Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice

Zhu, Kai; Peng, Peng; Wu, Ning; Zhou, Xianrong; Mu, Jianfei

doi:10.1155/2020/5462836

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Then, 1 μL 10.0 μM upstream primer and 1 μL 10.0 μM downstream primer (Table 1 , Thermo Fisher Scientific) and 7.0 μL sterile double distilled water were added to the 1.0 μL reaction fluid for amplification. The reaction conditions were 95 °C denaturation for 3 min, 60 °C annealing for 30 s, and 95 °C extension for 1 s. The relative expression intensity of each gene to be tested and the control cell (hydrogen peroxide induced oxidative damage cell) was calculated by the 2 -∆∆Ct method through the measured CT value [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…According to the experimental operation of MDLF on oxidative damage induced by H 2 O 2 in HEK 293 T cells, the cells were cultured and treated with MDLF for 48 h. Then the MDA, SOD, GSH, GSH-Px, and CAT levels in cells of each group were measured according to the kit instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing City, China) [24].…”

Section: Determination Of Mda Sod Gsh Gsh Px and Cat Levels In Hementioning

confidence: 99%

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The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

Fang

et al. 2020

BMC Complement Med Ther

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Background In this study, Malus doumeri leaf flavonoids (MDLF) were used as the research object to observe their in vitro antioxidant stress ability. Hydrogen peroxide (H2O2) was used to induce oxidative stress in 293 T cells. Methods MTT, flow cytometry, and qPCR were used to verify the effect of MDLF. Results In vitro cell experiments showed that at a concentration of 0–160 μg/mL, MDLF did not affect the normal proliferation of human embryonic kidney 293 T cells (HEK 293 T cells), and MDLF had no cytotoxic effect in this concentration range. It was found that MDLF could maintain the survival of HEK 293 T cells (82.6%) at a high concentration (160 μg/mL). Morphological observation also found that MDLF can inhibit the cell structure imperfection caused by H2O2. It was also observed that MDLF could significantly increase the levels of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) and reduce the level of malondialdehyde (MDA). The results of quantitative polymerase chain reaction (qPCR) showed that MDLF could significantly up-regulate the mRNA expression levels of CAT, SOD, GSH, GSH-Px, B-cell lymphoma-2 (Bcl-2) and downregulate the expression levels of B-cell lymphoma-2 associated x protein (Bax), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa-B (NF-κB) in oxidative stress-injured cells. The HPLC analysis showed that MDLF contained hyperin, isoquercetin, quercitrin, hesperidin, myricetin, baicalin and quercetin. Conclusion From the experimental results, it was observed that MDLF has a strong anti-oxidation ability in vitro, and it can interfere with the oxidative stress damage caused by H2O2 in 293 T cells. Therefore, MDLF is a type of natural substance with good anti-oxidant effect, and it has the potential to interfere with many diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Mda Sod Gsh Gsh Px and Cat Levels In Hementioning

confidence: 99%

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

Fang

et al. 2020

BMC Complement Med Ther

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“…As a result, the aromatic free radicals formed will be stable owing to the resonance effect. On the other hand, polyphenols can enter the lipid bilayer of the gastric mucosa, protecting the entire lipid layer from oxidation [53,54]. Our results indicated that EPE has a gastroprotective effect against gastric ulcers ethanol-induced.…”

Section: Discussionmentioning

confidence: 65%

Evaluation of antioxidant and anti-ulcerogenic effects of Eremurus persicus (Jaub & Spach) Boiss leaf hydroalcoholic extract on ethanol-induced gastric ulcer in rats

2021

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This study aimed to investigate the antioxidant and protective effect of Eremurus persicus (Jaub & Spach) leaf hydroalcoholic extract (EPE) in preventing gastric ulcers induced by ethanol in rats. Wistar rats weighing 180-220 g were randomly divided into ve groups, included normal group, ethanol consumption group, ranitidine treatment experienced group, the recipient rats prepared by dose of 250 mg/kg plant extract, and the last group recipient the dose of 500 mg/kg plant extract. The gastric ulcer was induced by feeding 1 ml of 96% ethanol to each animal. The gastric ulcer index (UI), pH, oxidative stress parameters, and histopathological changes in all stomachs were measured. Pre-treatment of ethanol-induced rats with the EPE reduced (P<0.05) the ulcer index and gastric juice pH, compared to ethanol intake rats. Additionally, pre-treatment with EPE at a dose-dependent manner alleviated the gastric oxidative stress injury in rats by increasing CAT activity, tissue NO • and GSH levels. EPE also was able to decrease the levels of ROS, MDA, PCO and serum NO • . According to the results, it can be concluded that pre-treatment with EPE prevents the formation of gastric ulcers caused by ethanol, which can be attributed to the antioxidant activity of plant polyphenols compounds.

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“…LBT polyphenols activated the expression of copper/zinc SOD (Cu/Zn SOD), CAT, and neuronal nitric oxide synthase (nNOS) and downregulated the expression of iNOS and cyclooxygenase‐2 (COX‐2), thus protecting gastric mucosa and inhibiting gastric injury. The identical components of raw LBT and LBT polyphenols included GA, catechin, caffeine, EGCG, epicatechin, and epicatechin gallate, the independent action and synergistic effect of which have not been explored (Qian et al., 2018; Zhu, Peng et al., 2020). LBIT polyphenols also showed a protective effect against HCl/ethanol‐induced gastric damage by effectively elevating the serum levels of SOD, GSH, MDA, and secreted immunoglobulin A antibodies in the gastric.…”

Section: Bioactivitiesmentioning

confidence: 99%

A systemic review on Liubao tea: A time‐honored dark tea with distinctive raw materials, process techniques, chemical profiles, and biological activities

Feng,

Chen,

Song

et al. 2023

Comp Rev Food Sci Food Safe

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Liubao tea (LBT) is a unique microbial‐fermented tea that boasts a long consumption history spanning 1500 years. Through a specific post‐fermentation process, LBT crafted from local tea cultivars in Liubao town Guangxi acquires four distinct traits, namely, vibrant redness, thickness, aging aroma, and purity. The intricate transformations that occur during post‐fermentation involve oxidation, degradation, methylation, glycosylation, and so forth, laying the substance foundation for the distinctive sensory traits. Additionally, LBT contains multitudinous bioactive compounds, such as ellagic acid, catechins, polysaccharides, and theabrownins, which contributes to the diverse modulation abilities on oxidative stress, metabolic syndromes, organic damage, and microbiota flora. However, research on LBT is currently scattered, and there is an urgent need for a systematical recapitulation of the manufacturing process, the dominant microorganisms during fermentation, the dynamic chemical alterations, the sensory traits, and the underlying health benefits. In this review, current research progresses on the peculiar tea varieties, the traditional and modern process technologies, the substance basis of sensory traits, and the latent bioactivities of LBT were comprehensively summarized. Furthermore, the present challenges and deficiencies that hinder the development of LBT, and the possible orientations and future perspectives were thoroughly discussed. By far, the productivity and quality of LBT remain restricted due to the reliance on labor and experience, as well as the incomplete understanding of the intricate interactions and underlying mechanisms involved in processing, organoleptic quality, and bioactivities. Consequently, further research is urgently warranted to address these gaps.

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Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice

Cited by 6 publications

References 24 publications

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

Evaluation of antioxidant and anti-ulcerogenic effects of Eremurus persicus (Jaub & Spach) Boiss leaf hydroalcoholic extract on ethanol-induced gastric ulcer in rats

A systemic review on Liubao tea: A time‐honored dark tea with distinctive raw materials, process techniques, chemical profiles, and biological activities

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