Prevention of the rise in plasma cholesterol and glucose levels by kaki‐tannin and characterization of its bile acid binding capacity

Abstract

BACKGROUND Bile acid‐binding agents, such as cholestyramine and colesevelam, improve both cholesterol and glucose metabolism. Kaki‐tannin, a polymerized condensed tannin derived from persimmon (Diospyros kaki), has been shown to have bile acid‐binding capacity and a hypocholesterolemic effect. However, its effects on glucose metabolism have not been well studied, and the binding selectivity of kaki‐tannin to bile acid molecules has not been reported. RESULTS In vivo experiments using mice with high‐fat diet‐induced obesity showed that kaki‐tannin intake (20 g kg−1 of the diet) increased fecal bile acid excretion by 2.3‐fold and prevented a rise in plasma cholesterol levels and fasting plasma glucose levels. Kaki‐tannin also suppressed the development of impaired glucose tolerance. To characterize the bile acid‐binding capacity of kaki‐tannin, we investigated its capacity to bind to eight types of bile acid and cholesterol in vitro. Kaki‐tannin showed strong capacity to bind to lithocholic acid (85.5%), which has one hydroxy group. It also showed moderate capacity to bind to bile acids with two hydroxy groups (53.3%), followed by those with three hydroxy groups (39.0%), but kaki‐tannin did not show binding capacity to cholesterol. These results suggest that the binding capacity of kaki‐tannin to bile acids tends to decrease as the number of hydroxy groups increases. Interestingly, the binding capacity of kaki‐tannin correlated with that of cholestyramine (correlation coefficient: r = 0.900). CONCLUSION Our findings indicate that kaki‐tannin binds preferentially to bile acids with fewer hydroxy groups and has beneficial effects on glucose metabolism as well as cholesterol metabolism. © 2020 Society of Chemical Industry

In vitro antioxidant, anti-glycation, and bile acid-lowering capacity of peanut milk fermented with Lactiplantibacillus plantarum Kinko-SU4

“…Therefore, studying food materials with antioxidant, anti-glycation, and bile acid-lowering properties is important. ( Nishida et al, 2021 ; Vijaykrishnaraj and Wang, 2021 ; Mirmiran et al, 2022 ).…”

Dietary Phenolic Compounds: Their Health Benefits and Association with the Gut Microbiota

“…Kaki tannin has the property of binding with bile acids [ 87 ] and the effect of lowering cholesterol and ameliorating glucose metabolism [ 88 , 89 ]. Kaki tannins have also been reported to reshape the gut microbiota in rats fed a high-cholesterol diet [ 90 ].…”

The Effect of Lithocholic Acid on the Gut-Liver Axis

“…Supplementation of natural products may also affect the excretion of LCA. Kaki-tannin (an extract from persimmon) preferentially binds to monohydroxy LCA and promotes its excretion ( Nishida et al, 2021 ). Ethanolic extract of Fructus schisandrae enhances the excretion of BAs to the intestine and feces and restores the structure of intestinal flora that is destroyed by excess LCA ( Li et al, 2020 ).…”

“…Glycyrrhizin and oleanolic are found to accelerate LCA detoxification by up-regulating the expression of detoxification enzymes ( Wang et al, 2012 ; Chai et al, 2015 ). Kaki-tannin increases the fecal excretion of LCA ( Nishida et al, 2021 ). Bufalin enhances the activation of vitamin D receptor (VDR) via LCA binding ( Nakano et al, 2005 ).…”