Preventive role of probiotic bacteria against gastrointestinal diseases in mice caused by Giardia lamblia

Al-Megrin, Wafa Abdullah I.; Mohamed, Shadia H.; Saleh, Moudy M.; Yehia, Hany M.

doi:10.1042/bsr20204114

Cited by 10 publications

(6 citation statements)

References 38 publications

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“…Whereas the antibacterial and antifungal activity [31,32] of L. acidophilus has been well illustrated, the antiparasitic properties of L. acidophilus have been less studied. Studies with mouse models of the diseases caused by Giardia lamblia [33], Toxocara canis [34], Trichinella spiralis [35], and Cryptosporidium parvum [36] reveal that a combination of probiotics and other probiotic strains is beneficial in the treatment and prevention of these parasites. In a recent study, we demonstrated that L. acidophilus is detrimental to E. histolytica but the amebicide mechanism was unknown [19].…”

Section: Resultsmentioning

confidence: 99%

Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics

Sarid

Zanditenas

et al. 2022

Antioxidants

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Amebiasis is an intestinal disease transmitted by the protist parasite, Entamoeba histolytica. Lactobacillus acidophilus is a common inhabitant of healthy human gut and a probiotic that has antimicrobial properties against a number of pathogenic bacteria, fungi, and parasites. The aim of this study was to investigate the amebicide activity of L. acidophilus and its mechanisms. For this purpose, E. histolytica and L. acidophilus were co-incubated and the parasite’s viability was determined by eosin dye exclusion. The level of ozidized proteins (OXs) in the parasite was determined by resin-assisted capture RAC (OX–RAC). Incubation with L. acidophilus for two hours reduced the viability of E. histolytica trophozoites by 50%. As a result of the interaction with catalase, an enzyme that degrades hydrogen peroxide (H2O2) to water and oxygen, this amebicide activity is lost, indicating that it is mediated by H2O2 produced by L. acidophilus. Redox proteomics shows that L. acidophilus triggers the oxidation of many essential amebic enzymes such as pyruvate: ferredoxin oxidoreductase, the lectin Gal/GalNAc, and cysteine proteases (CPs). Further, trophozoites of E. histolytica incubated with L. acidophilus show reduced binding to mammalian cells. These results support L. acidophilus as a prophylactic candidate against amebiasis.

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Section: Resultsmentioning

confidence: 99%

Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics

Sarid

Zanditenas

et al. 2022

Antioxidants

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“…Whereas the antibacterial and antifungal activity [33,34] of L. acidophilus has been well illustrated, the antiparasitic properties of L. acidophilus has been less studied. L. acidophilus in combination with other probiotics is beneficial in the prevention and treatment of Giardia lamblia infection in mice [35], Toxocara canis [36], Trichinella spiralis [37] and Cryptosporidium parvum [38]. In a recent work, we have demonstrated that L. acidophilus is detrimental to E. histolytica but the amebicide mechanism was unknown [19].…”

Section: Discussionmentioning

confidence: 99%

Insights Into the Mechanisms of Lactobacillus acidophilus Activity Against Entamoeba histolytica by Using Thiol-Redox-Proteomics

Sarid¹,

Zanditenas²,

Ye³

et al. 2022

Preprint

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Amoebiasis is an intestinal disease transmitted by the protist parasite, Entamoeba histolytica, following the ingestion of contaminated food and water. In the colon, E. histolytica can phagocytose bacteria that are the main components of the microbial flora. Most infected individuals are asymptomatic but for unknown reasons, the parasite can become virulent and invasive, causes amebic dysentery, and migrates to the liver, where they cause hepatocellular damage. For the last few decades, it has become evident that E. histolytica virulence is directly linked to its interaction with the gut microbiota. Lactobacillus acidophilus is a common inhabitant of healthy human gut and a probiotic that present antimicrobial activity against many pathogenic bacteria, fungi and parasites. The purpose of this study was to examine the mechanisms behind the innate amebicide activity of L.acidophilus. We found that this activity is mediated by hydrogen peroxide (H2O2) produced by L. acidophilus. Redox proteomics shows that L. acidophilus triggers the oxidation of many essential amebic enzymes like pyruvate:ferredoxin oxidoreductase, the lectin Gal/GalNAc and cysteine proteases (CPs). Further, trophozoites of E.histolytica cultivated with L.acidophilus show reduced binding to mammalian cells. These results support L.acidophilus as a prophylactic candidate against amoebiasis.

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“…These finding strengthen our understanding about the role of resident normal microflora of the intestine that can stimulate the pathogenicity of some intestinal protozoan. Beside normal microflora, probiotics have the potency to prevent evolution of some protozoan parasite; which will be discuss in the following section [61].…”

Section: Hypotheses Regarding Protozoan Pathogenic Stimulation By The...mentioning

confidence: 99%

“…Probiotics inhibits the advancement of certain intestinal pathogens [61,62]. Probiotics also effective and efficient in the supportive management of gastrointestinal disorders [61,62], infection based respiratory disease [63], and allergic symptoms [64], and "also can kill or inhibit or even kills strain-specific pathogens" [65] through several mechanisms, namely (1) Competition [66], (2) Molecule secretion [67], and/or (3) Immune induction [62].…”

Section: The Potency Of Probiotics Against Protozoan Parasitesmentioning

confidence: 99%

Intestinal Microflora Vs Protozoan Parasites: From Interaction to Competition

Siagian

2022

SAJRM

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Aims: To reveal the interaction between intestinal microflora and the protozoan parasite organisms and how it affect their host’s performance. Discussion: The vertebrate gastro-intestinal system contain wide array of diverse but dynamic bacterial microbiota population that has ubiquitous consequences on its host’s well-being including physiology, nutrition, metabolism, and immunity, locally and systematically. Naturally, these bacteria share their milleu with a more or less similar population of parasitic eukaryotes (e.g., protozoan, helminths, and fungi). Both eukaryotes parasites in combination with the prokaryotic microorganisms as inhabitant normal microflora can dynamically shift the bio-physics and immune milleu of the intestine (locally) or even can affect its host as a whole (systematically), creating abundant chances for them to interact to each other; where ideally, both side is in equilibrium state. Beside their function, intestinal normal (commensal) microflora mainly contribute in several activities that control parasite survival and determines the outcome of several, if not many, parasite-base disease. Normal microflora actively limiting the pathogenicity of many parasites. The steadiness among the number and composition of normal microflora and its host seems vital to the host’s well-being perpetuation. But unfortunately, this interaction can further shifted into competition that can leads to the dominance of one party in number and probably also strength. Those spectrum of interactions may critically modify infection outcomes (active or dormant/carrier) and in turn affect the overall host condition. Active protozoan invasion may modify interaction between hosts and their normal resident microflora, either supporting or preventing against the condition of dysbiosis and inflammatory disease. Conversely, the microbiota controls parasite's settlement, multiplication, and even virulency; the properties that can modulate the interaction along the parasitism-mutualism sphere. Conclusion: Intestinal microflora composition control the pathogenesis of the protozoan infections.

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Preventive role of probiotic bacteria against gastrointestinal diseases in mice caused by Giardia lamblia

Cited by 10 publications

References 38 publications

Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics

Insights into the Mechanisms of Lactobacillus acidophilus Activity against Entamoeba histolytica by Using Thiol Redox Proteomics

Insights Into the Mechanisms of <em>Lactobacillus acidophilus</em> Activity Against <em>Entamoeba histolytica</em> by Using Thiol-Redox-Proteomics

Intestinal Microflora Vs Protozoan Parasites: From Interaction to Competition

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