Prevalencia de anticuerpos séricos frente a Bartonella spp. en una polación sana del área sur de la provincia de Sevilla

García‐García, José A.; Baquerizo, R.; Vargas, Julio; Mira, José A.; Merchante, Nicolás; Macı́as, Juan; Pineda, Juan A

doi:10.1016/s0014-2565(05)72634-5

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…Nevertheless, clinical descriptions [ 5 , 17 , 26 ] and seroepidemiological studies about the prevalence of B. henselae/B. quintana [ 27 – 29 ] have been reported. In our previous studies carried out in La Rioja (North of Spain), B. henselae seroprevalences in cat owners, HIV-infected patients and blood donors were 28.9%, 17.3% and 5.9%, respectively, using a commercial kit [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain

et al. 2017

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BackgroundThe genus Bartonella includes fastidious, facultative intracellular bacteria mainly transmitted by arthropods and distributed among mammalian reservoirs. Bartonella spp. implicated as etiological agents of zoonoses are increasing. Apart from the classical Bartonella henselae, B. bacilliformis or B. quintana, other species (B. elizabethae, B. rochalimae, B. vinsonii arupensis and B. v. berkhoffii, B. tamiae or B. koehlerae, among others) have also been associated with human and/or animal diseases. Laboratory techniques for diagnosis (culture, PCR assays and serology) usually show lack of sensitivity. Since 2005, a method based on a liquid enrichment Bartonella alphaproteobacteria growth medium (BAPGM) followed by PCRs for the amplification of Bartonella spp. has been developed. We aimed to assess culture, molecular and serological prevalence of Bartonella infections in companion animal veterinary personnel from Spain.MethodsEach of 89 participants completed a questionnaire. Immunofluorescence assays (IFA) using B. vinsonii berkhoffii (genotypes I, II and III), B. henselae, B. quintana and B. koehlerae as antigens were performed. A cut-off of 1:64 was selected as a seroreactivity titer. Blood samples were inoculated into BAPGM and subcultured onto blood agar plates. Bartonella spp. was detected using conventional and quantitative real-time PCR assays and DNA sequencing.ResultsAmong antigens corresponding to six Bartonella spp. or genotypes, the lowest seroreactivity was found against B. quintana (11.2%) and the highest, against B. v. berkhoffii genotype III (56%). A total of 27% of 89 individuals were not seroreactive to any test antigen. Bartonella spp. IFA seroreactivity was not associated with any clinical sign or symptom. DNA from Bartonella spp., including B. henselae (n = 2), B. v. berkhoffii genotypes I (n = 1) and III (n = 2), and B. quintana (n = 2) was detected in 7/89 veterinary personnel. PCR and DNA sequencing findings were not associated with clinical signs or symptoms. No co-infections were observed. One of the two B. henselae PCR-positive individuals was IFA seronegative to all tested antigens whereas the other one was not B. henselae seroreactive. The remaining PCR-positive individuals were seroreactive to multiple Bartonella spp. antigens.ConclusionsHigh serological and molecular prevalences of exposure to, or infection with, Bartonella spp. were found in companion animal veterinary personnel from Spain. More studies using BAPGM enrichment blood culture and PCR are needed to clarify the finding of Bartonella PCR-positive individuals lacking clinical symptoms.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2483-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain

et al. 2017

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“…Preliminary estimates of the prevalence of antibody to Bartonella among apparently healthy humans range from 5.88% to 24.7% [ 17 , 20 ]. In a study carried out on a healthy population sample from the same area (83 were men and 78 women, and the mean age with positive serology was 45.18 ± 14.26 years), the seroprevalence was 10.6% [ 21 ], a figure that is very similar to that reported in other studies.…”

Section: Discussionmentioning

confidence: 99%

“…Our study highlights in the first place an antibody seroprevalence of 22.4% to Bartonella species in HIV patients, which is superior to that observed in the healthy population [ 13 , 19 , 25 ], with the exception of that described in some other studies [ 25 ]. Some authors consider HIV patients as a risk group for Bartonella infection [ 34 , 35 ] (characteristic personal and hygienic habits); however, other authors do not consider this infection risk to be greater [ 20 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…57.6% of seropositive patients were intravenous drug users, these findings are most likely a result of several conditions that are frequent among IVDUs: living conditions with a high probability of close contact with bacteriaemic cats, fleas, lice, and other potential vectors, repeated parenteral exposures, insufficient medical care, and malnutrition [ 19 , 33 , 34 , 37 ]. Other studies do not conclude that drug use might play such an important role [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Bartonellaspp. infection in HIV patients in Catalonia, Spain

Pons

Sanfeliú²,

Nogueras

et al. 2008

BMC Infect Dis

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Background: Although the first clinical descriptions of Bartonella infection were associated with immunocompromised patient with bacillary angiomatosis, we currently know that this organism is directly involved in diseases affecting a large number of patients, regardless of their immune status. Cat scratch disease, hepatic peliosis, and some cases of bacteraemia and endocarditis, are directly caused by some species of the genus Bartonella. The purpose of this study was to determinate the prevalence of IgG antibodies against Bartonella henselae and B. quintana in HIV patients and to identify the epidemiological factors involved.

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“…The prevalence of IgG antibodies in a healthy population in Sevilla was 24.7%. These data arise that B. henseale infections are frequent in these areas and most of them subclinical 8 .…”

Section: Introductionmentioning

confidence: 84%

Incidencia de la infección por Bartonella henselae en la Comunidad Valenciana durante el período 2009-2012

Fernández-Arias

Borrás-Máñez

Colomina-Rodríguez

et al. 2015

Rev. Esp. Salud Publica

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Prevalencia de anticuerpos séricos frente a Bartonella spp. en una polación sana del área sur de la provincia de Sevilla

Cited by 10 publications

References 14 publications

Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain

Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain

Seroprevalence of Bartonellaspp. infection in HIV patients in Catalonia, Spain

Incidencia de la infección por Bartonella henselae en la Comunidad Valenciana durante el período 2009-2012

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