Primary Screening of the Bioactivity of Brackishwater Cyanobacteria: Toxicity of Crude Extracts to Artemia salina Larvae and Paracentrotus lividus Embryos

Lopes, Viviana R.; Fernández, Narciso de Gabriel; Martíns, Rosário; Vasconcelos, Vı́tor

doi:10.3390/md8030471

Cited by 43 publications

(27 citation statements)

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“…Neurotoxic and hepatotoxic effects as well antialgal, immunosuppressive, antimycotic, antibacterial, antitumour and antiviral activity have been described ( Gademann and Portmann, 2008, Hirata et al, 2003, Jaiswal et al, 2008, Martins et al, 2007, Martins et al, 2008, Mundt et al, 2001and Wiegand and Pflugmacher, 2005. On the other hand, recent studies have already demonstrated that cyanobacteria isolated from estuaries induce toxic effects on marine invertebrates ( Lopes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

Lopes

Schmidtke

Fernandes

et al. 2011

Toxicology in Vitro

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The cyanobacteria are known to be a rich source of metabolites with a variety of biological activities in different biological systems. In the present work, the bioactivity of aqueous and organic (methanolic and hexane) crude extracts of cyanobacteria isolated from estuarine ecosystems was studied using different bioassays. The assessment of DNA damage on the SOS gene repair region of mutant PQ37 strain of Escherichia coli was performed. Antiviral activity was evaluated against influenza virus, HRV-2, CVB3and HSV-1 viruses using crystal violet dye uptake on HeLa, MDCK and GMK cell lines. Cytotoxicity evaluation was performed with L929 fibroblasts by MTT assay. Of a total of 18 cyanobacterial isolates studied, only the crude methanolic extract of LEGE 06078 proved to be genotoxic (IF > 1.5) in a dosedependent manner and other four were putative candidates to induce DNA damage. Furthermore, the crude aqueous extract of LEGE 07085 showed anti-herpes type 1 activity (IC50 = 174.10 μg dry extract mL −1 )while not presenting any cytotoxic activity against GMK cell lines. Of the 54 cyanobacterial extracts tested, only the crude methanolic and hexane ones showed impair on metabolic activity of L929 fibroblasts after long exposure (48-72 h). The inhibition of HSV-1 and the strong cytotoxicity against L929 cells observed emphasizes the importance of evaluating the impact of those estuarine cyanobacteria on aquatic ecosystem and on human health. The data also point out their potential application in HSV-1 treatment and pharmacological interest.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

Lopes

Schmidtke

Fernandes

et al. 2011

Toxicology in Vitro

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“…Furthermore, the presence of anabaenopeptins A and D in a benthic brackish-water Nostoc isolate from an Atlantic estuary (Minho River, Portugal) has been reported by Lopes and co-workers (unpublished information). Moreover, Lopes et al (2010) and have shown that benthic cyanobacteria from Atlantic estuaries (e.g. Synechococcus,…”

Section: Further Cyanotoxinsmentioning

confidence: 99%

“…E-mail: vmvascon@ fc.up.pt Tan, 2007). The cyanobacteria of brackish waters (including estuaries), however, remain under-investigated, particularly in Europe, despite a number of significant contributions (Sivonen et al, 1989;Nehring, 1993;Lehtima¨ki et al, 1994Lehtima¨ki et al, , 1997Lehtima¨ki et al, , 2000Wille´n & Mattson, 1997;Sivonen & Jones, 1999;Sipia¨et al, 2001Sipia¨et al, , 2002Sipia¨et al, , 2006Sipia¨et al, , 2007Sipia¨et al, , 2008Vasconcelos & Cerqueira, 2001;Laamanen et al, 2002;Rocha et al, 2002;Geiss et al, 2003;Hobson & Fallowfield, 2003;Lehtonen et al, 2003;Stal et al, 2003;Sobrino et al, 2004;Herfindal et al, 2005;Kankaanpa¨a¨et al, 2005;Karjalainen et al, 2005Karjalainen et al, , 2007Karlsson et al, 2005;Pere´z & Carrilo, 2005;Surakka et al, 2005;Marino et al, 2006;Mazur-Marzec et al, 2007Paoli et al, 2007;Halinen et al, 2008;Ibelings & Havens, 2008;Jo´z´wiak et al, 2008;Lopes et al, 2010;Oftedal et al, 2010;). This lack of information for brackish-water cyanobacteria is especially significant in relation to the potential for production of new bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Lopes

Vasconcelos

2011

European Journal of Phycology

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Cyanobacteria are photosynthetic organisms found in aquatic and terrestrial biotopes and ecosystems throughout the world. Marine and freshwater cyanobacteria have been extensively studied due to the toxic hazards that some of them create and biotechnological interest. In contrast, the cyanobacteria of brackish waters have been less studied despite being able to form toxic blooms like their freshwater and marine counterparts. We review the occurrence, diversity and toxicity of cyanobacteria species in the brackish waters of Europe, mainly estuaries and brackish environments of the Atlantic Ocean and the Mediterranean and Baltic Seas. The dominant cyanobacteria belong mainly to the planktonic genera Nodularia, Aphanizomenon, Microcystis and Anabaena, but also the benthic forms of Anabaena and Phormidium. Most genera from brackish waters are reported to be hepatotoxin producers (microcystin and nodularin). However, anatoxin-a and other bioactive compounds (e.g. apoptogens) can also be found and are produced exclusively by benthic forms. Nodularin is the best-characterized brackish-water cyanotoxin, being primarily produced by N. spumigena. Data are presented on cyanotoxin production, accumulation, and potential food chain transfer, with a particular focus on nodularin, which induces multiple effects on food-chain dynamics. Microcystins and anatoxin-a are also considered. Potential risks to animals and humans from cyanotoxin exposure are discussed, although reports of animal poisoning by brackish-water toxic cyanobacteria are scarce and no cases of human intoxication are yet known.

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“…Nonaxenic and uni-cyanobacterial cultures were posteriorly lyophilized. The taxonomic characterization was performed as described before by Lopes et al (2010).…”

Section: Cyanobacteria Isolation and Culturementioning

confidence: 99%

“…Trichloroacetic acid (TCA), Methanol/Acetone and hydrochloric acid (HCl) have been evaluated to extract BMAA from cyanobacterial samples (Banack et al 2007;Eriksson et al 2009). Samples were collected and made unialgal by the standard methods (Lopes et al 2010). Eighteen different strains were analyzed for the presence of BMAA, in what, as far as we know, is the first report of BMAA presence in estuaries.…”

Section: Introductionmentioning

confidence: 99%

The non-protein amino acid β-N-methylamino-l-alanine in Portuguese cyanobacterial isolates

et al. 2011

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The tailor made amino acid β-N-methyl-amino-L-alanine (BMAA) is a neurotoxin produced by cyanobacteria. It has been associated with certain forms of progressive neurodegenerative disease, including sporadic Amyotrophic Lateral Sclerosis and Alzheimer's disease. Some different reports of BMAA in cyanobacterial blooms from lakes, reservoirs, and other water resources have been made by different investigators. We here report the detection of BMAA of both free and protein-bound produced by cyanobacteria, belonging to the Chroococcales, Oscillatoriales and Nostocales ordered. We use a rapid and sensitive HPLC-FD method that utilizes methanol elution and the Waters AQC Tag chemistry. On other hand, we have used three different assay procedures for BMAA extraction from cyanobacteria: Trichloroacetic acid (TCA), Methanol/Acetone and hydrochloric acid (HCl). All assays let successfully detect BMAA in all cyanobacteria samples analyzed. Nevertheless, with TCA and HCl extraction procedures the highest BMAA values, for free as well as protein-bound BMAA were detected. BMAA content could not be related to the taxonomy of the isolates or to their geographical origin, and no correlation between free and protein-bound BMAA concentrations were observed within or between taxonomic groups. These data offer confirmation of the taxonomic and geographic ubiquity of BMAA from naturally occurring populations of cyanobacteria, for the first time reported for estuaries.

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Primary Screening of the Bioactivity of Brackishwater Cyanobacteria: Toxicity of Crude Extracts to Artemia salina Larvae and Paracentrotus lividus Embryos

Cited by 43 publications

References 20 publications

Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

The non-protein amino acid β-N-methylamino-l-alanine in Portuguese cyanobacterial isolates

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