Primary Alcohols, Substrates for Phospholipase D-Catalyzed Transphosphatidylation, Suppress the Cytokinin Action

Romanov, G. A.; Getman, Irina A.; Bolyakina, Yu. P.; Rakova, Natalia Yu.; Kieber, Joseph J.; Schmülling, Thomas

doi:10.1007/978-94-017-2664-1_12

Cited by 4 publications

(6 citation statements)

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“…Such a rapid and mighty response was due to the control of this gene expression by the promoter of the cytokinin primary response ARR5 gene. Earlier, we have found that low concentrations of primary alcohols, 1-butanol in particular, suppressed the cytokinin-induced transcription of the ARR5 gene in Arabidopsis seedlings [6,7]. In agreement with these data, transcription of the reporter P ARR5 :GUS transgene was also reduced in the presence of only 0.5% 1-butanol approximately by two-thirds (Fig.…”

Section: Resultssupporting

confidence: 85%

“…Amaranthus and Arabidopsis seedlings responded to cytokinin (BA) by rapid specific reactions based on the activation of the primary response genes [5][6][7]. Cytokinin effect was assessed quantitatively in Amaranthus seedlings from the accumulation of the pigment amaranthin, whereas, in transgenic Arabidopsis seedlings, from the activity of the reporter GUS enzyme (see Materials and Methods section).…”

Section: Resultsmentioning

confidence: 99%

“…The basic pathway for the cytokinin signal transduction is believed to be phosphate transfer following the pattern His Asp His from sensor histidine kinase through specific proteins, phosphotransmitters, to intranuclear transcription factors. However, many important aspects of cytokinin signal transduction and also the way of interaction between this signal transduction pathway and other known signaling pathways remain obscure and under extensive study [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Polyamines suppress manifestation of cytokinin primary effects

Rakova

Romanov

2005

Russ J Plant Physiol

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Using Amaranthus and transgenic Arabidopsis P ARR5 : GUS seedlings in bioassays, we studied the effects of exogenous polyamines on the development of cytokinin-induced effects based on the expression of the cytokinin primary response genes. Transgenic Arabidopsis contained a reporter GUS gene under the control of Arabidopsis ARR5 promoter. In both bioassays, we found that all polyamines tested suppressed the effects induced by 5 µ M benzyladenine (BA). Spermine and putrescine were more efficient as BA antagonists than cadaverine and spermidine. Polyamines affected posttranscriptional stages of BA action but did not interfere with the accumulation of cytokinin-dependent mRNA. This fact contradicts the hypotheses about the existence of a common receptor for polyamines and BA or about polyamines as second messengers in the cytokinin signaling pathway. In Arabidopsis seedlings, exogenous polyamines suppressed BA effects at low (micromolar) concentrations comparable with BA concentrations. The incubation of Arabidopsis seedlings with aminoguanidine (an inhibitor of putrescine and cadaverine catabolism) for 24 h resulted in a significant reduction in the activity of the cytokinin primary response gene. These data indicate that endogenous polyamines can modulate considerably the amplitude of the cytokinin primary response gene expression in vivo.Abbreviations and designations : AG-aminoguanidine; BAbenzyladenine; MU-4-methylunbelliferone; MUG-4-methylumbelliferyl-β -D-glucuronide; P ARR5 : GUS -the reporter GUS gene under the control of the promoter of the cytokinin-dependent ARR5 gene.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polyamines suppress manifestation of cytokinin primary effects

Rakova

Romanov

2005

Russ J Plant Physiol

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“…Phospholipase D (PLD, EC 3.1.4.4.) was the first to be suggested as a component of the molecular machinery for the cytokinin signal transduction in Amaranthus (Romanov et al 2000) and Arabidopsis (Romanov et al 2002, 2003) plants. Then phospholipase C has been suggested to be involved in cytokinin response in the moss Physcomitrella patens (Repp et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Initial evidence for PLD participation in the cytokinin response was based on a dose–dependent inhibition of cytokinin action by low concentrations of primary alcohols (Romanov et al 2000, 2002, 2003), potent specific inhibitors of phosphatidic acid formation by PLD (Ella et al 1997, Yu et al 1996). Secondary alcohols, very close in properties and structure to corresponding primary alcohols but incapable to interfere with PLD action, lack such inhibitory effect.…”

Section: Introductionmentioning

confidence: 99%

Rapid activation of specific phospholipase(s) D by cytokinin inAmaranthusassay system

Kravets

Kolesnikov

Kretynin

et al. 2010

Physiologia Plantarum

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The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using the level of phosphatidylbutanol production as a measure of PLD activity. Rapid activation of phosphatidylbutanol synthesis was demonstrated as early as within 5 min of cytokinin administration. Neomycin, a known phosphatidylinositol-4,5-bisphosphate (PIP(2)) antagonist, strongly repressed both physiological cytokinin effect and cytokinin-dependent PLD activation. N-acylethanolamine (NAE 12), an inhibitor of alpha-class PLD, did not influence significantly cytokinin effect on Amaranthus seedlings. Together, results suggest the involvement of PIP(2)-dependent non-class alpha-PLD in the molecular mechanism of cytokinin action.

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How do cytokinins affect the cell?

Romanov

2009

Russ J Plant Physiol

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Primary Alcohols, Substrates for Phospholipase D-Catalyzed Transphosphatidylation, Suppress the Cytokinin Action

Cited by 4 publications

References 14 publications

Polyamines suppress manifestation of cytokinin primary effects

Polyamines suppress manifestation of cytokinin primary effects

Rapid activation of specific phospholipase(s) D by cytokinin inAmaranthusassay system

How do cytokinins affect the cell?

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