Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro

Alsemgeest, S.P.M.; Klooster, G.A.E. van ’t; Miert, A. S. J. P. A. M. van; Hulskamp-Koch, C. K.; Gruys, E.

doi:10.1016/0165-2427(96)05602-4

Cited by 53 publications

(36 citation statements)

References 10 publications

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“…Young et al (1995) found that the elevated Hp concentrations, determined in the last phase of pregnancy and after delivery may be connected with the changing hormone profile (the influence of estrogens and progesterone). It is worth pointing out that the increase in Hp concentration in cattle is also caused by the increased concentration of cortisol (Alsemgeest et al 1996). The fact of the increased SAA concentrations can be interpreted in a similar way.…”

Section: Kinetic Variables Ofmentioning

confidence: 85%

Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

et al. 2009

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The objective of this study was to evaluate the concentrations of selected acute phase proteins -haptoglobin (Hp) and serum amyloid A (SAA), and selected variables of energy metabolism in blood serum of dairy cows during different stages of the pre-and postpartal period. The analyses were performed in dairy cows of the Slovak spotted breed and its crossbreeds (n = 57). The cows were divided into 9 groups according to the evaluated part of reproduction cycle -from 4 weeks before parturition to 10 weeks after parturition. Significant differences were found in mean Hp and SAA concentrations during the monitored period (P < 0.05 and P < 0.001, respectively). Cows during the early postparturient period had significantly higher Hp and SAA concentrations compared to cows in later postparturient period (P < 0.05). Significant differences throughout the period under study was found also in means of total cholesterol (P < 0.01), triglycerides (P < 0.001), and non-esterified fatty acids (P < 0.001). Concentrations of Hp during this period were positively correlated with SAA (R = 0.919; P < 0.001). Moreover, significant correlations were found between Hp and non-esterified fatty acids, Hp and β-hydroxybutyrate, as well as between SAA and non-esterified fatty acids. Our results indicate that the acute phase response occurs in cows around parturition, and suggest that there are relationships between mediators of immune response and several indices of energy metabolism. Our results also suggest that in cows with higher concentrations of non-esterified fatty acids, indicating a certain degree of lipid mobilisation, higher values of acute phase proteins may be found. Haptoglobin, serum amyloid A, energy balance, cows, periparturient period

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Section: Kinetic Variables Ofmentioning

confidence: 85%

Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

et al. 2009

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“…IL-6 increases in the serum after LPS stimulation or E. coli mastitis [19,27], and it has been shown to be a potent inducer of hepatic production of SAA [2]. In milk, concentrations of TNFα and SAA seemed to be closely intertwined; cows with very low TNFα also had low SAA concentrations, and those with high TNFα had high SAA concentrations.…”

Section: Discussionmentioning

confidence: 99%

Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

2004

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-A cross-over study was conducted to investigate the effect of intramammarily infused lipopolysaccharide (LPS) on the acute phase reaction in early (EL) and in late (LL) lactation. Nine cows received intramammary injections of 100 µg of Escherichia coli 0111:B4 LPS during EL and LL. The severity of each cows systemic and local signs and change in milk appearance were recorded and scored throughout the experiment. Systemic and local signs were found to be more serious in EL cows. Tumor necrosis factor α (TNFα) was detected in milk but not in serum. Serum amyloid A (SAA) concentrations increased both in serum and in milk. The milk TNFα concentrations peaked at 8 h post-challenge (PC). SAA concentrations started to increase at 8 h PC, and peak concentrations were seen at 32 and 48 h PC in milk and serum, respectively. The milk TNFα and SAA seemed to be correlated, being on average higher in EL. Serum SAA concentration was not correlated with milk TNFα or SAA, nor with the severity of local or systemic signs, but was correlated with changes in milk appearance. LPS / mastitis / dairy cow / SAA / TNFα

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“…APPs estimation may be used to help monitor the health and welfare of production animals on farms (Eckersall 2000). According to Alsemgeest et al (1996), increased APP concentrations in cattle could be due to an increased concentration of cortisol as part of the response to stress. The albumin value, which is known to be one of the negative APPs, since its concentration decreases in response to challenge (Mackiewicz 1997;Paulina & Tadeusz 2011), was significantly lower during the restructuring (T2) and the reference range value suggested by Kaneko et al (1997) (3.03-3.55 g/dL) and Cozzi et al (2011) (37 g/L).…”

Section: Discussionmentioning

confidence: 99%

Effects of abrupt housing changes on the welfare of Piedmontese cows

Tarantola

Valle

Marco

et al. 2016

Italian Journal of Animal Science

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The Italian Piedmontese cattle breed is traditionally housed in tie-stalls and, to a lesser extent, in loose housing systems with free stalls. The present study has compared the same group of 15 cattle before and after stable renovation (tie-stall vs free-stall housing) funded by Regulation (EU) No. 1305/2013. All the animals remained healthy during the trial, no clinical signs were observed and no mastitis occurred. The tested parameters were: locomotion scoring system, salivary cortisol, blood parameters, serum acute phase protein (albumin, haptoglobin, serum amyloid A and lysozyme). Samples were collected 3 times: before the change (T1), 3 days later (T2), 40 days later (T3). The change in housing determined noteworthy variations in the stress parameters: albumin and total protein displayed the lowest value at T2, while lysozyme displayed the lowest value at T3. Among the App, SAA and Hp were not affected by the sampling time. Salivary cortisol displayed the highest value at T1. This study suggests that tie-stall housing can endanger the welfare of animals, and it is hoped that this farming system will be abandoned in the future. ARTICLE HISTORY

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Primary bovine hepatocytes in the study of cytokine induced acute-phase protein secretion in vitro

Cited by 53 publications

References 10 publications

Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

Acute Phase Proteins and their Relation to Energy Metabolites in Dairy Cows during the Pre- and Postpartal Period

Serum amyloid A and TNF? in serum and milk during experimental endotoxin mastitis

Effects of abrupt housing changes on the welfare of Piedmontese cows

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