Principles of genetic circuit design

Brophy, Jennifer A N; Voigt, Christopher A.

doi:10.1038/nmeth.2926

Cited by 828 publications

(812 citation statements)

References 228 publications

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“…Regulatory pathways in bacterial and mammalian cells have been developed using transcriptional regulatory systems on the basis of CRISPR-Cas9. The progress made in transcriptional regulation by CRISPR allows multiple genes to be set simultaneously [22,23].…”

Section: Examples Of the Use Of The Crispr Techniquementioning

confidence: 99%

CRISPR genome editing and its medical applications

Mahmoudian-Sani

Farnoosh

Mahdavinezhad

et al. 2017

Biotechnology & Biotechnological Equipment

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Genome editing has always been a challenging area as a means to provide more efficient ways to create a meaningful change in the genome. Today, the CRISPR (clustered regularly interspaced short palindromic repeat) restoration system is considered as one of the suitable and promising options for genome editing. This system has many advantages compared to the previous geneediting methods developed in this area. Compared to the previous systems, CRISPR can deactivate or eliminate a gene without interfering with intracellular mechanisms. The system can be used in the treatment of diseases and in related research with identifying the performance of defective genes in these diseases. CRISPR has more potentials and applications compared to previous systems. Among these applications, we can note the use of CRISPR in understanding genetic and epigenetic diseases such as cancer. Study of cancer by the CRISPR system is done by two approaches: turning off the oncogenes and turning on the tumour suppressor genes. According to the exact capability of CRISPR, this system can also be used to create exact mutations in different cell lines to model the cancers. This type of modeling can lead to a better understanding of cancer and the ability to develop effective drugs.

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Section: Examples Of the Use Of The Crispr Techniquementioning

confidence: 99%

CRISPR genome editing and its medical applications

Mahmoudian-Sani

Farnoosh

Mahdavinezhad

et al. 2017

Biotechnology & Biotechnological Equipment

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show abstract

“…The sixth principal component mainly integrates the information of platelet and leukocyte, and the former is in the negative direction of the sixth principal component. The platelet is produced from a giant cell, and the giant cell and leukocyte are both belong to the human body cells, so we name 6 f as the cell factor. These six factors are set as the input of the next level of acute stroke screening process, and the results of the test will verify the rationality of the combination of selected biological markers.…”

Section: 3total Variance Explained Of Pca(principal Component Analymentioning

confidence: 99%

“…Compared to the traditional detection methods which usually have considerable experimental requirements and costs, markers can not only explore the pathogenesis at the molecular level, but also have unique advantages in accurate and sensitive evaluation of early, low levels damage, which provides an early warning of the disease [4]. Many markers such as, s100 and GFAP, have been found to play important roles in the diagnosis, treatment and prognosis of stroke [5][6]. Thus, taking advantage of the genetic circuits inside cells, we can achieve more sensitive and economical marker detection with good real-time performance.…”

Section: Introductionmentioning

confidence: 99%

Optimised Selection of Stroke Biomarker Based on Svm and Information Theory

et al. 2017

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Abstract-With the development of molecular biology and gene-engineering technology, gene diagnosis has been an emerging approach for modern life sciences. Biological marker, recognized as the hot topic in the molecular and gene fields, has important values in early diagnosis, malignant tumor stage, treatment and therapeutic efficacy evaluation. So far, the researcher has not found any effective way to predict and distinguish different type of stroke. In this paper, we aim to optimize stroke biomarker and figure out effective stroke detection index based on SVM (support vector machine) and information theory. Through mutual information analysis and principal component analysis to complete the selection of biomarkers and then we use SVM to verify our model. According to the testing data of patients provided by Xuanwu Hospital, we explore the significant markers of the stroke through data analysis. Our model can predict stroke well. Then discuss the effects of each biomarker on the incidence of stroke.

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“…Researchers now have already constructed gate circuits, latch, and inverting amplifier in the gene circuit to build a complex regulation network to simulate the biochemical reactions in organisms [9][10]. Collins constructed a genetic toggle switch containing promoter that drives the expression of mutually inhibitory transcriptional repressors [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic Circuit for the Early Warning of Lung Cancer using iBioSim

Wang¹,

Wang²,

Jiang³

et al. 2016

ITM Web Conf.

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Abstract. With the development of molecular biology and gene-engineering technology, gene diagnosis has been an emerging approach for modern life sciences. Biological marker, recognized as the hot topic in the molecular and gene fields, has important values in early diagnosis, malignant tumour stage, treatment and therapeutic efficacy evaluation. The design of markers detection genetic circuit system for lung cancer is presented as a new method to provide basis for early warning and therapy. The system consists of three singlemarker detection circuits and an integration circuit. The single-marker detection circuit provides an instantaneous low level when target marker's concentration reaches the threshold. The integration circuit uses gene and gate to complete the output data fusion from single-marker detection circuit through logic operations to finish the combined detection. All the structure is modelled and analyzed by iBioSim through the biochemical reactions of different gene circuits. The experimental result indicates that the whole lung cancer detection system can realize joint detection of tumor markers with good stability and sensitivity.

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Principles of genetic circuit design

Cited by 828 publications

References 228 publications

CRISPR genome editing and its medical applications

CRISPR genome editing and its medical applications

Optimised Selection of Stroke Biomarker Based on Svm and Information Theory

Genetic Circuit for the Early Warning of Lung Cancer using iBioSim

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