Preventive effects of <i>Ophiocordyceps sinensis</i> mycelium on the liver fibrosis induced by thioacetamide

Wu, Yi-Hsieng Samuel; Tseng, Jung-Kai; Chou, Chung‐Che; Chiu, Chin‐Yi; Lin, Yi-Ling; Chen, Yi‐Chen

doi:10.1002/tox.22402

Cited by 12 publications

(15 citation statements)

References 35 publications

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“…TAA‐induced hepatotoxicity was correlated with a pronounced alteration in serum liver biomarkers ALP, ALT, AST, Bilirubin, total protein, and albumin. The amount of biomarkers leak into the blood indicates the severity of liver damage 49,50 …”

Section: Resultsmentioning

confidence: 99%

“…The amount of biomarkers leak into the blood indicates the severity of liver damage. 49,50 The changes in serum liver biomarkers on treatment with TAA, SY, and M. elliptica are indicated in Figures 5 and 6. There were significant variations (p < .0001) between all treatment groups in the serum levels of ALP, ALT, and AST with F (4, 25) values; 3469, 3473, and 3711 respectively.…”

Section: Analysis Of Liver Fibrosis Masson's Trichrome Stainingmentioning

confidence: 99%

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In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

Bradosty

Agha

Shaikh

et al. 2021

Environmental Toxicology

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Morinda elliptica L. (Rubiaceae) is a phytomedicinal herb, used to treat gastrointestinal complications in Peninsular Malaysia. The study evaluates the in vivo hepatoprotective activity of ethanolic extract of M. elliptica stem in thioacetamide (TAA) induced liver fibrosis in male Sprague Drawly rats. Thirty adult rats were divided into five groups of six rats each. Rats of the normal control group received intraperitoneal injections (i. p.) of vehicle 10% Tween-20, 5 ml/kg, and hepatotoxic group 200 mg/kg TAA three times per week respectively. Three supplementary groups were treated with TAA plus daily oral silymarin (50 mg/kg) or M. elliptica (250 or 500 mg/kg). After 8 weeks of treatment, all rats were sacrificed. Liver fibrosis was assessed by gross macroscopic and microscopic tissue analysis, histopathological, and biochemical analysis. The livers of the TAA treated group showed uniform coarse granules, hepatocytic necrosis with lymphocytes infiltration. Contrary, the livers of M. elliptica treated groups (250 and 500 mg/kg) were much smoother and the cell damage was much lesser. The livers of M. elliptica treated groups rats showed elevated activity of SOD and CAT with a significant decrease in MDA level at p < .0001. The level of liver damage parameters, that is, ALP, ALT, and AST, bilirubin, total protein, and albumin were restored to the normal comparable to silymarin. M. elliptica stem extract significantly promoted normal rat liver architecture with significant perfections in biochemical parameters. The molecular contents of M. elliptica with hepatoprotective influence could be discovered, is the future prospective of this study.

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Section: Resultsmentioning

confidence: 99%

Section: Analysis Of Liver Fibrosis Masson's Trichrome Stainingmentioning

confidence: 99%

In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

Bradosty

Agha

Shaikh

et al. 2021

Environmental Toxicology

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“…Co-treatment of CCH-As or silymarin lowered ( P < 0.05) serum ALT and ALP levels compared to the TAA group ( Table 3 ). This reversed effects on serum liver damage indices should result from the antioxidant effects of CCH-As or silymarin against TAA induction ( Wu et al., 2017 ; Yang et al., 2019 ). Lin et al.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of crude chalaza hydrolysates against liver fibrogenesis via antioxidation, anti-inflammation/anti-fibrogenesis, and apoptosis promotion of damaged hepatocytes

Lin

et al. 2021

Poultry Science

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Four-hundred metric-ton chalazae are produced annually from the liquid-egg processing and always cause a heavy burden due to handling cost in Taiwan. After chalazae were hydrolyzed by protease A, the amounts of hydrophobic, aromatic, and branched-chain amino acids, as well as anserine were dramatically increased. This study was to understand the antifibrogenic effects of protease A-digested crude chalaza hydrolysates ( CCH-As ) on livers of thioacetamide ( TAA ) treated rats. CCH-As improved ( P < 0.05) growth performance, serum liver damage indices, histopathological liver inflammation, and liver collagen deposition in TAA-treated rats. The antifibrogenic effects of CCH-As were due to decreased ( P < 0.05) inflammatory/fibrogenic cytokine contents, α-smooth-muscle-actin ( α-SMA ) protein expression, and matrix metallopeptidase ( MMP )-2 and -9 activities, as well as increased ( P < 0.05) the antioxidant capacity in livers. CCH-As also increased ( P < 0.05) cleaved caspase-3 and cleaved poly ADP-ribose polymerase protein levels in livers of TAA-treated rats which accelerating cell renewal. Thus, this study does not only reveal a novel nutraceutical ingredient, CCH-As, against liver fibrogenesis, but also offer an alternative way to expand the utilization of poultry byproducts.

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“…Paraffin-embedded liver sections were stained for F4/80 (anti-active macrophage) (Abcam, Cambridge, United Kingdom) and α-SMA (fibrosis hallmark) with immunohistochemistry (IHC) staining procedures as previously detailed[ 14 ]. Briefly, liver sections were incubated with a specific primary antibody, followed by incubation with horseradish peroxidase (HRP)-linked secondary antibody (Dako, Glostrup, Denmark) and 3,3’-diaminobenzidine; the sections were then scanned with the NanoZoomer Digital Pathology system.…”

Section: Methodsmentioning

confidence: 99%

Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis

et al. 2018

WJG

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AIMTo investigate changes in gut microbiota and metabolism during nonalcoholic steatohepatitis (NASH) development in mice fed a methionine-choline-deficient (MCD) diet.METHODSTwenty-four male C57BL/6J mice were equally divided into four groups and fed a methionine-choline-sufficient diet for 2 wk (Control 2w group, n = 6) or 4 wk (Control 4w group, n = 6) or the MCD diet for 2 wk (MCD 2w group, n = 6) or 4 wk (MCD 4w group, n = 6). Liver injury, fibrosis, and intestinal barrier function were evaluated after 2 and 4 wk of feeding. The fecal microbiome and metabolome were studied using 16s rRNA deep sequencing and gas chromatography-mass spectrometry.RESULTSThe mice fed the MCD diet presented with simple hepatic steatosis and slight intestinal barrier deterioration after 2 wk. After 4 wk of feeding with the MCD diet, however, the mice developed prominent NASH with liver fibrosis, and the intestinal barrier was more impaired. Compared with the control diet, the MCD diet induced gradual gut microbiota dysbiosis, as evidenced by a marked decrease in the abundance of Alistipes and the (Eubacterium) coprostanoligenes group (P < 0.001 and P < 0.05, respectively) and a significant increase in Ruminococcaceae UCG 014 abundance (P < 0.05) after 2 wk. At 4 wk, the MCD diet significantly reduced the promising probiotic Bifidobacterium levels and markedly promoted Bacteroides abundance (P < 0.05, and P < 0.01, respectively). The fecal metabolomic profile was also substantially altered by the MCD diet: At 2 wk, arachidic acid, hexadecane, palmitic acid, and tetracosane were selected as potential biomarkers that were significantly different in the corresponding control group, and at 4 wk, cholic acid, cholesterol, arachidic acid, tetracosane, and stearic acid were selected.CONCLUSIONThe MCD diet induced persistent alterations in the gut microbiota and metabolome.

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Preventive effects of Ophiocordyceps sinensis mycelium on the liver fibrosis induced by thioacetamide

Cited by 12 publications

References 35 publications

In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

In vivo hepatoprotective effect of Morinda elliptica stem extract against liver fibrosis induced by thioacetamide

Protective effects of crude chalaza hydrolysates against liver fibrogenesis via antioxidation, anti-inflammation/anti-fibrogenesis, and apoptosis promotion of damaged hepatocytes

Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis

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