Primary Bioassay of Human Myeloma Stem Cells

Hamburger, Anne W.; Salmon, Sydney E.

doi:10.1172/jci108839

Cited by 406 publications

(136 citation statements)

References 27 publications

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“…Only a short (7 days) incubation period is needed, thus avoiding both the delay and increased chance of contamination inherent in longer times in culture (Hamburger & Salmon, 1977 (Hamburger & Salmon, 1977) lymphoma, 0.001-1 1% (Jones et al, 1979) and normal B cells, less than 0-014% (Radnay et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Colony formation by normal and malignant human B-lymphocytes

Izaguirre¹,

Minden²,

Howatson³

et al. 1980

Br J Cancer

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Re(ei v\e(d I I AIarch 1(90 A\(cepted 16 May 1 981) Summary.-A method is described that permits colony formation in culture by B lymphocytes from normal blood and from blood, marrow or lymph nodes of patients with myeloma or lymphoma. The method depends on: (1) exhaustively depleting cell suspensions of T lymphocytes, (2) a medium conditioned by T lymphocytes in the presence of phytohaemagglutinin (PHA-TCM), and (3) irradiated autologous or homologous T lymphocytes. Under these conditions the assay is linear. Cellular development of B lymphocytes can be followed; differentiation to plasma cells is seen in cultures of cells from normal individuals and myeloma patients, but not lymphoma patients. Malignant B lymphocytes in culture produced immunoglobulin of the class identified in the patient's blood, or in freshly obtained cells. We conclude that the assay is suitable for studying the growth, differentiation and regulation of normal and malignant B lymphocytes in culture.

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Section: Discussionmentioning

confidence: 99%

Colony formation by normal and malignant human B-lymphocytes

Izaguirre¹,

Minden²,

Howatson³

et al. 1980

Br J Cancer

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“…In 1963, over four decades ago, Bruce and Van Der Gaag were the first to suggest the existence of cancer initiating cells (CICs) in murine lymphoma and a method for their in vivo quantification [17]. In 1977, Hamburger et al published a method for supporting colony growth of human tumor stem cells in soft agar [18,19]. Buick et al developed an in vitro system for measuring the frequency of clonogenic cells within tumors more accurately in semi-solid cultures [20,21].…”

Section: Cancer Stem Cells-past and Currentmentioning

confidence: 99%

Targeted therapy aimed at cancer stem cells: Wilms’ tumor as an example

2013

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Wilms' tumor (WT), a common renal pediatric solid tumor, serves as a model for a malignancy formed by renal precursor cells that have failed to differentiate properly. Here we review recent evidence showing that the tumors' heterogeneous cell population contains a small fraction of cancer stem cells (CSC) identified by two markers: Neural Cell Adhesion Molecule 1 (NCAM1) expression and Aldehyde dehydrogenase 1 (ALDH1) enzymatic activity. In vivo studies show these CSCs to both self-renew and differentiate to give rise to all tumor components. Similar to other malignancies, the identification of a specific CSC fraction has allowed the examination of a novel targeted therapy, aimed at eradicating the CSC population. The loss of CSCs abolishes the tumor's ability to sustain and propagate, hence, causing tumor degradation with minimal damage to normal tissue.

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“…MM cell colonies that consist of 440 cells were counted under an inverted microscope. 5 Hematopoietic stem cell colonies were classified and counted as granulocyte-macrophage colony-forming units or erythroid burst-forming units.…”

Section: Colony-forming Assaysmentioning

confidence: 99%

“…Hamburger and Salmon 5 have reported that 486% of tumor samples from MM patients were capable of colony formation at a frequency of 1 in 1000 to 100 000 cells. Because of their extreme rarity, alternative approaches have also been developed.…”

Section: Introductionmentioning

confidence: 99%