Principles of Micellar Electrokinetic Capillary Chromatography Applied in Pharmaceutical Analysis

Hancu, Gabriel; Simon, Brigitta; Rusu, Aura; Mircia, Eleonora; Gyéresi, Árpád

doi:10.5681/apb.2013.001

Cited by 23 publications

(12 citation statements)

References 34 publications

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“…Most pharmaceutical compounds are neutral so the basic CZE (capillary zone electrophoresis) is not suited for their separation because it separates analytes based on differences in their electrophoretic mobilities only. MEKC, a mode of CE, separates neutral compounds as it is based on differential partitioning, like traditional chromatography, between a mobile aqueous phase and a micellar pseudo-stationary phase ( 23 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative Greenness Metric Estimates for Content Uniformity Testing of Anti-Cov-2, GS-5734 in Commercial Vials: Validated Micellar Electrokinetic Chromatographic Assay

Elonsy

Kamal

Hamdy

et al. 2022

Journal of AOAC INTERNATIONAL

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Background The antiviral drug GS-5734 “Remdesivir” is a new phosphoramidate prodrug developed initially as treatment for Ebola virus and then proved to have antiviral properties against other viruses. After clinical trials, it was the first antiviral to be approved by the U.S. Food and Drug Administration in 2020 to treat severe corona virus (COVID-19) cases. The widespread of the current pandemic made an urge to its fast production and marketing. Thus, new analytical methods must be available for its analysis in fast and easy manner with low cost to be applicable in all laboratories. Objective In the current study, a green and economic Micellar Electrokinetic Chromatographic (MEKC) method is proposed for remdesivir analysis. Method Fused-silica capillary (58.5 cm × 50 μm id, 50 cm effective length) with 20 mM borate buffer (pH = 9) and 25 mM sodium dodecyl sulfate was used under positive potential = 30 KV at 25 °C and detection at 245 nm. Results Remdesivir analysis was achieved in approximately 5 min. The method proved to be linear in range of 1–50 μg/mL with correlation coefficient, r > 0.999. Conclusion The MEKC method proposed was applied for the analysis of Remdesivir in its commercial vials. The method was validated per International Conference on Harmonization (ICH) guidelines. Highlights Green chemistry has been the focus of analytical community in the past few years. This method is considered green due to its low energy and solvent consumption without scarifying the method’s sensitivity or selectivity. The method’s green profile has been assessed by different greenness assessment scales to ensure the method is eco-friendly and can be used in pharmaceutical industry.

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Section: Resultsmentioning

confidence: 99%

Comparative Greenness Metric Estimates for Content Uniformity Testing of Anti-Cov-2, GS-5734 in Commercial Vials: Validated Micellar Electrokinetic Chromatographic Assay

Elonsy

Kamal

Hamdy

et al. 2022

Journal of AOAC INTERNATIONAL

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show abstract

“…The traditional CZE method, which focuses on disparities in analyte electrophoretic mobilities, is ineffective for separating neutral components in TCM, as these components move toward the detector at the same rate as the EOF. MEKC is an electrophoretic technique that expands the application of CE to neutral components that cannot be analyzed by free solution CE (Hancu et al, 2013). As discussed in Section 2, MEKC differs from CZE because it uses an ionic micellar solution instead of a simple buffer salt solution.…”

Section: Mekc Analysis Of Various Tcmsmentioning

confidence: 99%

Advances and strategies for capillary electrophoresis in the characterization of traditional Chinese medicine: A review of the past decade (2011–2021)

Shamsi

Patel²

2023

Front. Anal. Sci.

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While traditional Chinese medicine (TCM) is considered a valuable resource for drug discovery and form a potential basis for drug development, they also carry substantial safety risks due to adverse drug reactions and a lack of understanding of their mechanisms of action. However, due to their highly complex composition, valid analytical methodologies for analyzing TCMs must be developed and promoted. An extensive search of published research and review of scientific papers implies that the increased efficiency and sensitivity of capillary electrophoresis (CE) has attracted much research attention. This review provides an in-depth assessment of CE applications for TCM analysis published in the open literature in the last decade (2011–2021). Our survey findings showed that capillary zone electrophoresis (CZE) with ultraviolet (UV) detection is a capillary electromigration technique frequently utilized for the efficient separation, identification, and quantitation of various active components in highly complex matrices. Different extraction methods, modifiers to the background electrolyte, preconcentration techniques, and mass spectrometry (MS) detectors are used to enhance CE separation selectivity and TCM sensitivity.

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“…The migration time of the analytes will depend on the affinity to the micelles. The analytes that have more affinity will have a slow velocity of migration, while the other analytes will migrate faster, and the retention time will be lower [51]. One of the first studies exploiting MEKC to separate AgNPs from wound dressing, for example, was developed in 2019 by M. Konop et al [50].…”

Section: Electrophoresismentioning

confidence: 99%

From Impure to Purified Silver Nanoparticles: Advances and Timeline in Separation Methods

2021

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AgNPs have exceptional characteristics that depend on their size and shape. Over the past years, there has been an exponential increase in applications of nanoparticles (NPs), especially the silver ones (AgNPs), in several areas, such as, for example, electronics; environmental, pharmaceutical, and toxicological applications; theragnostics; and medical treatments, among others. This growing use has led to a greater exposure of humans to AgNPs and a higher risk to human health and the environment. This risk becomes more aggravated when the AgNPs are used without purification or separation from the synthesis medium, in which the hazardous synthesis precursors remain unseparated from the NPs and constitute a severe risk for unnecessary environmental contamination. This review examines the situation of the available separation methods of AgNPs from crude suspensions or real samples. Different separation techniques are reviewed, and relevant data are discussed, with a focus on the sustainability and efficiency of AgNPs separation methods.

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Principles of Micellar Electrokinetic Capillary Chromatography Applied in Pharmaceutical Analysis

Cited by 23 publications

References 34 publications

Comparative Greenness Metric Estimates for Content Uniformity Testing of Anti-Cov-2, GS-5734 in Commercial Vials: Validated Micellar Electrokinetic Chromatographic Assay

Comparative Greenness Metric Estimates for Content Uniformity Testing of Anti-Cov-2, GS-5734 in Commercial Vials: Validated Micellar Electrokinetic Chromatographic Assay

Advances and strategies for capillary electrophoresis in the characterization of traditional Chinese medicine: A review of the past decade (2011–2021)

From Impure to Purified Silver Nanoparticles: Advances and Timeline in Separation Methods

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