Prevention of oral biofilm formation and degradation of biofilm by recombinant α-1,3-glucanases from <i>Streptomyces thermodiastaticus</i> HF3-3

Abstract

Prevention of oral biofilm formation and degradation of biofilm by recombinant α α α α α-1,3-glucanases from Streptomyces thermodiastaticus HF3-3

α-1,3-Glucanase from the gram-negative bacterium Flavobacterium sp. EK-14 hydrolyzes fungal cell wall α-1,3-glucan

“…Agl-EK14 was stable over a wide pH range and inactive on substrates except for α-1,3-glucan. These properties were similar to those of α-1,3-glucanases investigated for removing dental plaque, such as MutP from Paenibacillus curdlanolyticus MP-1 44 , Agl-ST1 and Agl-ST2 from S. thermodiastaticus HF3-3 45 , and mutanase RM1 from Paenibacillus sp. RM1 46 .…”

Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms

“…The production of recombinant biofilm-degrading enzymes has been attempted in microbes such as Escherichia coli ( Dobrynina et al, 2015 ; Cherdvorapong et al, 2020 ), but this is challenging because the inherent antibacterial properties of these products can interfere with host cell growth ( Oey et al, 2009 ). Plants such as Nicotiana benthamiana and tobacco ( Nicotiana tabacum ) are promising alternative hosts that benefit from inexpensive and rapidly scalable upstream production as well as product yields of more than 4 g kg −1 fresh leaf biomass ( Yamamoto et al, 2018 ) combined with high biomass yields of ∼100,000 kg ha −1 y −1 ( N. tabacum ) ( Stoger et al, 2002 ; Buyel et al, 2017 )].…”

Effect of mutanase and dextranase on biofilms of cariogenic bacteria: A systematic review of in vitro studies

“…The studies were published from 2004 to 2023, and evaluated the effect of dextranase (23 studies) [ [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] ], mutanase (ten studies) [ 25 , 39 , 44 , [48] , [49] , [50] , [51] , [52] , [53] , [54] ], or combined mutanase and dextranase treatment (seven studies) [ 25 , 39 , 44 , [55] , [56] , [57] , [58] ] on biofilm formation. Mutanase was obtained from fungi in six studies [ 44 , 48 , 53 , 55 , 57 , 58 ] and from bacteria in six studies [ 25 , 49 , [50] , [51] , [52] , [56] ], while fungal and bacterial dextranase were used in 12 [ 31 , 32 , 37 , 38 , 40 , 44 , 46 , 47 , [55] , [56] , [57] , ...…”

“… Author, year Enzyme Enzyme source Enzyme concentration Outcome Treatment Control treatment Biofilm model; growth medium Surface Methods for biofilm quantification e Main results for biofilm inhibition (percent reduction) Main results for biofilm removal (percent reduction) Bem, 2023 [ 48 ] Mutanase Fungus Trichoderma harzianum NR Biofilm removal Pre-formed 72 h-old biofilms enzyme-treated 2x/day (1 min) for 2 days Saline; inactivated enzyme S. mutans; TYEB with 0.1 μM glucose Glass (slide) CFU NA No significant differences between enzyme group and the controls (values: NR). Boddapati, 2023 [ 49 ] Mutanase Bacterium Cellulosimicrobium funkei SNG1 2.3 U/mL Biofilm removal Pre-formed 72 h-old biofilms enzyme-treated for 2 h No treatment a S. mutans; BHI with 5 % sucrose (w/v) Glass (coverslip) A 550 NA 82.7 % Cherdvorapong, 2020 [ 50 ] Mutanase Bacterium Streptomyces thermodiastaticus HF3-3 0.01 U/mL Biofilm inhibition; Biofilm removal Biofilms grown in the presence of mutanase for 4–16 h; Pre-formed 16 h-old biofilms enzyme-treated for 4–16 h Enzyme buffer S. mutans ; BHI with 1 % sucrose (w/v) Glass (plate) Alcian blue staining 12 h: 65–79 %; 16 h: ∼50 % 8 h: 60 %; 16 h: 40 % ...…”