Preventive Vitamin A Supplementation Improves Striatal Function in 6-Hydroxydopamine Hemiparkinsonian Rats

Marie, Anaïs; Leroy, Julien; Darricau, Morgane; Alfos, Serge; Smedt-Peyrusse, Véronique de; Richard, Emmanuel; Vancassel, Sylvie; Bosch‐Bouju, Clémentine

doi:10.3389/fnut.2022.811843

Cited by 9 publications

(11 citation statements)

References 63 publications

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“…Scale bar: 0.5 mm for SN and 1 mm for striatum. (Lundblad et al, 2004;Marie et al, 2022;Mazzocchi et al, 2022). Here, we demonstrate that the intrastriatal injection of 4 and 8 µg 6-OHDA exerts spontaneous motor impairment, which partially recovered starting on Day 28 post-lesion.…”

Section: Discussionmentioning

confidence: 50%

“…Animal models of PD play an important role in the discovery of potential therapies and molecular mechanisms of the illness ( Dauer and Przedborski, 2003 ; Duty and Jenner, 2011 ; Im et al, 2016 ; Hamadjida et al, 2019 ; Salari and Bagheri, 2019 ; Rahman et al, 2020 ). The 6-OHDA striatal lesion model, more closely approximating early PD, has been commonly used for studying neuroprotective and neurotrophic strategies for PD, an active and important area of research ( Lundblad et al, 2004 ; Marie et al, 2022 ; Mazzocchi et al, 2022 ). Here, we demonstrate that the intrastriatal injection of 4 and 8 μg 6-OHDA exerts spontaneous motor impairment, which partially recovered starting on Day 28 post-lesion.…”

Section: Discussionmentioning

confidence: 99%

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Longitudinal assessment of motor function following the unilateral intrastriatal 6-hydroxydopamine lesion model in mice

Sun

Zhang

et al. 2022

Front. Behav. Neurosci.

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IntroductionDespite the widespread use of the unilateral striatal 6-hydroxydopamine (6-OHDA) lesion model in mice in recent years, the stability of behavioral deficits in the 6-OHDA striatal mouse model over time is not yet clear, raising concerns about using this model to evaluate a compound’s long-term therapeutic effects.Materials and methodsIn the current study, mice were tested at regular intervals in the cylinder test and gait analysis beginning 3 days after 6-OHDA injection of 4 and 8 μg and lasting until 56 days post-lesion. Apomorphine-induced rotational test and rotarod test were also performed on Day 23 and 43 post-lesion, respectively. Immunohistochemistry for dopaminergic neurons stained by tyrosine hydroxylase (TH) was also performed.ResultsOur results showed that both the 4 and 8 μg 6-OHDA lesion groups exhibited forelimb use asymmetry with a preference for the ipsilateral (injection) side on Day 3 and until Day 21 post-lesion, but did not show forelimb asymmetry on Day 28 to 56 post-lesion. The 8 μg 6-OHDA lesion group still exhibited forelimb asymmetry on Day 28 and 42 post-lesion, but not on Day 56. The gait analysis showed that the contralateral front and hind step cycles increased from Day 3 to 42 post-lesion and recovered on Day 56 post-lesion. In addition, our results displayed a dose-dependent reduction in TH+ cells and TH+ fibers, as well as dose-dependent apomorphine-induced rotations. In the rotarod test, the 8 μg 6-OHDA lesion group, but not the 4 μg group, decreased the latency to fall on the rotarod on Day 43 post-lesion.ConclusionIn summary, unilateral striatal 6-OHDA injections of 4 and 8 μg induced spontaneous motor impairment in mice, which partially recovered starting on Day 28 post-lesion. Forced motor deficits were observed in the 8 g 6-OHDA lesion group, which remained stable on Day 43 post-lesion. In addition, the rotarod test and apomorphine-induced rotational test can distinguish between lesions of different extents and are useful tools for the assessment of functional recovery in studies screening novel potential therapies.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Longitudinal assessment of motor function following the unilateral intrastriatal 6-hydroxydopamine lesion model in mice

Sun

Zhang

et al. 2022

Front. Behav. Neurosci.

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“…The findings of this investigation, which are presented in Table , clearly demonstrate the impact of isotretinion on the functioning enzymes in rats' livers (1) Numerous studies (9). some of which suggest that the level of serum AST, ALT and ALP remain within the normal range or only slightly increase during medication with low dose (10), and that was in agreement with the outcome of this study as it was clearly seen that there were no significant differences in the level of AST, ALT, and ALP of the low dose isotretinion.…”

Section: Discussionmentioning

confidence: 52%

Physiological and histological Study on liver of male albino rats treated with retinol drug (retain)

Abass

Al-Harbi

2022

ijhs

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Isotretinoin, commonly known as 13-cis-retinoic acid, is a vitamin derivative used to treat severe acne and several types of skin, head, and neck cancer. According to certain research, isotretinoin causes apoptosis in a number of different types of bodily cells. In instances of clinically obvious acute liver injury with jaundice, isotretinoin has not been definitively linked. It is unknown how isotretinoin elevates serum aminotransferase levels, however, given that this effect seems to occur more frequently with a greater dose of medication, it may be a direct harmful impact. Methods: This study was conducted in the animal house in the College of Science / University of Babylon for the period from 22/11/2021 to 22/12/2021. The study included 40 white male albino rats (Rattus rattus) aged 2-3 months, weighing 100 to 150 g, and at a temperature of (25+3C) and 12 u (light-dark cycle) and then divided into eight groups, each group containing five rats. The animals were left before treatment for two weeks to acclimatize. After that, the rats were treated with Isotretinoin and they were given free water and a ration.

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“…Cylinder test was used to assess spontaneous forelimb use during exploration of a confined and unfamiliar environment. Cylinder test was performed as previously described 27 . In a clear plexiglass cylinder (diameter: 16 cm; height: 31 cm), the number of touches with both forepaws on the cylinder wall while the rat was rearing was quantified during 3 min‐sessions 28 .…”

Section: Methodsmentioning

confidence: 99%

“…Cylinder test was performed as previously described. 27 In a clear plexiglass cylinder (diameter: 16 cm; height: 31 cm), the number of touches with both forepaws on the cylinder wall while the rat was rearing was quantified during 3 min-sessions. 28 Results were represented as the total number of touches made with both forepaws.…”

Section: Cylinder Testmentioning

confidence: 99%

Impact of dietary vitamin A on striatal function in adult rats

et al. 2023

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The striatum is a brain structure involved in the control of voluntary movement. Striatum contains high amounts of retinoic acid, the active metabolite of vitamin A, as well as retinoid receptors, RARβ and RXRγ. Previous studies revealed that disruption of retinoid signaling initiated during development is deleterious for striatal physiology and related motor functions. However, the alteration of retinoid signaling, and the importance of vitamin A supply during adulthood on striatal physiology and function has never been established. In the present study, we investigated the impact of vitamin A supply on striatal function. Adult Sprague–Dawley rats were fed with three specific diets, either sub‐deficient, sufficient, or enriched in vitamin A (0.4, 5, and 20 international units [IU] of retinol per g of diet, respectively) for 6 months. We first validated that vitamin A sub‐deficient diet in adult rats constitutes a physiological model of retinoid signaling reduction in the striatum. We then revealed subtle alterations of fine motor skills in sub‐deficient rats using a new behavioral apparatus specifically designed to test forepaw reach‐and‐grasp skills relying on striatal function. Finally, we showed using qPCR analysis and immunofluorescence that the striatal dopaminergic system per se was not affected by vitamin A sub‐deficiency at adult age. Rather, cholinergic synthesis in the striatum and μ‐opioid receptor expression in striosomes sub‐territories were the most affected by vitamin A sub‐deficiency starting at adulthood. Taken together these results revealed that retinoid signaling alteration at adulthood is associated with motor learning deficits together with discrete neurobiological alterations in the striatum.

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Preventive Vitamin A Supplementation Improves Striatal Function in 6-Hydroxydopamine Hemiparkinsonian Rats

Cited by 9 publications

References 63 publications

Longitudinal assessment of motor function following the unilateral intrastriatal 6-hydroxydopamine lesion model in mice

Longitudinal assessment of motor function following the unilateral intrastriatal 6-hydroxydopamine lesion model in mice

Physiological and histological Study on liver of male albino rats treated with retinol drug (retain)

Impact of dietary vitamin A on striatal function in adult rats

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