Principles and applications of polymerase chain reaction in medical diagnostic fields: a review

Valones, Marcela Agne Alves; Guimarães, Rafael Lima; Brandão, Lucas André Cavalcanti; Souza, Paulo Roberto Eleutério de; Carvalho, Alessandra de Albuquerque Tavares; Crovela, Sergio

doi:10.1590/s1517-83822009000100001

Cited by 149 publications

(98 citation statements)

References 108 publications

(115 reference statements)

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“…Murex LA (Monoclonal IgM based), eliminates false positive, but has lower sensitivity than LA (Poly IgG). In EIA -sensitivity and specificity similar to LA (Poly IgG), few false positive, not react with rheumatoid factor; also multiple samples can be tested [15].…”

Section: Detection Of Capsular Antigen In Cryptococcosismentioning

confidence: 99%

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Recent Diagnostic Techniques in Mycology

P¹

2014

J Med Microb Diagn

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The frequency of fungal infections, especially of Invasive Fungal Infections (IFIs), has risen dramatically in recent years, and has high morbidity and mortality. Early and accurate diagnosis is important for timely implementation of antifungal therapy and decreasing the unnecessary use of toxic antifungal agents. Conventional microbiological, histological and radiological techniques remain the cornerstone of diagnosis but are insensitive and have a limited impact on clinical decision-making. There is always a need to characterize fungi quickly and accurately which can be done by rapid diagnostic techniques like antigen detection and molecular techniques. This is a brief review of all the microbiological techniques-conventional and recent alternative techniques especially elaborating antigen detection and molecular techniques which are available for diagnosis of fungal infections.

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Section: Detection Of Capsular Antigen In Cryptococcosismentioning

confidence: 99%

“…It is 10-fold more sensitive than detection by ethidium bromide-stained agarose gel. TaqMan assays are available to detect isolates of Candida species and Aspergillus fumigates [15].…”

Section: Real Time Pcrmentioning

confidence: 99%

Recent Diagnostic Techniques in Mycology

P¹

2014

J Med Microb Diagn

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“…1 This standard amplification technique can be applied for various biological studies such as genome sequencing, molecular genetic analysis, diagnosis of infectious disease, and genetic disorder. 2,3 Even though PCR is an efficient gene-amplification system, the expensive device, requirement of a trained person, long turnaround times, non portability, and difficulties in the quantification of end products are considered to be limitations of the real-time diagnosis field. 4 As a result, real-time PCR and quantitative PCR systems have been developed to both amplify and detect a DNA simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Real-time DNA Amplification and Detection System Based on a CMOS Image Sensor

Wang

Devadhasan

Lee³

et al. 2016

ANAL. SCI.

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In the present study, we developed a polypropylene well-integrated complementary metal oxide semiconductor (CMOS) platform to perform the loop mediated isothermal amplification (LAMP) technique for real-time DNA amplification and detection simultaneously. An amplification-coupled detection system directly measures the photon number changes based on the generation of magnesium pyrophosphate and color changes. The photon number decreases during the amplification process. The CMOS image sensor observes the photons and converts into digital units with the aid of an analog-to-digital converter (ADC). In addition, UV-spectral studies, optical color intensity detection, pH analysis, and electrophoresis detection were carried out to prove the efficiency of the CMOS sensor based the LAMP system. Moreover, Clostridium perfringens was utilized as proof-of-concept detection for the new system. We anticipate that this CMOS image sensor-based LAMP method will enable the creation of cost-effective, label-free, optical, real-time and portable molecular diagnostic devices.

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“…Polymerase chain reaction (PCR) offers a fast, reliable, and economical alternative to histology and culture, and is quickly becoming the most widely used method for detecting human, animal, and plant pathogens. 11,15,19,21,23 The current study describes a newly developed PCR-based test to detect the WNS-associated fungus G. destructans directly from bat wing tissue within hours. Reliability of the PCR method was assessed by comparisons with culture and histology techniques.…”

Section: Introductionmentioning

confidence: 99%

Rapid Polymerase Chain Reaction Diagnosis of White-Nose Syndrome in Bats

Lorch

Gargas²,

Meteyer

et al. 2010

J VET Diagn Invest

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Abstract. A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.

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Principles and applications of polymerase chain reaction in medical diagnostic fields: a review

Cited by 149 publications

References 108 publications

Recent Diagnostic Techniques in Mycology

Recent Diagnostic Techniques in Mycology

Real-time DNA Amplification and Detection System Based on a CMOS Image Sensor

Rapid Polymerase Chain Reaction Diagnosis of White-Nose Syndrome in Bats

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