Prevalence of virulence factors and antibiotic resistance in vancomycin-resistant Enterococcus faecium isolated from sewage and clinical samples in Iran

Jahangiri, Sahar; Talebi, Malihe; Eslami, Gita; Pourshafie, Mohammad Reza

doi:10.4103/0255-0857.71828

Cited by 14 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR is a more accurate technique in comparison to the biochemical approach, so PCR results were preferred for the strains with discrepant identification. 22 In this study, the esp gene was detected in 78.3% of E. faecalis and 82% of E. faecium isolates, this finding is similar to the results of other studies, which identified the esp gene in 47.1%, 23 73%, 24 68.4% 7 of E. faecalis , and 80%, 25 26 65%, 27 66%, 23 71%, 24 75% 12 of E. faecium strains. However, this is in contrast to the findings of Shankar et al 28 and Channaiah et al 29 that reported the absence of esp in E. faecium .…”

Section: Discussionsupporting

confidence: 91%

“…In some studies, asa 1 was not found in E. faecium but in contrast, in some studies this gene was detected in lower frequency and in our study and some other studies this gene was identified in higher prevalence among E. faecium isolates (Comerlato et al 38 and Kowalska-Krochmal et al 39 ), were detected it among 5%, 65% of VRE fm and 2.7%, 60% of VRE fs strains, respectively. Jahangiri et al 12 were not found asa 1 gene in either 49 of VRE fm strains or 17 of VSE fm strains. Sharifi et al 13 were detected asa 1 gene in 80% of VRE fs and 7.89% of VRE fm strains.…”

Section: Discussionmentioning

confidence: 87%

“…In the study by Sauer et al 32 the esp gene was identified in 62.9% and 46.3% of VRE and VSE strains, respectively. Also, in the study by Jahangiri et al 12 esp gene was identified in 82% of clinical VRE fm isolates and 53% of VSE fm isolates. The esp and hyl genes have significantly a higher prevalence among ampicillin-resistant VRE fm isolates (53.7%, 37.3%) than ampicillin-susceptible VRE fm isolates (19.4%, 22.4%); which is similar to the results of other studies.…”

Section: Discussionmentioning

confidence: 87%

“…Willems demonstrated that the esp gene is a marker of the high prevalence of Enterococcus strains resistant to vancomycin in hospitalized patients, 30 but according to the study of Woodford et al, 31 Sauer et al, 32 and Jahangiri et al 12 the esp gene also was identified in sensitive-vancomycin strains. In some studies, the prevalence of gene esp is reversed among VRE fm and VSE fm strains.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Correlation between Infective Factors and Antibiotic Resistance inEnterococciClinical Isolates in West of Iran

2017

View full text Add to dashboard Cite

The present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of Enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 E. faecalis and 67 E. faecium isolated from clinical specimens were used. The isolates were identified, and then antibiotic susceptibility testing was performed. The MIC of vancomycin and teicoplanin were determined by broth microdilution method. The presence of infective genes esp, hyl and asa1 was scrutinized using PCR. Of the 280 enterococcal isolates, 175 (62.5%) isolates were identified as E. faecalis, 67 (24%) as E. faecium and 38 (13.5%) as Enterococcus spp. The results of the antibiotic susceptibility testing showed resistance rates of 5% and 73% to vancomycin and teicoplanin in E. faecalis and E. faecium isolates, respectively. The statistical analysis showed that the esp infective gene has significant associations with ciprofloxacin, erythromycin and tetracycline in E. faecium and with chloramphenicol in E. faecalis strains; the hyl with teicoplanin and vancomycin in E. faecium strains; and also asa1 with vancomycin in E. faecium and with ampicillin and chloramphenicol in E. faecalis strains. Regarding the relationships between virulence genes and antibiotic resistance in strains of E. faecalis and E. faecium, detection of infective factors associated with invasive diseases has become a major issue of concern.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Correlation between Infective Factors and Antibiotic Resistance inEnterococciClinical Isolates in West of Iran

2017

View full text Add to dashboard Cite

show abstract

“…In a study with the aim to determine the difference in virulence factors expressed by VRE and VSE, it was clearly found that factors like hemolysin production and biofilm formation were more in VSE than VRE isolates, though the difference was not statistically significant [ 6 ]. Similarly, in another study, it was observed that presence of hyl gene, along with simultaneous presence of hyl and esp genes and bacterial adhesion to Vero cells, was more in VSE than VRE isolates, both from clinical and environmental sources [ 32 ]. In a pilot study on enterococcal UTI from the same centre, VRE isolates possessed significantly fewer virulence factors than the susceptible isolates [ 33 ].…”

Section: Discussionmentioning

confidence: 94%

Prevalence of Virulence Factors and Drug Resistance in Clinical Isolates of Enterococci: A Study from North India

Banerjee

Anupurba

2015

Journal of Pathogens

View full text Add to dashboard Cite

Along with emergence of multidrug resistance, presence of several virulence factors in enterococci is an emerging concept. This study was undertaken to determine the prevalence of various virulence factors phenotypically and genotypically in enterococci and study their association with multidrug resistance. A total of 310 enterococcal isolates were studied, comprising 155 E. faecium and 155 E. faecalis. Antimicrobial susceptibility testing was done by disc diffusion and agar dilution method. Hemolysin, gelatinase, biofilm production, and haemagglutination were detected phenotypically and presence of virulence genes, namely, asa1, gelE, cylA, esp, and hyl, was detected by multiplex PCR. Of the total, 47.41% isolates were high level gentamicin resistant (HLGRE) and 7.09% were vancomycin resistant (VRE). All the virulence traits studied were found in varying proportions, with majority in E. faecalis (p > 0.05). Strong biofilm producers possessed either asa1 or gelE gene. gelE silent gene was detected in 41.37% (12/29). However, increase in resistance was associated with significant decrease in expression or acquisition of virulence genes. Further, acquisition of vancomycin resistance was the significant factor responsible for the loss of virulence traits. Though it is presumed that increased drug resistance correlates with increased virulence, acquisition of vancomycin resistance might be responsible for reduced expression of virulence traits to meet the “biological cost” relating to VRE.

show abstract

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolated from Intensive Care Units

2014

View full text Add to dashboard Cite

To assess the molecular characterization of disseminated vancomycin-resistant enterococci (VRE) in the intensive care units, 546 enterococci isolates were collected from different clinical samples in a prospective observational study. The results showed that a total number of 33 isolates (6 %) were resistant to vancomycin. Most of the VRE isolates 11 (34 %) were isolated from intensive care units (ICUs). 18 (55 %) VRE isolates were obtained from urinary tract infections. The results from pulsed-field gel electrophoresis showed five common types (CT) and 13 single types (ST) among the VRE isolates. The analysis showed two and one major CTs and ST among the ICUs isolates, respectively. Tn1546 transposon was analyzed using ClaI-digested long PCR (L-PCR) RFLP followed by sequencing. The results showed the presence of two different lineages of transposon among the two clonal groups. Lineage 1 with the arrangement of Tn1546 prototype in the first clonal group and the second lineage with 13 kb harboring two insertion sequences, IS1216 V and IS1542. DNA hybridization showed that vanA gene in all VRE isolates, with an exception of one isolate, was present in the same location on the genome. Overall, the results suggest that a few VRE clonal types were disseminated in ICUs in hospitals in Iran which were able to transfer their vanA with high conjugation frequency.

show abstract

Prevalence of virulence factors and antibiotic resistance in vancomycin-resistant Enterococcus faecium isolated from sewage and clinical samples in Iran

Abstract: Our results demonstrated that higher number of the clinical E. faecium isolates carried virulence genes than the isolates from STP. Finally, the lack of the genes in clinical and STP isolates confirmed that these genes do not transfer horizontally.

Cited by 14 publications

References 21 publications

Correlation between Infective Factors and Antibiotic Resistance inEnterococciClinical Isolates in West of Iran

Correlation between Infective Factors and Antibiotic Resistance inEnterococciClinical Isolates in West of Iran

Prevalence of Virulence Factors and Drug Resistance in Clinical Isolates of Enterococci: A Study from North India

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolated from Intensive Care Units

Contact Info

Product

Resources

About