Prevalence of VP4 and VP7 genotypes of human rotavirus in Ecuadorian children with acute diarrhea

Naranjo, Alfredo; Cedeño, Cecilia; Terán, Enrique; Castello, Alejandro

doi:10.1002/jmv.21181

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…A recent study in Ecuador has reported G9 at only 43% among samples collected in 10 clinics during 2006 (Naranjo et al, 2008) and our data show that in 2007, G9 prevalence fell to below 10% (unpublished data). A rapid decline was also recently reported in Thailand, where G9 rotavirus peaked at >90% prevalence in 2001–02, but accounted for less than a third of typed samples in the two following years (Khamrin et al, 2007).…”

Section: Introductionsupporting

confidence: 70%

Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus

et al. 2009

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Rotavirus is the most common cause of acute gastroenteritis among infants and young children throughout the world, but rotavirus cases in developing countries account for nearly all of the ∼600,000 annual deaths. We studied the epidemiology of rotavirus in 22 rural communities in northern coastal Ecuador over a five-year period. From 250 rotavirus positive stool specimens, the percentage that could not be RT-PCR genotyped for VP4 and VP7 was 77% and 63%, respectively. The possibility of sample degradation was considered but discounted after an experimental examination of rotavirus stability and EM visualization of rotavirus-like particles in several untypeable samples. Finally, alternate primers were used to amplify Ecu534, a sample that was untypeable using most published VP4 and VP7 primers. Characterization of the VP7, VP4, and VP6 full gene segments revealed novel genotypes and nucleotide mismatches with most published primer sequences. When considered with other findings, our results suggest that primer mismatch may be a widespread cause of genotyping failure, and might be particularly problematic in countries with greater rotavirus diversity. The novel sequences described in this study have been given GenBank accession numbers EU805775 (VP7), EU805773 (VP4), EU805774 (VP6) and the RCWG has assigned them novel genotypes G20P[28]I13, respectively.

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Section: Introductionsupporting

confidence: 70%

Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus

et al. 2009

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“…In a previous publication, data was presented showing that in 2005 G9 was the dominant strain circulating along the coast of Esmeraldas and in the Andean city of Quito [Endara et al, 2007]. This was consistent with another study describing rotavirus in urban settings in Ecuador [Naranjo et al, 2008]. There have been many other studies corroborating the emergence of G9 rotavirus in Latin America [Gentsch et al, 2005;.…”

Section: Discussionsupporting

confidence: 82%

“…Two studies describing the rotavirus genotype distribution were conducted during 2005 in Ecuador [Endara et al, 2007;Naranjo et al, 2008]. Both studies showed that G9 was the most common genotype circulating throughout the country (10 provinces within the coastal, Andian, and Amazonian regions).…”

Section: Introductionmentioning

confidence: 99%

Rapid changes in rotaviral genotypes in Ecuador

Hasing

Trueba

Baquero

et al. 2009

Journal of Medical Virology

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Previous studies suggest that the emerging G9P [8] genotype was the most prevalent rotavirus genotype in Ecuador during 2005. This present study provides a temporal analysis of the distribution of rotavirus genotypes in two locations within Ecuador by adding additional years (2006 -early 2008) to the originally reported 2005 data. Data were collected in a rural (northern coastal Ecuador) and urban (Quito) area. In the rural area, a community sample of cases (those presenting diarrhea) and controls (those not presenting diarrhea) were collected between August 2003 and March 2008 resulting in a total of 3,300 stool samples (876 cases and 2,424 controls). Of these samples, 260 were positive for rotavirus by an immunochromatographic test (196 cases and 64 controls). In Quito, 59 fecal samples were collected from children presenting diarrhea and diagnosed with rotavirus. An RT-PCR analysis of samples collected between 2005 and 2007 suggested that G9 was replaced by G1 and G2 in the rural and urban settings. During this period G9 decreased from 79% to 9% while G2 increased from 0% to 43% in the rural communities, and G9 decreased from 79% to 37% while G2 increased from 3% to 57% in the urban area of Quito. This rapid replacement of G9 by G1 and G2 reinforces the necessity of surveillance to inform vaccination programs.

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“…This pair of genotypes was detected in 41 out of 61 positive samples, which is quite a surprising result, as no single pair of genotypes has so far been observed to predominate [ 15 – 20 ]. Moreover, both the P9 and G3 genotype are rarely found in other studies: the G3 genotype is only seen in 1% to 5% of cases [ 16 – 18 , 20 ]. Only Ye et al .…”

Section: Discussionmentioning

confidence: 90%

Predominance of genotype P[9]G3 in rotavirus gastroenteritis in Polish children

Piekarska¹,

Kacerka²,

Majda-Stanisławska³

et al. 2015

aoms

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IntroductionRotavirus (RV) infection is the most common cause of gastroenteritis in children. This paper identifies the most common genotypes of rotaviruses isolated from children hospitalized with gastroenteritis and attempts to determine any relationship between infection with a certain rotavirus genotype.Material and methodsThe investigated group consisted of 68 consecutive children with rotavirus gastroenteritis (confirmed by an agglutination test). Rotavirus genotype was determined in stool samples obtained from each child.ResultsThe P[9]VP4 genotype was observed in 41/61 positive samples (over 67.2%) that were permanently associated with the G3 VP7 genotype. Moreover, G3 was determined as the most commonly isolated G type (77.94%). As well as the P[9]G3 type, G3 was also found in the P[4] type (5 cases). Twenty-six out of 61 (42.6%) children in whom rotavirus genotype was determined were co-infected with pathogenic bacteria. No statistical correlation was observed between rotavirus P[9]G3 gastroenteritis and digestive tract co-infection with pathogenic bacteria (p > 0.05). Elevated ALT activity was found in 34/59 (57.6%) cases of rotavirus gastroenteritis. Elevated ALT serum level was found to correlate with P[9]G3 rotavirus genotype but concomitant infections did not.ConclusionsThe most common genotype of rotaviruses observed in our group of children, P[9]G3, has rarely been described. Co-infection of the digestive tract with pathogenic bacteria and elevated serum ALT concentrations were found to be the most frequent phenomena. A correlation between P[9]G3 rotavirus genotype and elevated serum ALT level was found, but no significant relationship was identified between concomitant infections and P[9]G3 genotype.

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Prevalence of VP4 and VP7 genotypes of human rotavirus in Ecuadorian children with acute diarrhea

Cited by 9 publications

References 37 publications

Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus

Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus

Rapid changes in rotaviral genotypes in Ecuador

Predominance of genotype P[9]G3 in rotavirus gastroenteritis in Polish children

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