Preventive Effects of Bee Pollen Cistus ladaniferus Extract on Bone Loss in Streptozotocin-Diabetic Rats In Vivo

Yamaguchi, Masayoshi; Hamamoto, Reiko; Uchiyama, Satoshi; Ishiyama, Kaori; Hashimoto, Ken

doi:10.1248/jhs.53.190

Cited by 27 publications

(18 citation statements)

References 33 publications

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“…Gayathri and Kannabiran (2008) showed that in STZ-diabetic rats, there was a significant increase in the serum Na, K and Ca. Uchiyama and Yamaguchi (2003) and Yamaguchi et al (2007) demonstrated that the levels of serum Ca were increased in STZ-diabetic rats. Yanardag et al (2005) and Ozsoy-Sacan et al (2006) reported that in the STZ-diabetic rats, the blood Na and K levels were statistically increased.…”

Section: Discussionmentioning

confidence: 99%

Physiological Study on the Effect of Acalypha wilkesiana Leaves Extract on Streptozotocin-Induced Experimental Diabetes in Male Mice

Al-Attar¹

2010

American Medical Journal

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Problem statement: Diabetes mellitus is a chronic disease that causes serious health complications and it is now one of the most common non-communicable diseases globally. It is the fourth or fifth leading cause of death in most high-income countries and there is substantial evidence that it is epidemic in many economically developing and newly industrialized nations. Recently, there has been a growing interest in antidiabetic agents from natural products especially those derived from plants. The antidiabetic agents have been focused on plants used in traditional medicine because that may be a better treatment than currently used synthetic drugs. Approach: The influence of Acalypha wilkesiana leaves extract on the levels of serum glucose, triglycerides, cholesterol, sodium (Na), potassium (K) and Calcium (Ca) was investigated using Streptozotocin (STZ)-induced diabetic mice. Diabetes was induced in male albino mice by single intraperitoneal injection of STZ (60 mg kg −1 body weight). The experimental animals were divided into four groups.The first group was untreated and served as normal control. The mice of second group were used as diabetic control group. The diabetic mice of third group were treated orally with A. wilkesiana leaves extract for 30 days. The normal mice of fourth group were treated orally with A. wilkesiana leaves extract at the same dose given to third group. Results: The diabetic mice showed significant increases in the levels of serum glucose, triglycerides, cholesterol and Na after 10, 20 and 30 days. Also, the level of K was statistically increased after 20 and 30 days, while the level of Ca was significantly elevated after 30 days. The diabetic mice given A. wilkesiana leaves extract showed significant decreases in the levels of serum glucose, cholesterol and K. Insignificant alterations in the values of serum triglycerides, Na and Ca were noted in diabetic mice treated with leaves extract at all periods of experiment. Conclusion/Recommendations: These new data indicate that A. wilkesiana represents an effective antihyperglycemic, antihyperlipidemic adjunct and ameliorative role on electrolytes disturbances for the treatment of diabetes and a potential source of discovery of new therapeutic agent.

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Section: Discussionmentioning

confidence: 99%

Physiological Study on the Effect of Acalypha wilkesiana Leaves Extract on Streptozotocin-Induced Experimental Diabetes in Male Mice

Al-Attar¹

2010

American Medical Journal

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“…The intake of cistus extract has preventive effects on bone loss in diabetic state and ovariectomized rats, 13,14) suggesting a role in the prevention of osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

“…10) Royal jelly does not have an inhibitory effect on osteoclastogenesis in mouse marrow culture in vitro, 12) so that the effect of bee pollen on bone metabolism may thus be unique among beerelated products. The intake of bee pollen cistus extract has been demonstrated to have preventive effects on bone loss induced in the diabetic state 13) and ovariectomized rats, 14) suggesting a useful role in the prevention of osteoporosis with aging.…”

Section: Introductionmentioning

confidence: 99%

Anabolic Effects of Bee Pollen Cistus ladaniferus Extract in Osteoblastic MC3T3-E1 Cells In Vitro

Yamaguchi

Uchiyama

Nakagawa

2007

JOURNAL OF HEALTH SCIENCE

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The effect of bee pollen extract on osteoblastic MC3T3-E1 cells was investigated. The watersolubilized extracts, which were obtained from the bee pollen of Cistus ladaniferus, was purified using the membrane fractionation method with molecular weight (MW) less than 1000. Osteoblastic cells were cultured for 72 hr in a medium containing either vehicle or cistus extract of less than MW 1000 (10, 25, or 50 µg/ml of medium) in the presence of 10% fetal bovine serum (FBS). The proliferation of osteoblastic cells was significantly enhanced in the presence of cistus extract (25 or 50 µg/ml). The stimulatory effect of cistus extract on cell proliferation was also observed when the cells with subconfluency were cultured for 24 or 72 hr without FBS. Cells with subconfluency were cultured for 24 or 72 hr in a medium containing either vehicle or cistus extract without FBS. DNA content or alkaline phosphatase activity in osteoblastic cells was significantly increased after culture with cistus extract (50 µg/ml) for 24 hr. The effect of 25 µg/ml of cistus extract on these components was seen after culture for 72 hr. This study demonstrates that the cistus extract fraction of less than MW 1000 has anabolic effects in osteoblastic MC3T3-E1 cells which involve in bone formation.

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“…duce bone loss, 22) has been shown to have preventive effects on bone loss with diabetes in vivo. 20) We examined the preventive effect of cistus extract on bone loss in OVX rats, a model of osteoporosis, in vivo, furthermore.…”

Section: Discussionmentioning

confidence: 99%

“…19) The intake of bee pollen cistus extract has been shown to have preventive effects on bone loss in streptozotocin-diabetic rats, and the intake has partial restorative effects on serum biochemical findings with the diabetic state in vivo. 20) This study was undertaken to determine whether the intake of bee pollen cistus extract has preventive effects on bone loss induced in ovariectomized (OVX) rats, a model of osteoporosis, in vivo.…”

Section: Introductionmentioning

confidence: 99%

Preventive Effects of Bee Pollen Cistus ladaniferus Extract on Bone Loss in Ovariectomized Rats In Vivo

Yamaguchi

Uchiyama

Nakagawa

2007

JOURNAL OF HEALTH SCIENCE

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The effect of bee pollen Cistus ladaniferus extract on ovariectomy (OVX)-induced bone loss in vivo was investigated. The water-solubilized extracts were obtained from the bee pollen of Cistus ladaniferus. Cistus extract (5.0 or 10.0 mg/100 g body weight) was orally administered once daily for 30 days to OVX rats. The analysis using a peripheral quantitative computed tomography (pQCT) showed that OVX-induced a significant decrease in mineral content, mineral density, and polar strength strain index in the femoral-metaphyseal tissues. These decreases were significantly prevented after the administration of cistus extract (10.0 mg/100 g). Moreover, OVX-induced a significant decrease in calcium content in the femoral-diaphyseal and -metaphyseal tissues. This decrease was significantly prevented after the administration of cistus extract (5.0 or 10.0 mg/100 g). This study demonstrates that cistus extract has a preventive effect on OVX-induced bone loss in vivo.

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Preventive Effects of Bee Pollen Cistus ladaniferus Extract on Bone Loss in Streptozotocin-Diabetic Rats In Vivo

Cited by 27 publications

References 33 publications

Physiological Study on the Effect of Acalypha wilkesiana Leaves Extract on Streptozotocin-Induced Experimental Diabetes in Male Mice

Physiological Study on the Effect of Acalypha wilkesiana Leaves Extract on Streptozotocin-Induced Experimental Diabetes in Male Mice

Anabolic Effects of Bee Pollen Cistus ladaniferus Extract in Osteoblastic MC3T3-E1 Cells In Vitro

Preventive Effects of Bee Pollen Cistus ladaniferus Extract on Bone Loss in Ovariectomized Rats In Vivo

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