Primary chicken embryo fibroblasts seeded acellular dermal matrix (3-D ADM) improve regeneration of full thickness skin wounds in rats

Gangwar, Anil Kumar; Kumar, Naveen; Khangembam, Sangeeta Devi; Kumar, Vineet; Singh, Rajendra

doi:10.1016/j.tice.2015.04.002

Cited by 26 publications

(10 citation statements)

References 54 publications

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“…Neutrophilia for short duration suggested the effect of surgical trauma rather than the implant provoked response. Similar observations were also reported by Gangwar et al [ 31 ] following application of acellular dermal matrix for full thickness skin graft in rats.…”

Section: Discussionsupporting

confidence: 90%

Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

et al. 2016

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An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

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Section: Discussionsupporting

confidence: 90%

Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

et al. 2016

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“…In all the buffaloes, CADM was implanted between the parietal peritoneum and internal sheath of the rectus abdominis muscle without serious complications. The advantages of the use of allogenic or xenogenic acellular matrices for the repair of soft tissue defects are well established in many experimental models . These acellular matrices are slowly degraded by cellular proteases at the implantation site and are replaced by new endogenous extracellular matrix proteins secreted by ingrowing fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…Further, these matrices stimulate rapid neovascularization during tissue regeneration and are relatively inert immunologically . They are resistant to infections, a feature that may be attributable to their inherent anti‐microbial activity and/or their ability to rapidly vascularize and therefore clear bacteria. Hematological and biochemical findings of the present study further supported the use of xenogenic acellular matrix for the repair of soft tissue defects.…”

Section: Discussionmentioning

confidence: 99%

Preparation, characterization, and xenotransplantation of the caprine acellular dermal matrix

Asodiya

Kumar

Vora

et al. 2019

Xenotransplantation

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Background Caprine skin is a promising biomaterial for tissue‐engineering applications. However, tissue processing is required before its xenogenic use. Aims Therefore, the purpose of this study was to evaluate the structural integrity and biocompatibility of the caprine skin after de‐epithelialization, using sodium chloride (NaCl) and trypsin solutions, followed by de‐cellularization using sodium dodecyl sulfate (SDS) solution. Materials & Methods The caprine skin was de‐epithelialized using NaCl (2‐4 mol/L) and trypsin (0.25%‐0.5%) followed by the treatment of SDS (1%‐4%) solution over a period of time. Acellularity of the prepared matrix was confirmed histologically and characterized by appropriate staining, scanning electron microscopy (SEM), DNA quantification, and Fourier‐transform infrared (FTIR) spectroscopy. The caprine acellular dermal matrix (CADM) was used for the repair of spontaneously occurring abdominal hernia in ten buffaloes. The biocompatibility of the CADM was evaluated using clinical, hematological, biochemical, and anti‐oxidant parameters. Results Histologically, the skin treated with 0.25% trypsin in 4 mol/L NaCl for 8 hours resulted in complete de‐epithelialization. Further treatment with 2% SDS for 48 hours demonstrated complete acellularity and orderly arranged collagen fibers. The SEM confirmed a preservation of collagen arrangement within CADM. The DNA content was significantly (P < .05) lower in CADM (46.20 ± 7.94 ng/mg) as compared to fresh skin (662.56 ± 156.11 ng/mg) indicating effective acellularity. The FTIR spectra showed characteristic collagen peaks of amide A, amide B, amide I, amide II, and amide III in CADM. All the 10 animals recovered uneventfully and remained sound. Hematological, biochemical, and anti‐oxidants findings were unremarkable. Conclusion Results indicated the acceptance and biocompatibility of the xenogenic caprine acellular dermal matrix for abdominal hernia repair in buffaloes without complications.

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“…Figure 5 depicts liver and muscle SOD activities did not show remarkable statistical deference, but enzyme activities of serum in PHBV group were significantly higher than the control group ones valued (p < 0.001). In one study, Gangwar et al [26] created full thickness skin wounds in rats. The wounds for group 1 were dressed, defects for group 2 were repaired with acellular dermal matrix (ADM).…”

Section: Resultsmentioning

confidence: 99%

Effects of bacterial PHBV-conduit used for nerve regeneration on oxidative stress parameters in rats

Erol-Demirbilek

Demirbilek

Erdal

et al. 2020

Hacettepe Journal of Biology and Chemistry

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N öronal hücrelerin kendi kendilerini tamir mekanizmaları olmamasından dolayı, hasarlı sinir dokularının rejenerasyonunda biyomateryaller yaygın bir şekilde çalışılmıştır. Avantajları olmasına rağmen, nanomateryaller oksidatif strese neden olabilir ve bu durum tedaviyi etkileyebilir. Bu çalışmada, aksonal rejenerasyon için kullanılan PHBV [poly (3-hydroxybutyrateco-3-hydroxyvalerate)]'nin ratlarda lipid peroksidasyonuna ve protein oksidayonuna neden olup olmadığı ve ayrıca antioksidan molekülleri etkileyip etkilemediği araştırıldı. Çalışmada, elektrospinning ile PHBV yönlendirilmiş nanofiber membranlar hazırlandı ve bunlar kullanılarak graftler oluşturuldu. Sıçan peritoneal sinirlerinde 1 cm boşluk oluşturulduktan sonra PHBV graftler yerleştirildi. Hayvanlar operasyon sonrası 17. haftada feda edildi. Ratların kan, karaciğer ve graft yerleştirilen perioneal sinir demeti çevre kas dokusu malondialdehit (MDA), ileri oksidasyon protein ürünleri (AOPP), glutatyon (GSH) seviyeleri ve süperoksit dismutaz (SOD) aktiviteleri ölçüldü. Kontrol grubu ile karşılaştırıldığında, tüm graft grubu serum MDA, AOPP ve GSH düzeyleri ve SOD aktivitelerinde anlamlı bir artış gözlenirken, dokularda sadece MDA ve AOPP seviyeleri istatistiksel olarak yüksek bulundu. Bu bulgular perioneal sinir defektleri için kullanılan PHBV graftinin sıçanlarda oksidatif strese neden olabileceğini düşündürmektedir.

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Primary chicken embryo fibroblasts seeded acellular dermal matrix (3-D ADM) improve regeneration of full thickness skin wounds in rats

Cited by 26 publications

References 54 publications

Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Preparation, characterization, and xenotransplantation of the caprine acellular dermal matrix

Effects of bacterial PHBV-conduit used for nerve regeneration on oxidative stress parameters in rats

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