Prevalence, resistance pattern, and molecular characterization of Staphylococcus aureus isolates from healthy animals and sick populations in Henan Province, China

Abstract

BackgroundStaphylococcus aureus is one of the most prevalent pathogens and a causative agent of a variety of infections in humans and animals. A total of 640 samples were collected from healthy animals and patients from 2013 to 2014 in Henan Province, China, to investigate the prevalence and perform molecular characterization of S. aureus. Antimicrobial resistance and virulence genes were determined and pulsed-field gel electrophoresis (PFGE) and staphylococcal cassette chromosome mec (SCCmec) typing were performed.ResultsOverall, 22.3% (n = 143) of the samples were positive for S. aureus. The prevalence of methicillin-resistant S. aureus (MRSA) was 5.59%. Capsular polysaccharide locus type 5 (Cap5; 56.64%) was the dominant serotype. S. aureus strains showed high resistance to penicillin (96.50%), ciprofloxacin (52.45%), amikacin (67.83%), erythromycin (96.50%), lincomycin (97.20%), and tetracycline (68.53%) and 109 (76.2%) isolates harbored six or more tested resistance genes. The most predominant resistance genes were aphA (52.45%), ermC (53.15%), and tetM (52.45%). Eighty-seven (60.8%) isolates harbored six or more tested virulence genes. The most predominant enterotoxin genes were sed (20.28%), sej (20.98%), sep (14.69%), and set (37.76%). The prevalence of lukED gene was (57.34%), and a small number of isolates carried pvl (5.59%) and TSST-1 (2.80%). A total of 130 (82.52%) isolates could be typed by PFGE with SmaI digestion. PFGE demonstrated that 45 different patterns (P) that were grouped into 17 pulsotypes and 28 separate pulsotypes using a 90% cut-off value. A total of 118 (82.52%) isolates were successfully typed by spa, and 26 spa types were identified, t15075 (14.00%) and t189 (12.59%) were the most common types. SCCmec types were detected from eight MRSA isolates, with the most prevalent type being SCCmec IVa. MRSA-SCCmec Iva-t437 was observed in human isolates.ConclusionThis study revealed a high prevalence of S. aureus in healthy animals and patients from Henan Province, China. Resistant S. aureus exhibited varying degrees of multidrug resistance. The presence of antibiotic resistance and virulence genes may facilitate the spread of S. aureus strains and pose a potential threat to public health, highlighting the need for vigilant monitoring of these isolates at the human–animal interface.Electronic supplementary materialThe online version of this article (10.1186/s13099-018-0254-9) contains supplementary material, which is available to authorized users.

In vitro activity of antimicrobial agents with and without sulbactam, EDTA and sulbactam-EDTA on ESBLs-producing Escherichia coli isolated from healthy Tibetan yaks

“…This study was supported by grants from the National Natural Science Foundation of China (No. 81973739), [1] the Henan Post-doctoral Research Project Start-up Funding (No. 19030075)…”

“…Nowadays, the growing frequency of antibiotic resistances is a universal problem. The emergence of multidrug resistant (MDR) strains poses several challenges to the clinical facilities [1]. The production of β-lactamases is still the main mechanism for resistance of gram-negative bacilli to β-lactams [2].…”

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

“…Staphylococcus aureus (S. aureus) is a versatile opportunistic pathogen exiting widely in humans and food producing animals, such as pigs, poultry, ruminants and rabbits [1,2]. Many human diseases, ranging from superficial skin and soft tissue infection, pneumonia, septicemia, to endocarditis and other severe or even fatal diseases, are associated with S. aureus [3].…”

“…Since then, the prevalence of LA-MRSA has been concerned and detected by many scientists in different animals and countries [1,[14][15][16][17]. Human infection might be transmitted from pig-associated MRSA, such as ST398 [18].…”

“…Although there have been a great deal of reports on the epidemiological studies of S. aureus originated from food producing animals in China in recent years [1,29,30], little is known about the prevalence and occurrence of this organism in Chongqing, China. In this study, we investigated the prevalence of S. aureus isolated from food animals in Chongqing.…”