Prevotella in Pigs: The Positive and Negative Associations with Production and Health

Amat, Samat; Lantz, Hannah; Munyaka, Peris M.; Willing, Benjamin P.

doi:10.3390/microorganisms8101584

Cited by 85 publications

(70 citation statements)

References 130 publications

(215 reference statements)

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“…are common and important bacterial colonizers of human and porcine guts. 15,16 It has been shown that if species of the genera Prevotella and Bacteroides co-occur in the gut, one or the other predominates, suggesting antagonistic behavior. 16 Indeed, we observed that greater relative abundances of Prevotella were associated with fewer Bacteroides in pig workers and vice-versa in cattle workers.…”

Section: Discussionmentioning

confidence: 99%

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

et al. 2021

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It has been hypothesized that both genetics and diet influence the composition of the human cecal microbiota. However, it remains unclear whether and how occupational exposure to microbes impacts the microbial communities in human guts. Using a One Health approach, we visited pig farms (n = 26) and collected stool specimens from pig workers (n = 59), pig barn air samples (n = 19), and rectal swabs from pigs at three different growth stages (n = 144). Stool samples from cattle workers were included as a control group (n = 22). Each sample's microbiota was characterized using 16S rRNA gene sequencing and the DADA2 pipeline.We obtained a significantly different clustering of the microbial compositions of pig and cattle workers by permutational multivariate analysis of variance (PERMANOVA; P < .001). Workers primarily exposed to pigs had higher relative abundances of Prevotellaceae and less Bacteroidaceae than workers exposed to cattle. We also found that the microbial compositions of pig workers' stool samples shared extensive fractions with the samples from their pigs. We also identified amplicon sequencing variants (ASVs) in the airborne microbiota which were likely involved in zoonotic transmission events.We hypothesize that ASVs originating from pig feces are aerosolized and, through breathing, get trapped in the pig farm workers' upper respiratory tract from where they can get swallowed. Consequently, some of the animal associated ASVs are transferred into the gastrointestinal tracts (GITs) which leads to changes in the composition of the human gut microbiota. The importance of this finding for human health must be investigated further.

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Section: Discussionmentioning

confidence: 99%

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

et al. 2021

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“…Nevertheless, we were able to find some taxa associated with health, such as the Prevotellaceae family, which deserves further study. This family was also represented in the healthy farms at the ASVs level by Prevotella copri and Prevotella stercorea , two species previously found in higher abundance in suckling and weaning healthy piglets [ 18 ]. Two ASVs from the Lachnospiraceae family were also associated with healthy farms, in agreement with previous studies, where ASVs from this family were correlated with higher feed conversion ratio [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Altered Nasal Microbiota Composition Associated with Development of Polyserositis by Mycoplasma hyorhinis

et al. 2021

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Fibrinous polyserositis in swine farming is a common pathological finding in nursery animals. The differential diagnosis of this finding should include Glaesserella parasuis (aetiological agent of Glässer’s disease) and Mycoplasma hyorhinis, among others. These microorganisms are early colonizers of the upper respiratory tract of piglets. The composition of the nasal microbiota at weaning was shown to constitute a predisposing factor for the development of Glässer’s disease. Here, we unravel the role of the nasal microbiota in the subsequent systemic infection by M. hyorhinis, and the similarities and differences with Glässer’s disease. Nasal samples from farms with recurrent problems with polyserositis associated with M. hyorhinis (MH) or Glässer’s disease (GD) were included in this study, together with healthy control farms (HC). Nasal swabs were taken from piglets in MH farms at weaning, before the onset of the clinical outbreaks, and were submitted to 16S rRNA gene amplicon sequencing (V3–V4 region). These sequences were analyzed together with sequences from similar samples previously obtained in GD and HC farms. Animals from farms with disease (MH and GD) had a nasal microbiota with lower diversity than those from the HC farms. However, the composition of the nasal microbiota of the piglets from these disease farms was different, suggesting that divergent microbiota imbalances may predispose the animals to the two systemic infections. We also found variants of the pathogens that were associated with the farms with the corresponding disease, highlighting the importance of studying the microbiome at strain-level resolution.

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“…Negative correlations between animal body weight and abundance of Prevotellaceae have been described [80], while the Prevotelladominant enterotype has been associated with higher feed intake values compared to the Treponema-dominant enterotype in Duroc pigs [81]. As suggested by Amat et al [82], further analyses are required to clarify the role of specific species of Prevotella in pig performance. Within this family, Prevotella copri is the most abundant species found in pig gut microbiota after weaning.…”

Section: Microbiota Composition and Community Diversity Associated With MIX Supplementationmentioning

confidence: 93%

Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets

Dell’Anno

Reggi

Caprarulo

et al. 2021

Animals

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The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (p < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased Prevotella and Fibrobacter genera presence (p < 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (p < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota.

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Prevotella in Pigs: The Positive and Negative Associations with Production and Health

Cited by 85 publications

References 130 publications

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

Altered Nasal Microbiota Composition Associated with Development of Polyserositis by Mycoplasma hyorhinis

Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets

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