Primary and secondary inner filtering. Effect of K2Cr2O7 on fluorescence emission intensities of quinine sulfate

Tucker, Sheryl A.; Amszi, Vicki L.; Acree, William E.

doi:10.1021/ed069pa8

Cited by 102 publications

(63 citation statements)

References 4 publications

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“…Samples were run with slit widths set at 5 nm for both Ex and Em modes, an integration time of 0.0125 s, scan rate of 2,400 nm m −1 , and at either 800 or 950 V, depending on sample response. Fluorescence measurements were corrected for excitation energy and emission detector response using correction factors supplied by the manufacturer and any innerfilter effects were corrected following Tucker et al (1992). Final Ex, peak excitation wavelength(s); Em, peak emission wavelength(s).…”

Section: Optical Measurementsmentioning

confidence: 99%

Formation of Chromophoric Dissolved Organic Matter by Bacterial Degradation of Phytoplankton-Derived Aggregates

et al. 2018

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Organic matter produced and released by phytoplankton during growth is processed by heterotrophic bacterial communities that transform dissolved organic matter into biomass and recycle inorganic nutrients, fueling microbial food web interactions. Bacterial transformation of phytoplankton-derived organic matter also plays a poorly known role in the formation of chromophoric dissolved organic matter (CDOM) which is ubiquitous in the ocean. Despite the importance of organic matter cycling, growth of phytoplankton and activities of heterotrophic bacterial communities are rarely measured in concert. To investigate CDOM formation mediated by microbial processing of phytoplankton-derived aggregates, we conducted growth experiments with non-axenic monocultures of three diatoms (Skeletonema grethae, Leptocylindrus hargravesii, Coscinodiscus sp.) and one haptophyte (Phaeocystis globosa). Phytoplankton biomass, carbon concentrations, CDOM and base-extracted particulate organic matter (BEPOM) fluorescence, along with bacterial abundance and hydrolytic enzyme activities (α-glucosidase, β-glucosidase, leucine-aminopeptidase) were measured during exponential growth and stationary phase (∼3-6 weeks) and following 6 weeks of degradation. Incubations were performed in rotating glass bottles to keep cells suspended, promoting cell coagulation and, thus, formation of macroscopic aggregates (marine snow), more similar to surface ocean processes. Maximum carbon concentrations, enzyme activities, and BEPOM fluorescence occurred during stationary phase. Net DOC concentrations (0.19-0.46 mg C L −1 ) increased on the same order as open ocean concentrations. CDOM fluorescence was dominated by protein-like signals that increased throughout growth and degradation becoming increasingly humic-like, implying the production of more complex molecules from planktonic-precursors mediated by microbial processing. Our experimental results suggest that at least a portion of open-ocean CDOM is produced by autochthonous processes and aggregation likely facilitates microbial reprocessing of organic matter into refractory DOM.

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Section: Optical Measurementsmentioning

confidence: 99%

Formation of Chromophoric Dissolved Organic Matter by Bacterial Degradation of Phytoplankton-Derived Aggregates

et al. 2018

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“…The SIFE is not negligible because optical density of the absorber (-carotene) in emission wavelengths is not low. If samples absorb at both wavelengths: the fluorescence excitation and emission, the correction of the observed fluorescence for (IFE) is necessary (Tucker et al, 1992). This is the case of -carotene in RTILs, the fluorescence excitation wavelength is at 413 nm and maximum of fluorescence emission at around 530 nm in standard cell.…”

Section: Resultsmentioning

confidence: 99%

Inner Filter Effect in the Fluorescence Emission Spectra of Room Temperature Ionic Liquids with-β-Carotene

Pawlak¹,

Skrzypczak²,

Bialek-Bylka³

2011

Applications of Ionic Liquids in Science and Technology

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“…For high absorbing samples fluorescence signals were corrected for self-absorbance where appropriate, using primary and secondary inner-filtering correction methodologies. [40][41][42] All the measurements were taken in triplicate and averaged. Finally, data analysis was performed using Microsoft Excel and SigmaPlot softwares.…”

Section: Methodsmentioning

confidence: 99%

Self‐Probing of Micellization within Phenyl‐Containing Surfactant Solutions

Baker

Behera

et al. 2010

ChemPhysChem

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Primary and secondary inner filtering. Effect of K2Cr2O7 on fluorescence emission intensities of quinine sulfate

Abstract: Fluorescence spectroscopy is an extremely versatile, sensitive experimental technique used in identification and quantification of many environmentally important compounds.

Cited by 102 publications

References 4 publications

Formation of Chromophoric Dissolved Organic Matter by Bacterial Degradation of Phytoplankton-Derived Aggregates

Formation of Chromophoric Dissolved Organic Matter by Bacterial Degradation of Phytoplankton-Derived Aggregates

Inner Filter Effect in the Fluorescence Emission Spectra of Room Temperature Ionic Liquids with-β-Carotene

Self‐Probing of Micellization within Phenyl‐Containing Surfactant Solutions

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