Primary culture of adult mouse lung fibroblasts in serum-free medium: Responses to growth factors

Interferon Regulatory Factor-1 Regulates the Autophagic Response in LPS-Stimulated Macrophages through Nitric Oxide

“…Primary lung fibroblasts isolated from MIF knockout (mif -/-) (17) or wild type C57BL6 mice (mif +/+ ) were established by outgrowth from lung explants (18) and used during passages 2-6.…”

Methanol extract of Dioscoreae Rhizoma inhibits pro-inflammatory cytokines and mediators in the synoviocytes of rheumatoid arthritis

“…Mouse lung fibroblast cells (mLFCs) were isolated from BALB/mice as described by Kumar et al [33], with some minor modifications. In brief, lungs were dissected from BALB/mice and placed in a petri dish containing 10 ml of Hanks' balanced salt solution with 100 U/ml of penicillin and 100 Ag/ml of streptomycin.…”

Epidermal growth factor-like molecular species in normal bronchoalveolar lavage fluid

“…The presence of EGF receptor-binding molecular species in five-fold concentrated BALF samples was assessed by their ability to inhibit the binding of receptor-grade mouse EGF (Sigma), labeled with 125I by the Iodogen method to a specific activity of 25 50 mCi/mg, to adult BALB/c mouse lung fibroblasts (MLF). These cells were established in serum-free primary culture on collagen-coated surfaces in medium MCDB 201 supplemented with fatty acid-free BSA (5 g/liter), lipids from bovine serum (1.5 ml/liter), EGF (15 ixg/liter) (all from Sigma), partially saturated human transferrin (Boehringer Mannheim, Sydney) (30 mg/liter), and other minor constituents as described [11]. MLF at passage 2 were seeded at ~40,000 cells/cm 2 in collagen-coated 24-well culture plates (Nunc, Roskilde, Denmark) and rendered noncycling by incubation in EGF-deficient medium for 96 h. The use of a sequential assay ensured that any growth factor-binding protein(s) that might have been present in BALF did not lead to spurious detection [10].…”