Primary culture of mantle cells of bivalve mollusc, Paphia malabarica

Dessai, Shanti N.

doi:10.1007/s11626-012-9538-4

Cited by 17 publications

(15 citation statements)

References 14 publications

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“…The cells started migrating and emanating from the explants within 24 h post-incubation and formed aggregates. The liberated cells were mostly round and found to be either granulocytes or hyalinocytes, with granulocytes dominating the culture as reported by Li et al (1966), Suja and Dharmaraj (2005) and Dessai (2012). Cell liberation was seen from the periphery of the explant.…”

supporting

confidence: 67%

“…Cell density was found to be good when the plates were incubated at 25°C. Suja et al (2005) and Dessai (2012) found that the cell density was higher at 28°C and 20-25°C, respectively. This suggests that incubation temperature could be one of the determining factors in the growth and proliferation of mantle epithelial cells.…”

mentioning

confidence: 97%

“…The primary culture of outer epithelial cells of the mantle tissue gives an insight to the pearl formation process. The primary cultures of mantle cells from various molluscs such as abalone (Suja and Dharmaraj, 2005;Suja et al, 2007), clam (Koyama and Aizawa, 2000;Dessai, 2012), mussel (Cornet, 2006;Quinn et al, 2009), scallops (Endoh and Hasegawa, 2006) and oysters (Awaji, 1997;Gong et al, 2008) have been obtained.…”

mentioning

confidence: 99%

“…The fibroblast-like cells were found to have different sizes ranging from small to large spindle shaped cells. Suja et al (2005) reported the appearance of fibroblast-like cells on day 2 post-incubation in in vitro mantle tissue culture of abalone Haliotis varia and Dessai (2012) reported the appearance of spindle shaped cells on day 2 in Paphia malabarica. Daugavet and Blinova (2015) also reported the presence of fibroblast-like cells in the early stages of mantle cell culture in the blue mussel, Mytilus edulis.…”

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confidence: 99%

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Evaluation of different media for cell proliferation in mantle tissue culture of the green mussel, Perna viridis (Linnaeus, 1758)

et al. 2017

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The aim of the present study was to establish a suitable culture system for tissue explants from the mantle of the green mussel, Perna viridis. The experiments were conducted using healthy, live green mussels in the size range of 75 to 110 g collected from Pulicat Lake, Tamil Nadu. Three different culture media namely M199, Leibovitz L-15 and sterile seawater were used to assess the most suitable medium for growth, proliferation and viability of mantle epithelial cells. The effect of the addition of two supplements viz., 10% foetal calf serum (FCS) and 0.1% yeast extract to the culture media was also evaluated. After carefully isolating the pallial layer from the mantle tissue, 1-2 mm2 size explants were successfully cultured in 12-well plates at 25°C for up to 14 days. Cultures were monitored under light and phase contrast objectives in an inverted microscope. Cell counts were made and cell size was measured for each treatment. Cells were observed to migrate from the periphery of the explant within 24 h after initiation of cultures. The liberated cells were mostly round and were either granulocytes or hyalinocytes. Fibroblast-like cells were also observed. Our results showed that proliferation of epithelial cells from mantle tissue was maximum in seawater medium (7.4x104 cells ml-1), followed by L-15 medium (2.55x104 cells ml-1). Average cell size in seawater medium was 10.72 μm and that in L-15 and M199 media was 8.56 and 6.39 μm, respectively. Adherent cells were also more prominent and higher in number in seawater medium. Supplementation of culture media with 10% FCS and 0.1% yeast extract improved both cell proliferation and cell size in all the three culture media. Four concentrations of 0.1% yeast extract (@ 50 μl, 75 μl, 100 μl, 150 μl ml-1 medium) were tested in the present study and best results were obtained with 100 μl ml-1, with respect to both cell counts and size.

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confidence: 97%

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confidence: 99%

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Evaluation of different media for cell proliferation in mantle tissue culture of the green mussel, Perna viridis (Linnaeus, 1758)

et al. 2017

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“…9,10 Recently, an in vitro approach based on haemocytes of the clam Ruditapes philippinarum was used to investigate the effects of TiO 2 nanoparticles on the phagocytic activity 10 while efforts to establish primary cell cultures from the mantle of the clam P. malabarica were also performed. 11 Regarding the toxicity exerted by nanoparticles in a variety of taxa, details were also described 12 of how nanoparticles may interact with organisms through different ways such as adsorption to the surface (cell, organ or body), cellular internalization, or dissolution of ions † Electronic supplementary information (ESI) available: Micrographs of semithin sections of the gills as well as the typical identification of electron-dense contrasts by EDX analysis. See DOI: 10.1039/c4an01643b a Instituto de Ciencia de Materiales de Sevilla (CSIC -Univ.…”

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confidence: 99%

STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

García-Negrete

Haro

Blasco

et al. 2015

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The methodology termed scanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) has been used in this work to study the uptake of citrate stabilized gold nanoparticles (AuNPs) (average particle sizes of 23.5 ± 4.0 nm) into tissue samples upon in vitro exposure of the dissected gills of the Ruditapes philippinarum marine bivalve to the nanoparticle suspensions. The STEM-in-SEM methodology has been optimized for achieving optimum resolution under SEM low voltage operating conditions (20-30 kV). Based on scanning microscope assessments and resolution testing (SMART), resolutions well below 10 nm were appropriately achieved by working at magnifications over 100k×, with experimental sample thickness between 300 and 200 nm. These relatively thick slices appear to be stable under the beam and help avoid NP displacement during cutting. We herein show that both localizing of the internalized nanoparticles and imaging of ultrastructural disturbances in gill tissues are strongly accessible due to the improved resolution, even at sample thicknesses higher than those normally employed in standard TEM techniques at higher voltages. Ultrastructural imaging of bio-nano features in bioaccumulation experiments have been demonstrated in this study.

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Lipoprotein-induced cell growth and hemocyanin biosynthesis in rhogocytes

et al. 2022

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Rhogocyte is a unique molluscan cell that synthesises a supramolecular respiratory protein known as hemocyanin. Its ability to synthesise the protein has eluded the scientists despite hemocyanin’s importance as a carrier protein and complex molecule with anti-viral activity. Although a hypothetical model of hemocyanin release from the rhogocytes lacunae was proposed based on colloid-osmotic pressure mechanism, lack of in vitro studies limits further validation of this model. In this study, we aim to investigate the impact of cell culture conditions and nature of hemocyanin biosynthesis of rhogocyte cells dissociated from Haliotis laevigata mantle tissue. Population of cells with different hemocyanin expression levels was profiled using flow cytometry, while hemocyanin concentrations in the media were elucidated by ELISA assay. We demonstrated that addition of lipoprotein supplement into the media resulted in a burst secretion of hemocyanin into the culture media. Over 7 days of culture, the population of cells tagged with hemocyanin antibody increased steadily while hemocyanin release in the media decreased significantly. Variation of culture medium, temperature, growth supplement type and concentration also impacted the cell growth and hemocyanin biosynthesis. These results indicated the possibility of an active process triggered by the addition of supplement to synthesise the protein at the highest amount during the first hour. The current study provides a glimpse of the hemocyanin biosynthesis by rhogocyte that may be significant to understand the cell ability to synthesise supramolecular protein and secretion through lacunae.

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Primary culture of mantle cells of bivalve mollusc, Paphia malabarica

Cited by 17 publications

References 14 publications

Evaluation of different media for cell proliferation in mantle tissue culture of the green mussel, Perna viridis (Linnaeus, 1758)

Evaluation of different media for cell proliferation in mantle tissue culture of the green mussel, Perna viridis (Linnaeus, 1758)

STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

Lipoprotein-induced cell growth and hemocyanin biosynthesis in rhogocytes

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