Prime Enzymatic Exocellular Background of &amp;lt;i&amp;gt;Lysinibacillus louembei&amp;lt;/i&amp;gt;

Kaya-Ongoto, Moïse Doria; Kayath, Christian Aimé; Mbozo, Alain Brice Vouidibio; Mitoko, Gyna Mobandolaka; Wilson, Sandra Paola Elenga; Kinavouidi, Duchel Jeanedvi Kinouani; Nguimbi, Etienne

doi:10.4236/aim.2020.103009

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…As per reported by Kaya-Ongoto et al (27) few studies have been shown that Lysinibacillus genera have possesses several enzymatic activities including lipolytic, proteolytic, cellulolytic, pectinolytic and amylolytic activities (16). Many lipase producing bacterial strains were isolated from diverse genera such as Bacillus (the most predominant), Pseudomonas, Serratia, Lysinibacillus, Cronobacter, Cellulosimicrobium (former Oerskovia) and Klebsiella sp, as per studies carried by Caterina Tomulescu et al (28).…”

Section: Discussionmentioning

confidence: 84%

Isolation, Optimization and Molecular Characterization of Novel Lipase Producing Lysinibacillus macroides FS1 from Oil Rich Sample for Biodiesel Production

Jigajinni¹,

Meti²

2021

CTBP

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Section: Discussionmentioning

confidence: 84%

Isolation, Optimization and Molecular Characterization of Novel Lipase Producing Lysinibacillus macroides FS1 from Oil Rich Sample for Biodiesel Production

Jigajinni¹,

Meti²

2021

CTBP

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“…e spore-forming bacteria like B. subtilis, B. lichenifornis, and Lysinibacillus louembei have been widely used to produce BLM [18][19][20].…”

Section: Discussionmentioning

confidence: 99%

“…Gram-positive bacteria are known as being efficient in producing BLM. The spore-forming bacteria like B. subtilis , B. lichenifornis , and Lysinibacillus louembei have been widely used to produce BLM [ 18 – 20 ].…”

Section: Discussionmentioning

confidence: 99%

Invasion of Epithelial Cells Is Correlated with Secretion of Biosurfactant via the Type 3 Secretion System (T3SS) of Shigella flexneri

Kinavouidi

Kayath

Nguimbi

2020

Journal of Pathogens

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Biosurfactants are amphipathic molecules produced by many microorganisms, usually bacteria, fungi, and yeasts. They possess the property of reducing the tension of the membrane interfaces. No studies have been conducted on Shigella species showing the role of biosurfactant-like molecules (BLM) in pathogenicity. The aim of this study is to assess the ability of Shigella environmental and clinical strains to produce BLM and investigate the involvement of biosurfactants in pathogenicity. Our study has shown that BLM are secreted in the extracellular medium with EI24 ranging from 80% to 100%. The secretion is depending on the type III secretion system (T3SS). Moreover, our results have shown that S. flexneri, S. boydii, and S. sonnei are able to interact with hydrophobic areas with 17.64%, 21.42%, and 22.22% hydrophobicity, respectively. BLM secretion is totally prevented due to inhibition of T3SS by 100 mM benzoic and 1.5 mg/ml salicylic acids. P. aeruginosa harboring T3SS is able to produce 100% of BLM in the presence or in the absence of both T3SS inhibitors. The secreted BLM are extractable with an organic solvent such as chloroform, and this could entirely be considered a lipopeptide or polypeptide compound. Secretion of BLM allows some Shigella strains to induce multicellular phenomena like “swarming.”

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“…Gram positive bacteria are known as professional one in the production of BLM. The spore-forming bacteria like B. subtilis, B. lichenifornis and Lysinibacillus louembei have been widely used to produce BLM [39–41].…”

Section: Discussionmentioning

confidence: 99%

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Kinavouidi

Kayath²,

Nguimbi

2020

Preprint

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11Biosurfactants are amphipathic molecules produced by many microorganisms, usually bacteria, 12 fungi and yeasts. They possess the property of reducing the tension of the membrane interfaces. 13 No studies have been conducted on Shigella species showing their involvement of biosurfactant 14 like molecules (BLM) in pathogenicity. This study aims to show that environmental and clinical 15 strains of Shigella are able to produce BLM by emulsifying gasoline and diesel fuels. Our study 16 has shown that BLM are secreted in the extracellular medium with EI24 ranging from 80 to 17 100%. The secretion is depending on the type III secretion system (T3SS). We did show that S. 18 flexneri, S. boydii and S. sonnei are able to interact with hydrophobic areas with respectively 19 17.64%, 21.42% and 22.22% of hydrophobicity. 100 mM Benzoic and 1.5mg/mL Salycilic 20 acids have been inhibited T3SS and this totally stops the BLM secretion. Pseudomonas 21 aeruginosa which has T3SS is able to produce 100% of BLM in the presence or in the absence 22 of both T3SS inhibitors. The secreted BLM is extractable with an organic solvent such as 23 chloroform and could entirely be considered like lipopeptide or polypeptidic compound. By 24 secreting BLM, Shigella is able to perform multicellular phenomena like "swarming" allowing 25 to invade and disseminate inside epithelial cells. 26 Introduction 28 The ingestion of pathogenic and virulent microorganisms generally affecting peoples in both 29 developed and developing countries [1]. Shigella is one of the Gram-negative bacterium 30 belonging to Enterobacteriaceae family and is causative agent of bacillary dysentery or 31 shigellosis [2]. The genus Shigella was the major pathogen bacteria associated with dysentery 32with attributable fraction to 63,8%, but also the second most common pathogen associated with 33 watery diarrhoea with attributable fraction to 12,9% in sub-Saharan Africa and south Asia. 34 Children under 5 years are the most affected. More and more shigellosis is a pathology that 35 both towards neglected diseases but 164300 of death per years have been notified all over the 36 world in 2010. Most deaths occur in sub-Saharan Africa and in south Asia [3-6]. This is include 37 Republic of Congo and surprisingly no epidemiological studies have been conducted in this 38 field. The genus Shigella includes four species (S. flexneri, S. sonnei, S. dysenteriae and S. 39 boydii) [7]. 10 bacteria of S. dysenteriae type 1 and 100 to 180 bacteria of S. flexneri or S. 40 sonnei are enough to produce symptomatic infection [8].41Shigella's pathogenicity is based on a virulence plasmid pWR100 in which the mxi-spa locus 42 encodes the type three secretion system(T3SS) involved in effector production like IpaB, C and 43 D (Translocator and Tip) to invade host cell [9][10][11][12]. A previous study in our laboratory that 44 showed that Shigella sp. isolated from Brazzaville wastewater were able to emulsify 45 hydrocarbon from gasoline and/or diesel fuel [13]. Sachin et al. found the same pr...

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Prime Enzymatic Exocellular Background of <i>Lysinibacillus louembei</i>

Cited by 5 publications

References 27 publications

Isolation, Optimization and Molecular Characterization of Novel Lipase Producing Lysinibacillus macroides FS1 from Oil Rich Sample for Biodiesel Production

Isolation, Optimization and Molecular Characterization of Novel Lipase Producing Lysinibacillus macroides FS1 from Oil Rich Sample for Biodiesel Production

Invasion of Epithelial Cells Is Correlated with Secretion of Biosurfactant via the Type 3 Secretion System (T3SS) of Shigella flexneri

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

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