. 2016

Vet World

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Aim:The present study was conducted to find the association among virulence determinants of verotoxic Escherichia coli (VTEC) isolated from cattle calf feces.Materials and Methods:A total of 216 cattle calf fecal samples were collected aseptically and processed under required conditions for the isolation of E. coli. The isolates were further subjected to multiplex polymerase chain reaction (mPCR) for the detection of virulent genes. All the VTEC isolates were serotyped at the Central Research Institute, Kasauli, Himachal Pradesh. The VTEC isolates were observed for the enterohemolysin production on washed sheep blood agar (wSBA).Results:A total of 177 presumptive E. coli were isolated from 216 calf fecal samples revealing an overall prevalence of E. coli to be 81.94%. A total of 32 (14.81%) isolates were detected as VTEC through mPCR. The prevalence of verotoxin genes vt1, vt2, and combination of vt1+vt2 in the VTEC isolates was found to be 12 (37.5%), 14 (43.75%), and 6 (18.75%), respectively. Other virulent genes eaeA and hlyA were found in 6 and 11 VTEC strains with prevalence values of 18.75% and 34.37%, respectively. A total of 13 different O serogroups were revealed in serotyping of 32 VTEC isolates. Out of 32 VTEC strains, only 26 (81.25%) were enterohemolytic on wSBA as they produced the characteristic small, turbid zone of hemolysis around the streaking line. Although enterohemolysin production has been attributed to the presence of hlyA gene, only 11 of 26 enterohemolysin producing VTEC were found to be harboring the hlyA gene (11/26) 42.03%.Conclusion:The present study concludes that there might be an association between the presence of verotoxin genes and enterohemolysin production in VTEC group of E. coli.

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Section: Discussionmentioning

confidence: 99%

A study on association of virulence determinants of verotoxic Escherichia coli isolated from cattle calves

Parul¹,

Bist

Sharma

et al. 2016

Vet World

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“…[17] ishalli manda yavrularına ait 32 dışkı örneğinin 19'undan (%59.4) VTEC izolasyonu yapmışlardır. Hindistan'da gerçekleştirilen bir çalışmada [16] Hindistan'da mandalarda enteropatojenik E. coli (EPEC) epidemiyolojisi üzerine gerçekleştirilen bir çalışmada [23], 43 manda dışkısının 35'inden (%81.4) O157 olmayan E. coli serotipleri izole edilmiş, bunların sadece 1'i (%2.3) EPEC olarak tanımlanmıştır. İzolatların tamamı ampisilin, amoksisilin, tetrasiklin ve enrofloksasine duyarlı bulunmuştur.…”

Section: Tartışma Ve Sonuçunclassified

Samsun ili ve ilçelerinde yetiştirilen Anadolu mandalarının dışkı örneklerinde Escherichia coli O157:H7’nin tespiti

Nuhay

Gülhan

2017

Etlik Veteriner Mikrobiyoloji Dergisi

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Mandalar, pek çok hayvan türü gibi bazı hastalıkların duyarlı hayvan popülasyonlarına ve insanlara bulaştırılmasında rol oynamaktadır. Enterohemorajik Escherichia coli (EHEC) suşları, insanlarda hemorajik kolitis (HC) ve hemorajik üremik sendrom (HUS) başta olmak üzere ölümcül enfeksiyonlara neden olmaktadır. Bu enfeksiyonlardan sorumlu başlıca EHEC serotipinin E. coli O157:H7 olduğu ortaya konulmuştur. Bu araştırmada, Samsun ili ve ilçelerindeki Anadolu Mandalarına ait 1000 dışkı örneği E. coli O157:H7 açısından standart kültürel metot ile incelendi. İncelenen 1000 dışkı örneğinden 38 (%3.8) E. coli O157:H7 serotipi, 400 (%40) E. coli O157:H7 serotipi yönünden negatif E. coli olmak üzere toplam 438 (%43.8) E. coli izole ve identifiye edildi. İzolatların tamamı penisilin G ve eritromisine dirençli, danofloksasin, amokssisilin+klavulanik asit, sefaperazon, ampisilin+sulbaktam, oksitetrasiklin ve ampisiline duyarlı olarak bulundu. Bu araştırma ile bölgemizde ilk kez Anadolu Mandalarına ait dışkı örnekleri E. coli O157:H7 serotipi yönünden incelendi. Bu araştırmadan elde edilen verilerin, yöremizde yapılacak benzer çalışmalara kaynak teşkil edebileceği kanısına varıldı.

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“…Cattle are considered as the principal reservoir of VTEC O157:H7; therefore, it functions as a potential source for transmission of VTEC O157:H7 to humans by direct or indirect route [8]. VTEC has been isolated from healthy intestines of sheep, deer, and buffalo; the distribution is not restricted to a particular geographical area, and among the isolates, serotype O157:H7 is mostly associated with severe human infections [9]. The virulence attributed to O157:H7 is due to the secretion of Shiga toxins (Stx), which inhibits protein synthesis of the host cells, ultimately leading to cell death.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Escherichia coli serotype O157:H7 and other verotoxin-producing E. coli in healthy Indian cattle

et al. 2020

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Background and Aim: Cattle are the main reservoir of Escherichia coli O157:H7 and other verotoxigenic E. coli (VTEC); therefore, there is an increased risk of infection to humans by either direct or indirect mode of transmissions. However, the prevalence of E. coli O157:H7 in the healthy cattle population of India is yet to be ascertained. This study aimed to screen the dairy cattle in and around Pune, Maharashtra, India, for verotoxin-producing E. coli O157:H7. Materials and Methods: A total of 257 rectal swabs were collected from 15 different organized and unorganized dairy farms of Pune during the period, January-March 2015. The screening involved enrichment in EC broth followed by differential identification on MacConkey sorbitol agar. The presumptive positive isolates were further confirmed by multiplex polymerase chain reaction (PCR) using primers specific to rfbE (O157), fliC (H7), VT1 (MK1), and VT2 (MK2). Vero-toxicity and antibiotic sensitivity were examined in PCR confirmed isolates. Results: Out of the 257 samples analyzed, 1.9% (2/105) were positive for O157:H7 and 39% (41/105) were positive for VTEC. Two PCR confirmed positive O157:H7 strains and two randomly selected PCR-positive VT strains exhibited in vitro cytopathic effect on Vero cells on day-7 post-inoculation. Antibiotic sensitivity profiling of O157:H7 strains exhibited resistance against penicillin G, kanamycin, ampicillin, tetracycline, gentamycin, cefotaxime, streptomycin, and piperacillin. Conclusion: These findings reveal the presence of pathogenic E. coli O157:H7 in the healthy cattle of Pune; in a situation, wherein regular surveillance for O157:H7 is not a norm. Therefore, the findings presented herein warrant routine surveillance and public awareness to prevent the transfer of such pathogens and manage health risks to the public.

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Prevalence of vero toxic Escherichia coli in fecal samples of domestic as well as wild ruminants in Mathura districts and Kanpur zoo

Cited by 4 publications

References 30 publications

A study on association of virulence determinants of verotoxic Escherichia coli isolated from cattle calves

A study on association of virulence determinants of verotoxic Escherichia coli isolated from cattle calves

Samsun ili ve ilçelerinde yetiştirilen Anadolu mandalarının dışkı örneklerinde Escherichia coli O157:H7’nin tespiti

Isolation and characterization of Escherichia coli serotype O157:H7 and other verotoxin-producing E. coli in healthy Indian cattle

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