Some of the authors of this publication are also working on these related projects:Traditional african fermented cereals View project Selection and assessment of the combination between waste of tropical fruits and probiotic strains in the modulation of intestinal microbiota of obese compared to the normal weight using the simulator of the human intestinal microbial ecosystem View project Labia Irène Ivette Ouoba Ouoba-Consulting; London Metropolitan Univesity

Investigation of the microbial diversity of Ntoba Mbodi, an African food made from the alkaline fermentation of cassava leaves, revealed the presence of a Gram-positive, catalase-positive, aerobic, motile and rod-shaped endospore-forming bacterium (NM73) with unusual phenotypic and genotypic characteristics. spacer-PCR profile of the isolate was different from those of closely related bacteria. The cellwall peptidoglycan type was A4a, L-Lys-D-Asp and the major fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C17 : 0 and iso-C17 : 1v10c. The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid, aminolipid, two phospholipids and two unknown lipids. The predominant menaquinones were MK-7 and MK-6.Ribose was the only whole-cell sugar detected. The DNA G+C content was 38 mol%. Based on the results of the phenotypic and genotypic characterization, it was concluded that the isolate represents a novel species of the genus Lysinibacillus, for which the name of Lysinibacillus louembei sp. nov. is proposed. NM73

Investigation of the diversity and safety of the predominant Bacillus pumilus sensu lato and other Bacillus species involved in the alkaline fermentation of cassava leaves for the production of Ntoba Mbodi

Many high-reading journals reject several studies based on the basic aspects of microbiology by forgetting that this could open doors and windows of great scientific discoveries. A new spore-forming bacteria species called Lysinibacillus louembei has been previously discovered in our research unit. This new study aims to assess a real enzymatic machine produced with such bacteria. Using agarose mixed with half skimmed milk, LB medium supplemented with tween 20, Olive oil, egg yolk, cellulose, casein, pectin and starch, we showed that L. louembei has lipolytic, proteolytic (2.8 cm ± 0.1), cellulolytic, amylolytic pectinolytic activities, with percentage ranging from 30% to 80%. This species is able to secrete lipase, thermostable protease up to 60˚C. Cellulase, pectinase and amylase are secreted with more stability between 30˚C and 50˚C with an optimum at 45˚C. The effect of pH was determined after 24 h of incubation at 37˚C and 50˚C. The proteolytic activity is stable at pH 8 at 50˚C.

The aim of this study is to quickly assess the physicochemical parameters of plantain wine and to be acquainted about the microorganisms genera involved in the fermentation. Numbers of fermented beverage are locally produced in several areas of the Republic of Congo by using local materials. The plantain seems to be a fruit of choice in the manufacture of fermented baverage. This study allow to improve the fermentation carried out in a traditional way with pH values around 4.5±0.4. In addition we showed that plantain wine contain more sugar that desert banana by allowing to obtain 16° of alcohol from plantain and 9° from dessert Banana. Finally the plantain wine contain a couple of Mineral composition including Ca, Fe, Cu, K, F and PO3. This wine showed a very interresting profile in terms of beverage preservation