Principles of Hyperspectral Microscope Imaging Techniques and Their Applications in Food Quality and Safety Detection: A Review

Abstract

Hyperspectral imaging (HSI) techniques play an important role in the food industry for providing rapid, nondestructive, and chemical‐free detection method, whereas a microscope can provide detailed information about the microstructure of a food item. As an emerging imaging spectroscopy technique, hyperspectral microscope imaging (HMI) technique combines the advantages of HSI with microscopic imaging and has been gradually applied in the food industry. This review introduces the principles of different kinds of HMI techniques, such as fluorescence HMI, visible/near‐infrared HMI, Raman HMI, and infrared HMI. Moreover, detailed applications of HMI techniques are summarized, including evaluation of structures of nutrients, and detection of microorganisms and residues. On the other hand, some challenges and future trends in the applications of these techniques are also discussed. It is concluded that by integrating HSI with microscopy, HMI can not only provide both spectral and spatial information about food substances but also provide their chemical information at the molecular or cellular level. Therefore, HMI techniques have great potentials in nondestructive evaluation of structures of nutrients, and detection of microorganisms and residues for the food industry.

Characterizing Dielectric Permittivity of Nanoscale Dielectric Films by Electrostatic Micro-Probe Technology: Finite Element Simulations

“…The principle of these microscopic techniques have similar probe structures which characterize materials by scanning on the specimen surface with a needle tip to obtain information such as micromorphology, charge distribution, impurity state, dielectric permittivity, etc. [5]. It has become a key research field to quantify the dielectric permittivity of nanoscale objects by the scanning probe microscope.…”

Study on the Relations between Hyperspectral Images of Bananas (Musa spp.) from Different Countries, Their Compositional Traits and Growing Conditions

“…In the last ten years, HSI technology has been widely used in food authenticity and fraud research, especially adulteration, agricultural product traceability, organic food identification and other related fields [ 48 ]. However, most articles mainly tend to use hyperspectral techniques to accurately identify sample differences, but few works of literature report the causes of these differences.…”

Dark‐field hyperspectral imaging for label free detection of nano‐bio‐materials

“…Combining multivariate data analysis and hyperspectral nano‐FTIR imaging provides chemical and compositional maps of nanomaterials, together with the detection of localized nanoscale chemical interaction with future applications ranging from biomedical imaging to material science (Amenabar et al, 2017; Baker et al, 2014; Wrobel et al, 2019). Some examples of nano‐FTIR imaging include characterization of molecular vibrations within polymer and protein nanostructures (Amenabar et al, 2013), single viruses (Brehm, Taubner, Hillenbrand, & Keilmann, 2006), individual amyloid fibrils (Paulite et al, 2011), monitoring food quality (Pu, Lin, & Sun, 2019; Su & Sun, 2018), analysis of live cells, and classification of cancer in tissue samples among other applications (Baker et al, 2014).…”