Preventive effect of Lactobacillus plantarum HFY15 on carbon tetrachloride (CCl₄)‐induced acute liver injury in mice

Abstract

Carbon tetrachloride (CCl 4 ) is the main chemical causing liver damage. In this experiment, the effect of Lactobacillus plantarum HFY15 treatment on CCl 4 -induced acute liver injury was investigated using mice. Fifty adult mice were randomized into five study groups, each group with 10 ml kg -1 saline, 50 mg kg -1 silymarin, and 10 9 CFU kg -1 L. plantarum HFY15 and LDSB per day, and all the mice expect the normal group were injected 0.8% CCl 4 (10 ml kg -1 ) on the 14th day. Following the 16 h induction of the liver injury, various biochemical markers were assessed for blood and liver tissue. After L. plantarum HFY15 treatment, the content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), malondialdehyde (MDA), and reactive oxygen species (ROS) in serum decreased by 67.7%, 65.0%, 41.9%, 59.5%, and 51.5%, respectively, and the level of antioxidant enzymes (total superoxide dismutation [T-SOD], catalase [CAT], glutathione [GSH]) increased by more than twofold. Pro-inflammatory cytokine interleukin-6 (IL-6), interferon-γ (INF-γ), and tumor necrosis factorα (TNF-α) decreased by more than 45% in serum and live. What is more, L. plantarum HFY15 increased the expression of antiapoptosis genes Bcl-2 by eightfold, inhibiting the expression of proapoptotic genes Caspase-3 and Bax by about threefold. Lactobacillus plantarum HFY15 has obvious protective effects on CCl 4induced liver injury by inhibiting oxidation, reducing the release of inflammatory factors, and exerting suppressive effect on apoptotic process in the CCl 4 -induced liver injury. Lactobacillus plantarum HFY15 can be developed as edible lactic acid bacteria for preventing liver toxicity.

Cyanidin Alleviated CCl4-Induced Acute Liver Injury by Regulating the Nrf2 and NF-κB Signaling Pathways

“…The radicals also impair the antioxidant defense system in vivo by depleting reducing agents and inhibiting antioxidant enzymes production. These effects contribute to oxidative stress and induce cell damage and death [ 23 ]. Oxidative stress also triggers activation of the NF-κB signaling pathway, resulting in neutrophil infiltration and subsequent inflammatory injury to the liver [ 24 ].…”

Recent Perspective of Lactobacillus in Reducing Oxidative Stress to Prevent Disease

“…Lactobacillus strains prevent other types of liver damage: In an animal model of carbon tetrachloride (CCl4)-induced liver fibrosis, L. plantarum HFY15 was found to alleviate liver injury through antioxidant, anti-inflammatory, and anti-apoptotic pathways [ 120 ]. In addition, selenium-enriched probiotics (SP) might also influence pro- and anti-apoptosis pathways, activate the SIRT1 signaling pathway, or attenuate MAPK signaling, leading to reduced liver fibrosis [ 121 , 122 ].…”

Pleurospermum candollei Methanolic Extract Ameliorates CCl4-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

“…These toxic substances and drugs are responsible for almost 50% of acute liver damages. It is well recognized that the combination of mechanisms including apoptosis, oxidative stress, and inflammation plays a role in the development of acute liver injury. , During aerobic respiration in the mitochondrial membrane through the electron transport chain, reactive oxygen species (ROS) are constantly generated as byproducts. Liver is very rich in mitochondria and has a high rate of oxygen consumption during metabolic processes, which increases its exposure to ROS …”