Priming Host Defense Against Biotic Stress by Arbuscular Mycorrhizal Fungi

Gupta, S. C.; Rautela, Pankaj; Maharana, Chandan; Singh, K. P.

doi:10.1007/978-3-319-49724-2_12

Cited by 4 publications

(5 citation statements)

References 43 publications

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“…are in good agreement with the face-centered cubic (fcc) silver lines of the powder data from JCPDS card number 04-0783 (Table S1). However, some other prominent peaks at 27.27 °, 31.66 °, and 45.74 °could be due to the mixed phase of silver nanoparticles with the bioorganic phase (capping agent) or could be the formation of silver oxide in the incubation drying process [41,42]. Here, the average crystallite or grain domain size was calculated using the Debye-Scherrer equation and was found to be 8.54 nm.…”

Section: Effect Of Reaction Time Onmentioning

confidence: 85%

Biosynthesis of Silver Nanoparticles from Rhododendron arboreum for Metal Sensing, Antibacterial Assessment, and Photocatalytic Degradation

Phuyal

Lamichhane

Gupta

et al. 2022

Journal of Nanomaterials

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The nanomaterial industry has focused on green synthetic methods to avoid unpleasant compounds produced during manufacturing, offering eco-friendly, sustainable, and nature-derived alternative methods. In this study, silver nanoparticles (Ag NPs) have been synthesized from an aqueous extract of the leaves of Rhododendron arboreum, where the pH of the reaction mixture is found to be crucial. The reaction progress monitored using the UV-Vis spectrophotometer displayed a strong absorption band at 425 nm at pH 9, suggesting an optimum pH for the synthesis. The Ag NPs thus synthesized were characterized using instrumental techniques. The attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy showed the presence of phytoconstituents in the aqueous extract, which are believed to be responsible for reducing Ag+ ions to Ag NPs and capping agents on its surface for stability. X-ray diffraction (XRD) showed a highly crystalline nature, and energy-dispersive X-ray (EDX) demonstrated the presence of metallic silver. The scanning and transmission electron microscopy (SEM and TEM, respectively) revealed crystalline morphology and monodisperse Ag NPs of sizes ranging from 23 to 41 nm. Furthermore, the metal-sensing activity of biosynthesized Ag NPs was evaluated using various metal ions; they were utilized for highly selective and sensitive colorimetric detection of Hg2+ in an aqueous medium among various metal ion solutions tested with the detection limit of 0.5 mM using the UV-Vis spectrophotometer. Similarly, they were also shown to be effective for the nanocatalytic activity for degradation of methylene blue dye up to 81%. These studies demonstrated Ag NPs as potential candidates for selective detection of mercury in water resources, a tool for sensing the heavy metals and degradation of synthetic dye from industrial effluents in wastewater treatment. Having the high surface-to-volume ratio and size-dependent functionality of Ag NPs, further optimization studies at micromolar and nanomolar detection limits will avail its better industrial utilization. Moreover, biologically mediated Ag NPs can also exhibit good antimicrobial activity against Staphylococcus aureus and Escherichia coli.

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Section: Effect Of Reaction Time Onmentioning

confidence: 85%

Biosynthesis of Silver Nanoparticles from Rhododendron arboreum for Metal Sensing, Antibacterial Assessment, and Photocatalytic Degradation

Phuyal

Lamichhane

Gupta

et al. 2022

Journal of Nanomaterials

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“…AMF symbioses also enhance their host’s tolerance to pathogen pressure at two complementary levels. Fungal root colonisation protects the host both by competing with soil pathogens for its photosynthates and colonisation sites and by triggering a local defence response (accumulation of callose, ROS, phenols and R proteins) ( Schouteden et al., 2015 ; Gupta et al., 2017 ; Dowarah et al., 2021 ). By modulating phytohormonal pathways such as jasmonate, salicylic acid and ethylene, AMF primes its host’s defence responses in the shoot via a mechanism called mycorrhiza-induced resistance (MIR) ( Jung et al., 2012 ; Gupta et al., 2017 ; Fiorilli et al., 2018 ; Nishad et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Fungal root colonisation protects the host both by competing with soil pathogens for its photosynthates and colonisation sites and by triggering a local defence response (accumulation of callose, ROS, phenols and R proteins) ( Schouteden et al., 2015 ; Gupta et al., 2017 ; Dowarah et al., 2021 ). By modulating phytohormonal pathways such as jasmonate, salicylic acid and ethylene, AMF primes its host’s defence responses in the shoot via a mechanism called mycorrhiza-induced resistance (MIR) ( Jung et al., 2012 ; Gupta et al., 2017 ; Fiorilli et al., 2018 ; Nishad et al., 2020 ). This MIR results in reduced foliar symptoms and control of pathogen development on a variety of plants and shoot pathogens ( Liu et al., 2007 ; Fiorilli et al., 2018 ; Kadam et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic compatibility between rice cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance

Guigard,

Jobert,

Busset

et al. 2023

Front. Plant Sci.

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IntroductionArbuscular mycorrhizal fungi (AMF) belong to the Glomeromycota clade and can form root symbioses with 80% of Angiosperms, including crops species such as wheat, maize and rice. By increasing nutrient availability, uptake and soil anchoring of plants, AMF can improve plant’s growth and tolerance to abiotic stresses. AMF can also reduce symptoms and pathogen load on infected plants, both locally and systemically, through a phenomenon called mycorrhiza induced resistance (MIR). There is scarce information on rice mycorrhization, despite the high potential of this symbiosis in a context of sustainable water management in rice production systems.MethodsWe studied the symbiotic compatibility (global mycorrhization & arbuscules intensity) and MIR phenotypes between six rice cultivars from two subspecies (indica: IR64 & Phka Rumduol; japonica: Nipponbare, Kitaake, Azucena & Zhonghua 11) and three AMF genotypes (Funneliformis mosseae FR140 (FM), Rhizophagus irregularis DAOM197198 (RIR) & R. intraradices FR121 (RIN)). The impact of mycorrhization on rice growth and defence response to Xanthomonas oryzae pv oryzae (Xoo) infection was recorded via both phenotypic indexes and rice marker gene expression studies.ResultsAll three AMF genotypes colonise the roots of all rice varieties, with clear differences in efficiency depending on the combination under study (from 27% to 84% for Phka Rumduol-RIN and Nipponbare-RIR combinations, respectively). Mycorrhization significantly (α=0.05) induced negative to beneficial effects on rice growth (impact on dry weight ranging from -21% to 227% on Azucena-FM and Kitaake-RIN combinations, respectively), and neutral to beneficial effects on the extent of Xoo symptoms on leaves (except for Azucena-RIN combination which showed a 68% increase of chlorosis). R. irregularis DAOM197198 was the most compatible AMF partner of rice, with high root colonisation intensity (84% of Nipponbare’s roots hyphal colonisation), beneficial effects on rice growth (dry weight +28% (IR64) to +178% (Kitaake)) and decrease of Xoo-induced symptoms (-6% (Nipponbare) to -27% (IR64)). Transcriptomic analyses by RT-qPCR on leaves of two rice cultivars contrasting in their association with AMF show two different patterns of response on several physiological marker genes.DiscussionOverall, the symbiotic compatibility between rice cultivars and AMF demonstrates adequate colonization, effectively restricting the nutrient starvation response and mitigating symptoms of phytopathogenic infection.

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“…Mycorrhization impacts inorganic phosphate (Pi) responsive genes expression in multiple plant species, such as rice or wheat, suppressing for example the Pi starvation response typically occurring in low P soils ( AMF symbiosis impacts the plant response to phytopathogen infection on two complementary levels. Fungal root colonisation protects the host both by competing with soil pathogens for its photosynthates and colonisation sites, and by triggering a localised defence response (callose, ROS, phenols and R proteins accumulation) (Schouteden et al 2015;Gupta et al 2017;Dowarah et al 2021). By impacting phytohormonal pathways such as jasmonate, salicylic acid and ethylene, AMF symbiosis primes its host's defence responses in shoots, via the mechanism called the Mycorrhiza-Induced Resistance (MIR) (Jung et al 2012;Gupta et al 2017;Fiorilli et al 2018;Nishad et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Fungal root colonisation protects the host both by competing with soil pathogens for its photosynthates and colonisation sites, and by triggering a localised defence response (callose, ROS, phenols and R proteins accumulation) (Schouteden et al 2015;Gupta et al 2017;Dowarah et al 2021). By impacting phytohormonal pathways such as jasmonate, salicylic acid and ethylene, AMF symbiosis primes its host's defence responses in shoots, via the mechanism called the Mycorrhiza-Induced Resistance (MIR) (Jung et al 2012;Gupta et al 2017;Fiorilli et al 2018;Nishad et al 2020). This MIR leads to reduced leaf symptoms and a control of the pathogen development on a variety of plants and shoot pathogens (Liu et al 2007;Fiorilli et al 2018;Kadam et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic compatibility between Oryza sativa cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance to Xanthomonas oryzae pv oryzae

Guigard,

Jobert,

Busset

et al. 2023

Preprint

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Background Arbuscular Mycorrhizal Fungi (AMF) belong to the Glomeromycota clade and can form root symbioses with 80% of Angiosperms, including agronomically-interesting crops species such as wheat, maize and rice. By increasing nutrient availability, uptake and soil anchoring of plants, AMF can improve plant’s growth and tolerance to abiotic stresses. AMF can also reduce symptoms and pathogen load on infected plants, both locally and systemically, through a phenomenon called Mycorrhiza-Induced Resistance (MIR). There is scarce information on rice mycorrhization, despite the high potential of this symbiosis in a context of sustainable water management in rice production systems. Results We studied the symbiotic compatibility (global mycorrhization & arbuscules intensity) and MIR phenotypes between six rice cultivars from two subspecies (indica: IR64 & Phka Rumduol; japonica: Nipponbare, Kitaake, Azucena & Zhonghua 11) and three AMF genotypes (Funneliformis mosseae FR140, Rhizophagus irregularis DAOM197198 & R. intraradices FR121). The impact of mycorrhization on rice growth and defence response to Xanthomonas oryzae pv. oryzae (Xoo) infection was recorded via both phenotypic indexes and rice marker gene expression studies. All three AMF genotypes colonise the roots of all rice varieties, with clear differences in symbiotic compatibility depending on the combination under study. AMF interaction induced either neutral, beneficial, or negative effects on rice growth, but only neutral to beneficial effects on the extent of Xoo symptoms on leaves. R. irregularis DAOM197198 proved to be the most colonising AMF in terms of global mycorrhization and arbuscule intensities, inducing rice growth and reducing symptoms caused by Xoo in all rice varieties. Transcriptomic analyses by RT-qPCR on leaves of two rice cultivars contrasting in their interactions with AMF, shows two different pattern of response both on growth and defence marker genes, that can be related to their phenotypic responses. Conclusions The symbiotic compatibility between rice and AMF depends both on plant cultivar and AMF genotypes. Under our conditions, it drives beneficial, neutral, or negative effects on rice growth, and in some cases, MIR phenotypes after Xoo leaf infection. The interactions between rice and AMF genotypes drive different transcriptomic responses, shedding light on molecular markers of compatibility at the leaf level.

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Priming Host Defense Against Biotic Stress by Arbuscular Mycorrhizal Fungi

Cited by 4 publications

References 43 publications

Biosynthesis of Silver Nanoparticles from Rhododendron arboreum for Metal Sensing, Antibacterial Assessment, and Photocatalytic Degradation

Biosynthesis of Silver Nanoparticles from Rhododendron arboreum for Metal Sensing, Antibacterial Assessment, and Photocatalytic Degradation

Symbiotic compatibility between rice cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance

Symbiotic compatibility between Oryza sativa cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance to Xanthomonas oryzae pv oryzae

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