Prevention of Oxidative Stress Injury to Sperm

Agarwal, Ashok; Prabakaran, Sushil; Said, Tamer M.

doi:10.2164/jandrol.05016

Cited by 254 publications

(167 citation statements)

References 86 publications

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“…The shift in the balance between oxidants and antioxidants in favor of oxidants is termed "oxidative stress." [18].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the protective and treatment effects of vinpocetine in myocardial infarctional with isoprotenol in rats

Güven¹,

Sarıhan²,

Parlakpınar³

et al. 2017

Med-Science

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As aim of this study, the knowledge of whether vinpocetine (VINPO) is cardioprotective or not following isoprotenol (ISO)-induced cardiac ischemia in rats. In myocard infarction, the one of the responsible mechanisms of injury is oxidative damage and inflammmation. The effect of VINPO which is the potent antioxidant and anti inflammatory agent aimed to reveal as the biochemical, electrophysiological, and histopathologic parameters. In this study, thirty-two Wistar-Albino male rats were divided into four groups, each consisting of eight rats. The group 1 named as sham, no any drug used in this group. Group 2 named as iso group, only ISO was adminestered, the group 3 named as VINPO and iso group, here initially vinpocetine then ISO were used, and the group 4 named as iso and VINPO, here initially ISO then VINPO were used. For the rats in group 2, in first and second day ISO adminestered at a dose of 120mg/kg using an intraperitoneal injection. At third and fourth day no any drug used. And at fifth day the experiment terminated. For the rats in group 3, at the first and second day VINPO adminestered at adose of 20 mg/kg using an intraperitoneal (ip) injection, after 30 minute isoprotenol administered at adose of 120 mg/kg using an ip injection. At third and fourth day no any drug was used. And at fifth day experiment was terminated. Lastly for the rats in group4, first and second day ISO administered at a dose of 120 mg/kg using an ip injection, at third and fourth day VINPO adminestered at a dose of 20 mg/kg using an ip injection, and at fifth day experiment terminated. Prior to termination of experiment the pulse rate and ECG changes were recorded. After completion of experiment protocols blood samples and cardiac tissue samples were recieved. For the knowledge of effects of VINPO experimental miyocardial ischemia induced in rats, and the serum myoglobulin, total creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanin aminotransferase (ALT), measured. In myocardial tissue as an antioxidative system and an oxidative stres markers; malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathine (GSH), total oxidant status (TOS), total antioxidant status (TAS), oxidative stres index (OSI) were studied. As a result, VINPO showed positive impact on cardiac functions in the light of electrocardiographic, biochemical and histopathological. According to our results, it is possible to say that it exerts these beneficial effetc via its powerfull antioxidant, antiinflammatory and free radical scavenger effects.

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“…The shift in the balance between oxidants and antioxidants in favor of oxidants is termed "oxidative stress." [18].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the protective and treatment effects of vinpocetine in myocardial infarctional with isoprotenol in rats

Güven¹,

Sarıhan²,

Parlakpınar³

et al. 2017

Med-Science

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“…Spermatozoa contain a small amount of cytoplasm, and therefore are poor in antioxidant enzymes like superoxide dismutase, glutathione peroxidase, and catalase. In addition, residual sperm cytoplasm is concentrated at the center of spermatozoa, which makes it unlikely for any antioxidant to protect spermatozoal membrane from head to tail [72,73].…”

Section: Free Radical Theory Of Aging and Apoptosismentioning

confidence: 99%

Effects of aging on the male reproductive system

Güneş

Hekim

Arslan

et al. 2016

J Assist Reprod Genet

195

118

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The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life, as more couples choose to postpone having a child due to various socioeconomic reasons, research for understanding the effects of aging on the reproductive system has gained an increased importance. Paternal aging also causes genetic and epigenetic changes in spermatozoa, which impair male reproductive functions through their adverse effects on sperm quality and count as, well as, on sexual organs and the hypothalamic-pituitarygonadal axis. Hormone production, spermatogenesis, and testes undergo changes as a man ages. These small changes lead to decrease in both the quality and quantity of spermatozoa. The offspring of older fathers show high prevalence of genetic abnormalities, childhood cancers, and several neuropsychiatric disorders. In addition, the latest advances in assisted reproductive techniques give older men a chance to have a child even with poor semen parameters. Further studies should investigate the onset of gonadal senesce and its effects on aging men.

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“…This ROS causes npg lipid peroxidation of the sperm plasma membrane, which has detrimental effects (i.e. decreased progressive motility and acrosomal integrity, increased mid-piece abnormalities and inhibited sperm-oocyte fusion) on spermatozoa during cryopreservation [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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Prevention of Oxidative Stress Injury to Sperm

Cited by 254 publications

References 86 publications

Investigation of the protective and treatment effects of vinpocetine in myocardial infarctional with isoprotenol in rats

Investigation of the protective and treatment effects of vinpocetine in myocardial infarctional with isoprotenol in rats

Effects of aging on the male reproductive system

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

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