Preventive effects of pyrroloquinoline quinone on formation of cataract and decline of lenticular and hepatic glutathione of developing chick embryo after glucocorticoid treatment

Nishigori, Hideo; Yasunaga, Mika; Mizumura, Masashi; Lee, Jung W.; Iwatsuru, Motoharu

doi:10.1016/0024-3205(89)90044-1

Cited by 56 publications

(31 citation statements)

References 11 publications

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“…In vivo treatment of rabbits with the Dex eye drops, as used for cataract surgery, reduced GSH levels in the lens [63]. In addition, free radical scavengers have also been reported to prevent GC-induced cataract formation [64][65][66] by increasing GSH levels and decreasing LPO. Decreasing the levels of GSH, when reactive oxygen species are present, can trigger a cascade of further oxidative damage, such as LPO, which has been associated with the formation of cataracts in patients [67][68][69][70].…”

Section: Discussionmentioning

confidence: 99%

N-acetylcysteine amide protects against dexamethasone-induced cataract related changes in cultured rat lenses

Tobwala¹,

Pınarcı²,

Maddirala³

et al. 2014

ABC

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Glucocorticoids (GCs) are one of the most widely used immunosuppressive and anti-inflammatory agents. However, their long term and systemic use is associated with adverse drug reactions including posterior subcapsular cataracts as one of its ocular complications. Balanced redox state is crucial for maintenance of lens transparency, and a high content of glutathione (GSH) in the lens is believed to play a key role in doing so. Depletion of GSH is implicated in the etiopathogenesis of dexamethasone-induced cataracts and, therefore, the present study was sought to evaluate the efficacy of a novel thiol antioxidant, N-acetylcysteine amide (NACA), in preventing dexamethasone-induced cataractogenesis. Cataract formation was induced by incubation of rat lenses with 5 µM dexamethasone. To assess whether NACA had a significant impact on dexamethasone-induced cataracts, the rat lenses were divided into four groups: 1) control group (Dulbecco's Modified Eagle Medium (DMEM), 2) dexamethasone group (DMEM with 5 µM dexamethasone), 3) NACA-only group (50 µM NACA solution), and 4) NACA pretreatment group (50 µM NACA for 6 hours followed by 5 µM dexamethasone only for 18 hours). Lenses were cultured for 7 days at 37˚C under 5% CO 2 . Lenses were evaluated daily using a dissecting microscope and photographed and graded for the development of opacity. The rat lenses in both the control and the NACA-only groups were clear, whereas all lenses within the dexamethasone-only group developed well-defined cataracts. Overall observations indicated that NACA inhibits cataract formation by limiting lipid peroxidation and increasing the ratio of GSH/GSSG in lens. Therefore, NACA can be developed into a potential adjunctive therapeutic option for patients undergoing therapy with GCs to inhibit glucocorticoid-induced cataracts.

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Section: Discussionmentioning

confidence: 99%

N-acetylcysteine amide protects against dexamethasone-induced cataract related changes in cultured rat lenses

Tobwala¹,

Pınarcı²,

Maddirala³

et al. 2014

ABC

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“…Two papers regarding the protective effects of PQQ on chick embryo exposed to glucocorticoid treatment have been documented so far (Nishigori et al, 1989 and1993). However, information on efficacy of PQQ on traits of economic importance in broiler industry such as growth performance and carcass yield is scarce.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary pyrroloquinoline quinone disodium on growth performance, carcass yield and antioxidant status of broiler chicks

Samuel

Zhang

Wang

et al. 2015

Animal

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Pyrroloquinoline quinone (PQQ), a putative essential nutrient and redox modulator in microorganisms, cell and animal models, has been recognized as a growth promoter in rodents. Growth performance, carcass yield and antioxidant status were evaluated on broiler chickens fed different levels of PQQ disodium (PQQ.Na 2 ). A total of 784 day-old male Arbor Acres (AA) broilers were randomly allotted into seven dietary groups: negative control group (NC) fed a basal diet without virginiamycin (VIR) or PQQ.Na 2 ; a positive control group (PC) fed a diet with 15 mg of VIR/kg diet; and PQQ.Na 2 groups fed with 0.05, 0.10, 0.20, 0.40 or 0.80 mg PQQ.Na 2 /kg diet. Each treatment contained eight replicates with 14 birds each. The feeding trial lasted for 6 weeks. The results showed that chicks fed 0.2 mg PQQ.Na 2 /kg diet significantly improved growth performance comparable to those in PC group, and the feed efficiency enhancement effects of dietary PQQ.Na 2 was more apparent in grower phase. Dietary addition of PQQ.Na 2 had the potential to stimulate immune organs development, and low level dietary addition (<0.1 mg/kg) increased plasma lysozyme level. Broilers fed 0.2 mg PQQ.Na 2 /kg diet gained more carcasses at day 42, and had lower lipid peroxide malondialdehyde content and higher total antioxidant power in plasma. The results indicated that dietary PQQ.Na 2 (0.2 mg/kg diet) had the potential to act as a growth promoter comparable to antibiotic in broiler chicks.

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“…When 15-day-old chick embroys were treated with HC for 48 h almost all lenses became cataractous with stages IV-V as re ported previously [1,2,[4][5][6]. However, as shown in table 1, PGL, administered at the indicated time after HC administration, pre sented a dose-dependent effect against HCinduced cataract formation, with its triple ap plication (1.5 mmol/egg) at 3, 10 and 20 h showing the most significant preventive ef fect.…”

Section: Incidence O F Cataractous Lensesmentioning

confidence: 85%

“…Glucocorticoid (GC)-induced cataract for mation of developing chick embryos can be effectively prevented with tiopronin [1], as corbic acid [2] and its derivative [3], pyrroloquinoline quinone [4], SA3443 [5] and isoci trate [6], suggesting that the modification of GC-induced metabolic changes by radical over, the study reveals that a marked eleva tion of glucose in the lenses noted after GC administration [9,10] is not directly related to their opacification.…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid-lnduced Cataract of the Developing Chick Embryo – Prevention by Propylene Glycol

Nishigori¹,

Lee²,

Iwatsuru³

1995

Ophthalmic Res

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When 0.25 μmol of hydrocortisone succinate sodium (HC) was administered to 15-day-old hen’s fertile eggs, almost all lenses of the embryos became cataractous with stages IV-V ( > 90%) 48 h after the treatment. However, a triple application of propylene glycol (1.5 mmol/egg) at 3, 10 and 20 h after HC treatment effectively prevented the HC-induced cataract formation (I: 84%, II: 12%, III: 4%, IV-V: 0%) and repressed the decline of glutathione and the elevation of lipid peroxide in the lens caused by HC. Propylene glycol is metabolized to lactate and pyruvate producing NADH and is known to possess protective activities against X-ray irradiation. These properties have modified the HC-induced effects and decreased the production of oxidative stress in ovo, protecting the lens from losing its transparency after HC administration. Furthermore, a marked elevation of glucose in cataractous lenses noted after HC administration was not directly involved in opacification of the lenses.

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Preventive effects of pyrroloquinoline quinone on formation of cataract and decline of lenticular and hepatic glutathione of developing chick embryo after glucocorticoid treatment

Cited by 56 publications

References 11 publications

N-acetylcysteine amide protects against dexamethasone-induced cataract related changes in cultured rat lenses

N-acetylcysteine amide protects against dexamethasone-induced cataract related changes in cultured rat lenses

Effects of dietary pyrroloquinoline quinone disodium on growth performance, carcass yield and antioxidant status of broiler chicks

Glucocorticoid-lnduced Cataract of the Developing Chick Embryo – Prevention by Propylene Glycol

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