Prevalencia de Leptospirosis y su relación con la tasa de gestación en bovinos de la zona centro de Veracruz

Martínez, Juan Prisciliano Zárate; Fernández, Jorge Víctor Rosete; Utrera, Ángel Ríos; Piña, Francisco Tobías Barradas

doi:10.21640/ns.v7i14.112

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…The results of the risk model showed a moderate level of expected frequency of anti‐ Leptospira antibodies in cattle herds from the state of Veracruz (Figure 3), and such findings partially differed from those of a previous study (Zárate Martínez et al., 2015). These authors studied seven herds of the central zone of the state of Veracruz and obtained frequencies of 10%–89%.…”

Section: Discussioncontrasting

confidence: 70%

“…In Mexico, clinical cases of leptospirosis have been described in humans (Sanchez‐Montes et al., 2015; Zuñiga‐Carrasco & Caro‐Lozano, 2013), and the presence of bacteria or exposure to them through polymerase chain reaction or microscopic agglutination test (MAT) has been described in domestic (Carmona‐Gasca et al., 2011; Zárate‐Martínez et al., 2015) and wild animals (Ballados‐González et al., 2018; Espinosa‐Martinez et al., 2015; Gutiérrez‐Molina et al., 2019; Torres‐Castro et al., 2018, 2020). Human cases, as the presence of Leptospira in domestic and wild animals, are more frequent in the south‐eastern states of the country, which include Tabasco, Yucatan and Veracruz (Ballados‐González et al., 2018; Gutiérrez‐Molina et al., 2019; Torres‐Castro et al., 2018, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Spatial epidemiology ofLeptospirasp. exposure in bovines from Veracruz, México

Gutiérrez-Molina

Acevedo

Sánchez‐Montes

et al. 2021

Transbounding Emerging Dis

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Bovine leptospirosis is a bacterial disease that affects cattle herds, causing economic losses due to reproductive problems which require expensive treatments. The main source of transmission for cattle is still uncertain, but rodents and bats can play an important role in the transmission cycle by being maintenance hosts for the pathogenic species of the bacterium and spreading it through urine. In this study, we characterize possible risk areas for bovine leptospirosis exposure in the state of Veracruz, Mexico, based on the geographical distribution of flying (bats) and terrestrial (rodents and opossums) wild hosts of Leptospira sp. reported in Mexico, in addition to climate, geography, soil characteristics, land use and human activities (environmental variables). We used a generalized linear regression model to understand the association between the frequency of anti-Leptospira sp. antibodies (a proxy of exposure) in cattle herds exposed to Leptospira, the favourability of wild hosts of Leptospira as well as the environmental variables. The parameterized model explained 12.3% of the variance. The frequency of anti-Leptospira sp. antibodies exposure in cattle herds was associated with elevation, geographic longitude, pH of the soil surface and environmental favourability for the presence of rodents, opossums and bats. The variation in exposure was mainly explained by a longitudinal gradient (6.4% of the variance) and the favourability-based indices for wild hosts (9.6% of the variance). Describing the possible risks for exposure to Leptospira in an important and neglected livestock geographical region, we provide valuable information for the selection of areas for diagnosis and prevention of this relevant disease.

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Section: Discussioncontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Spatial epidemiology ofLeptospirasp. exposure in bovines from Veracruz, México

Gutiérrez-Molina

Acevedo

Sánchez‐Montes

et al. 2021

Transbounding Emerging Dis

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“…During January to September 2016, we realized several collection journeys in three localities of Veracruz, Mexico, an state with previous records of bovine leptospirosis cases (Velasco‐Osorio, ; Zárate‐Martínez et al., ) (Figure ). This study was approved by the Ethics and Research Committee of the Medical Faculty of the UNAM ( Universidad Nacional Autónoma de México ) (FMED/CI/JMO/004/2012).…”

Section: Methodsmentioning

confidence: 99%

“…Although leptospirosis is a human and veterinary problem in Mexico, especially in states with tropic climate such as Tabasco and Veracruz with a human incidence that fluctuates between 4.39 and 11.74 cases per 100,000 inhabitants (Sánchez‐Montes, Espinosa‐Martínez, Ríos‐Muñoz, Berzunza‐Cruz, & Becker, ) and a seroprevalence in cattle that varies from 60% to 80% in Veracruz (Zárate‐Martínez, Rosete‐Fernández, Ríos‐Utrera, Barradas‐Piña, & Olazarán‐Jenkins, ), few studies have been carried out in order to identify wild hosts involved in the transmission of this pathogenic spirochaetes, all of them concentrated in terrestrial (Cantu et al., ; Espinosa‐Martínez et al., ; López‐Pérez et al., ; Méndez et al., ; Montiel‐Arteaga, Atilano, Ayanegui, Ceballos, & Suzán, ; Ruiz‐Pina, Puc‐Franco, Flores‐Abuxapqui, Vado‐Solis, & Cardenas‐Marrufo, ; Vado‐Solís et al., ) or marine mammals (Acevedo‐Whitehouse et al., ; Aragón‐Martínez, Olivera‐Gómez, & Jiménez‐Domínguez, ; Avalos‐Téllez et al., ; Godínez et al., ; Ziehl‐Quirós, García‐Aguilar, & Mellink, ), with a lack of knowledge regarding bats as potential reservoirs of these bacteria. To identify the role of bats as carriers of pathogenic leptospires, we detected and evaluated the diversity of Leptospira using molecular techniques in samples of kidneys from several bat species of Veracruz, Mexico.…”

Section: Introductionmentioning

confidence: 99%

Detection of pathogenicLeptospiraspecies associated with phyllostomid bats (Mammalia: Chiroptera) from Veracruz, Mexico

Ballados‐González

Sánchez‐Montes

Romero-Salas

et al. 2018

Transbound Emerg Dis

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The genus Leptospira encompass 22 species of spirochaetes, with ten pathogenic species that have been recorded in more than 160 mammals worldwide. In the last two decades, the numbers of records of these agents associated with bats have increased exponentially, particularly in America. Although order Chiroptera represents the second most diverse order of mammals in Mexico, and leptospirosis represents a human and veterinary problem in the country, few studies have been conducted to identify potential wildlife reservoirs. The aim of this study was to detect the presence and diversity of Leptospira sp. in communities of bats in an endemic state of leptospirosis in Mexico. During January to September 2016, 81 bats of ten species from three localities of Veracruz, Mexico, were collected with mist nets. Kidney samples were obtained from all specimens. For the detection of Leptospira sp., we amplified several genes using specific primers. Amplicons of the expected size were submitted to sequencing, and sequences recovered were compared with those of reference deposited in GenBank using the BLAST tool. To identify their phylogenetic position, we realized a reconstruction using maximum-likelihood (ML) method. Twenty-five samples from three bat species (Artibeus lituratus, Choeroniscus godmani and Desmodus rotundus) showed the presence of Leptospira DNA. Sequences recovered were close to Leptospira noguchii, Leptospira weilii and Leptospira interrogans. Our results include the first record of Leptospira in bats from Mexico and exhibit a high diversity of these pathogens circulating in the state. Due to the finding of a large number of positive wild animals, it is necessary to implement a surveillance system in populations of the positive bats as well as in related species, in order to understand their role as carriers of this bacterial genus.

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“…However, there are other states of México where human and bovine leptospirosis is a major public health issue. Specifically, the state of Veracruz records an incidence of human leptospirosis of five cases per 100,000 inhabitants, and seroprevalence in livestock ranging between 60 % and 80 % (Moles-Cervantes et al 2002;Álvarez et al 2005;Sánchez-Montes et al 2015;Zárate-Martínez et al 2015). However, there is no evidence on the Leptospira species maintained in rodent populations in the region.…”

Section: Resultsmentioning

confidence: 99%

Molecular evidence for the presence of Leptospira borgpetersenii in synanthropic rodents in the Nautla region, Veracruz, México

Gutiérrez-Molina

Cruz‐Romero

Romero-Salas

et al. 2019

Therya

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The genus Leptospira encompasses ten species of spirochetes capable of infecting mammals, particularly rodents. In México, studies focused on the detection of Leptospira sp. in rodents are scarce, all of them restricted to three states of the Gulf of México. For this reason, this work aimed to identify the diversity of Leptospira species associated with synanthropic rodents in Veracruz, a state where leptospirosis is endemic. Rodents were sampled with Sherman traps placed in 10 Production Units across the Nautla region. Animals were euthanized and their kidneys removed. Subsequently, a 474-bp segment of the outer membrane protein LipL32, present in all pathogenic species, was amplified and sequenced. Sequences were compared vs. reference using the BLAST algorithm: a phylogenetic reconstruction was carried out using the Maximum Likelihood method. In addition, the prevalence of infection in each Production Unit was estimated. Twenty eight rodents of a single species (Mus musculus) were caught. Leptospira DNA was detected in 17 samples (62.9 %, CI 95 % 42.3 to 80.6) from seven localities in the Nautla region. The sequences recovered exhibited 99-100% identity to each other and 99 % identity with Leptospira borgspetersenii sequences deposited in GenBank. This study confirms the presence of L. borgspetersenii in rodents, particularly in M. musculus, in México. This study increases the inventory of pathogenic leptospires for the state of Veracruz to three species.El género Leptospira engloba 10 especies de espiroquetas capaces de infectar mamíferos, particularmente roedores. En México se han realizado escasos estudios para la detección de Leptospira sp. en roedores, todos ellos restringidos a tres estados del Golfo de México. Por tal motivo el objetivo del presente trabajo fue identificar la diversidad de leptospiras en roedores sinantrópicas de Veracruz, un estado endémico de leptospirosis. Para la colecta de roedores, se colocaron trampas tipo Sherman en 10 unidades de producción de la región Nautla. Los animales se sacrificaron y se obtuvieron los riñones. Posteriormente se amplificó y secuenció un segmento de 474 pb de la proteína exterior de membrana LipL32 presente en las leptospiras patógenas. Posteriormente se compararon las secuencias con las de referencia mediante el uso del algoritmo BLAST y se realizó una reconstrucción filogenética mediante el método de Máxima Verosimilitud. Adicionalmente se obtuvieron las prevalencias de la infección por unidad de producción. Se colectaron 28 roedores de una única especie (Mus musculus). Se detectó la presencia de ADN de Leptospira en 17 muestras (62.9 %; IC 95 % 42.3 a 80.6) procedentes de siete localidades de la región Nautla. Las secuencias recuperadas exhibieron una similitud del 99-100 % entre si y una identidad del 99 % con secuencias de referencia de Leptospira borgspetersenii depositadas en GenBank. Este estudio confirma la presencia de L. borgspetersenii en roedores y en particular con M. musculus en México. Este estudio incrementa a tres especies el inventario ...

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Prevalencia de Leptospirosis y su relación con la tasa de gestación en bovinos de la zona centro de Veracruz

Cited by 5 publications

References 3 publications

Spatial epidemiology ofLeptospirasp. exposure in bovines from Veracruz, México

Spatial epidemiology ofLeptospirasp. exposure in bovines from Veracruz, México

Detection of pathogenicLeptospiraspecies associated with phyllostomid bats (Mammalia: Chiroptera) from Veracruz, Mexico

Molecular evidence for the presence of Leptospira borgpetersenii in synanthropic rodents in the Nautla region, Veracruz, México

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