Primary structure of mature SAG1 gene of an Indonesian<i>Toxoplasma gondii</i>and comparison with other strains

Hartati, Sri; Kusumawati, Asmarani; Wuryastuti, Hastari; Widada, Joannes Sri

doi:10.4142/jvs.2006.7.3.263

Cited by 11 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study by Hartati et al (2006) on the SAG1 gene from goat isolate in Indonesia reported a genuine T. gondii RH type I strain, which was similar to the result by Dubey et al (2008) on SAG1 and SAG2 locus from chicken isolates. However, later analysis on 10 genetic markers showed that the chicken isolate was a nonclonal genotype ToxoDB#89, which was only found in Indonesia (Chaichan et al, 2017).…”

Section: Discussionsupporting

confidence: 81%

Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia

Kurniawan

Sari

Harminarti

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

This study aimed to perform genotyping of Toxoplasma gondii strain or variant causing atypical toxoplasmic uveitis in Indonesian patients. Methods: Ocular fluid samples originating from 46 uveitis patients with non-specific ocular manifestations were analysed for Toxoplasma infection by PCR of the B1 locus. The clonal type was determined by amplification, sequencing and phylogenetic analysis of SAG2 and GRA6 loci in B1-positive samples. Clinical data were obtained from the medical records. Results: Pan uveitis was the most frequent manifestation (65.2%) and mostly unilateral (76.1%). PCR of the B1 locus identified eight positive subjects (12.5%); six had panuveitis and two of these had diabetes mellitus. Phylogenetic analysis with maximum likelihood of the SAG2 locus in the B1-positive samples resulted in Toxoplasma gondii SAG2 type III allele. No positive result was obtained from the PCR of GRA6 locus. Conclusion: Toxoplasma gondii SAG2-type III allele was identified in an atypical presentation of toxoplasmic uveitis in Indonesia.

show abstract

Section: Discussionsupporting

confidence: 81%

Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia

Kurniawan

Sari

Harminarti

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…Among the several cloned genes encoding T. gondii antigens, the surface antigen 1 (SAG1) (also named P30) has proved to be a good candidate for serodiagnosis of toxoplasmosis (10,18). In fact, it is a highly conserved antigen in most T. gondii strains examined (19,20,21), is extremely immunogenic, and is recognized during the acute and chronic phases of toxoplasmosis (22,23,24).…”

mentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay Using Recombinant SAG1 Antigen To Detect Toxoplasma gondii-Specific Immunoglobulin G Antibodies in Human Sera and Saliva

Bel-Ochi

Bouratbine

Mousli

2013

Clin Vaccine Immunol

View full text Add to dashboard Cite

Serologic detection of Toxoplasma gondiiIgG antibodies is widely accepted as a means to determine immune status and susceptibility to Toxoplasma infection during pregnancy. However, current commercial kits present some drawbacks, such as a requirement for whole-parasite antigen preparation or interassay variability. To address these problems, the purpose of this study was to produce a whole sequence of the recombinant T. gondii SAG1 antigen (rSAG1) to assess its diagnostic performance in Toxoplasma IgG screening and to explore a saliva-based method as a noninvasive alternative to serum-based testing. rSAG1 was expressed in recombinant bacteria as inclusion bodies, purified through one-step affinity chromatography, and refolded in native form by dialysis. A large amount was obtained, and the specific antigen immunoreactivity was confirmed by immunoblotting. Two rSAG1-based enzyme-linked immunosorbent assays (ELISAs) applied to paired serum and saliva samples were designed. The rSAG1-based ELISA evaluation consisted of testing intrinsic sensitivity and specificity of 49 serum samples from patients immune to toxoplasmosis and 42 serum samples from nonimmune controls identified by routinely used kits. To assess agreement between serum-based and saliva-based tests, the positive percent agreement (PPA) and negative percent agreement (NPA) between the 2 tests were estimated. The rSAG1 serum-based ELISA detected specific IgG with 100% sensitivity and specificity. The PPA and NPA between the serum-based and saliva-based tests varied according to the selected optical density threshold in saliva. Thus, for a selected cutoff of 0.14, the PPA was 100% and the NPA was 88.1%, whereas for a selected cutoff of 0.29, the PPA was 67.3% and the NPA was 100%.

show abstract

“…The SAG1 is a good candidate for serodiagnosis of toxoplasmosis using cloned genes of T. gondii [ 45 , 46 ]. It is an immunodominant antigen highly conserved in nature and used to identify different strains of T. gondii [ 47 , 48 ]. Native SAG1 protein has six intramolecular cysteine bridges, making it an immunodominant antigen with immunologically conserved epitopes.…”

Section: Introductionmentioning

confidence: 99%

Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit

Sarfraz-ur-Rahman

Akbar

Shabbir

et al. 2022

Animals

View full text Add to dashboard Cite

Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis in warm-blooded vertebrates, globally. The main aims of this study were to assess the seropositivity to toxoplasmosis of an exotic breed of cattle (n = 400) from different farms using the Latex Agglutination Test and validate Cattle Toxo IgG ELISA kit. Of a total of 400 cattle sera that were evaluated by LAT, 143 (35.75%) were found positive. Based on these data, 90 samples (n = 60 seronegative by LAT; n = 30 seropositive by LAT) were elected for screening through a commercially available ELISA kit. The same 90 samples were screened through a Cattle Toxo IgG ELISA kit for validation purposes. Of 90 samples, 40 were seropositive in the Cattle Toxo IgG ELISA kit (100% sensitivity), and 38 were seropositive in a commercially available ELISA kit. All 50 samples in the Cattle Toxo IgG ELISA kit (96.15% specificity) were also seronegative in the commercially available ELISA kit. Hence, the sensitivity and specificity of the Cattle Toxo IgG ELISA kit came out to be 100% and 96.15%, and in LAT, it was found as 26.31% and 61.53%, respectively. Therefore, the Cattle Toxo IgG ELISA kit is a highly reliable serodiagnostic tool to diagnose bovine toxoplasmosis.

show abstract

Primary structure of mature SAG1 gene of an IndonesianToxoplasma gondiiand comparison with other strains

Cited by 11 publications

References 16 publications

Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia

Toxoplasma gondii SAG2 type III in an atypical presentation of ocular toxoplasmosis in Indonesia

Enzyme-Linked Immunosorbent Assay Using Recombinant SAG1 Antigen To Detect Toxoplasma gondii-Specific Immunoglobulin G Antibodies in Human Sera and Saliva

Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit

Contact Info

Product

Resources

About