Principles and applications of luminescence spectrometry coupled to liquid chromatographic techniques

Baeyens, Willy R. G.; Ling, Bowen; Brinkman, U.A.Th.

doi:10.1002/bio.1170040164

Cited by 12 publications

(3 citation statements)

References 52 publications

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“…The applications of luminescence spectrometry in HPLC are numerous and have been reviewed on a regular basis [168]. The use of micelles as an enhancing means in chromatography is not new either [169] but it has seen a strong extension in the recent years.…”

Section: Micelles and Cyclodextrins In Separation Science Using Fluormentioning

confidence: 99%

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Lerner¹,

Martín²

2000

Analusis

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Introduction Molecular luminescence of analytes in organized media or in supramolecular complexes of cyclodextrinsThe photophysical and photochemical behaviour of molecules complexed to cyclodextrins (CD) is generally different from their behaviour in solution. The first observations in the field were qualitative and were related to the increased stabilisation of labile molecules, mostly drugs, against photodegradation when complexed to natural cyclodextrins [1]. Complexation by CD's was then the preferred subject for those involved in supramolecular chemistry and drug vectorisation techniques [2] as well as in separation techniques [3][4][5]. Stabilization of a molecule against photochemical degradation is still very important in the field of pharmaceutical sciences. A recent example is the stabilisation of promazine as an inclusion complex with β-cyclodextrin [6]. Other photochemists were attracted quite early to this field of research and initiated studies, which were mostly photophysical in nature [7,8]. The key observation was that the formation of supramolecular complexes of analytes with cyclodextrins (CDs) resulted in an increase of their fluorescence quantum yield or even in the appearance of room temperature phosphorescence (RTP) [9][10][11][12]. This enhancement has been used to increase the sensitivity and the selectivity in both luminescence and chromatographic techniques [13-15].We will not say more about RTP with CDs as the subject will be developed in another contribution in the same issue of this Journal. However RTP is also observed in the solubilization of analytes in micellar colloïdal solutions and this field will be reviewed hereunder.These properties have been used to develop analytical techniques, which combine the selectivity of complexation with an enhanced emission [16][17][18][19]. In this way an improvement in the detection limits is obtained in many separation techniques such as HPLC, capillary electrophoresis (CE) or planar chromatography. Furthermore these molecular interactions allow to reveal or to stabilize new molecular forms or to turn species which are normally non-emitting into luminescent ones. All these aspects are reviewed hereunder after a short summary on the nature of the intermolecular interactions, which lie at the heart of these effects. Nature of the interactions between an analyte and a CD or a micelleWithout going into a thorough analysis of the origin of the interactions which exist between molecules in dense media which concern us here, it seems useful to recall the most important factors which should be taken into account when any type of luminescence, fluorescence or phosphorescence is involved.

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Section: Micelles and Cyclodextrins In Separation Science Using Fluormentioning

confidence: 99%

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Lerner¹,

Martín²

2000

Analusis

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“…As a general rule (which rule is expected to show exceptions in the course of further research), it should be feasible to chemically excite various fluorescent species, including native fluorescers or fluorescent derivatives generated after suitable labeling reactions as described for various liquid chromatographic [17][18][19][20][21][22][23][24] and CE [25] separations.…”

Section: Figure 1 Aryl Oxalate-hydrogen Peroxide Reaction Producing mentioning

confidence: 99%

Chemiluminescence detection in capillary electrophoresis

Baeyens

Ling

Imai

et al. 1994

J Microcolumn Separations

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Abstract. Capillary electrophoresis techniques offer high plate numbers and are highly suited for the efficient separation of a wide variety of chemical components in diverse matrices. Because of the small capillary and detection cell dimensions, together with the minute volumes of samples to be injected, sensitive detection devices based on different physicochemical principles are being developed by various scientific groups and companies. The nature, possible applications and limitations of chemiluminescence as a detection techmque areoutlined in the present manuscript. The technique so far has only been explored by a limited number of research groups, and serious developments are to be expected for the coming years.

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“…This compound can also be used to treat congestive heart failure [2]. Although several analytical methods, including spectrophotometry [3], chromatography [4][5][6][7][8] and capillary zone electrophoresis (CZE) [9], have been reported for the quantitative determination of captopril, these methods suffer from poor sensitivity, time-consuming and requiring expensive instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Flow Injection Chemiluminescence Determination of Captopril with In Situ Electrogenerated Mn3+ as the Oxidant

Zheng

Zhang

2001

Electroanalysis

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Principles and applications of luminescence spectrometry coupled to liquid chromatographic techniques

Abstract: An overview is presented of the physicochemical basis of luminescence, and its application to the detection of chemicals (drugs, biomedically important compounds, environmentally active substances) in liquid chromatographic systems.

Cited by 12 publications

References 52 publications

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Chemiluminescence detection in capillary electrophoresis

Flow Injection Chemiluminescence Determination of Captopril with In Situ Electrogenerated Mn3+ as the Oxidant

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