Protective effects of crude chalaza hydrolysates against liver fibrogenesis via antioxidation, anti-inflammation/anti-fibrogenesis, and apoptosis promotion of damaged hepatocytes

Abstract

Four-hundred metric-ton chalazae are produced annually from the liquid-egg processing and always cause a heavy burden due to handling cost in Taiwan. After chalazae were hydrolyzed by protease A, the amounts of hydrophobic, aromatic, and branched-chain amino acids, as well as anserine were dramatically increased. This study was to understand the antifibrogenic effects of protease A-digested crude chalaza hydrolysates ( CCH-As ) on livers of thioacetamide ( TAA ) treated rats. CCH-As improved ( P < 0.05) growth performance, serum liver damage indices, histopathological liver inflammation, and liver collagen deposition in TAA-treated rats. The antifibrogenic effects of CCH-As were due to decreased ( P < 0.05) inflammatory/fibrogenic cytokine contents, α-smooth-muscle-actin ( α-SMA ) protein expression, and matrix metallopeptidase ( MMP )-2 and -9 activities, as well as increased ( P < 0.05) the antioxidant capacity in livers. CCH-As also increased ( P < 0.05) cleaved caspase-3 and cleaved poly ADP-ribose polymerase protein levels in livers of TAA-treated rats which accelerating cell renewal. Thus, this study does not only reveal a novel nutraceutical ingredient, CCH-As, against liver fibrogenesis, but also offer an alternative way to expand the utilization of poultry byproducts.

An alleviative effect of Lonicerae japonicae flos water extract against liver fibrogenesis in vitro and in vivo

“…The growth performance, serum biochemical values, proinflammatory cytokine content, oxidative stress status, and histological observation suggest that a dose of 100 mg TAA kg −1 BW 3 times a week for 8 weeks induces liver fibrogenesis in rats, similar to prior reports 2,18 . Rats intoxicated with TAA during liver fibrogenesis had larger liver weight or size and worse liver damage indices (higher AST, ALT, and ALP levels).…”

“…35 Our in vitro data suggest that the LJWES pretreatment can week for 8 weeks induces liver fibrogenesis in rats, similar to prior reports. 2,18 Rats intoxicated with TAA during liver fibrogenesis had larger liver weight or size and worse liver damage indices (higher AST, ALT, and ALP levels). These rats also had abnormal lipid metabolism in the liver, including reduced production and delivery efficiencies of apoprotein B and low-density lipoprotein and accumulation of microsomal sphingomyelin that alters membrane fluidity.…”

“…+ 2.5 mL kg −1 BW LJWES stock (oral gavage). The treatment of TAA (dose and injection frequency) was injected thrice weekly (Monday, Wednesday, and Friday) 2,17,18 . Chlorogenic acid and LJWES were orally given daily.…”

Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth

“…The findings here reported revealed among the medwakh smoker group an increased level of metabolites linked with inflammatory mechanism, such as pyroglutamic acid 23 , 24 , deoxyguanosine 25 and anserine 26 . In order to investigate futher an eventual association of metwakh smoking and inflammation, we decided to measure CRP, a widely known clinical indicator of inflammatory conditions 27 , 28 .…”

A functional chicken‐liver hydrolysate‐based supplement ameliorates alcohol liver disease via regulation of antioxidation, anti‐inflammation, and antiapoptosis

“…fertilizer or incineration. Recently, food-derived protein hydrolysates have been demonstrated to own several biofunctionalities like antioxidant, 4 cardioprotection, [5][6][7] hepatoprotection, [8][9][10] reproductive function, 11 and antihypertension 12 etc. It has been reported that biofunctionalities of protein hydrolysates or peptides mainly result from their amino acid composition or peptides with specific sequences and structures.…”