Printable and flexible macroporous organosilica film with high protein adsorption capacity

Heikkinen, Jarkko J.; Kivimäki, Liisa; Hytönen, Vesa P.; Kulomaa, Markku S.; Hormi, Osmo

doi:10.1016/j.tsf.2011.09.041

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Therefore, the surface area and pore volume can be wasted as a result of block the pore. Macroporous supports that possess larger pores and higher pore volumes showed better capacity to immobilize biomolecules with higher molecular weight, therefore these supports are good candidates for the immobilization of large biomolecules .…”

Section: Introductionmentioning

confidence: 99%

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes

Sun

Jin

Jiang

et al. 2017

Biotech and App Biochem

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A zinc oxide (ZnO) nanowires/macroporous silicon dioxide composite was used as support to immobilize horseradish peroxidase (HRP) simply by in situ cross-linking method. As cross-linker was adsorbed on the surface of ZnO nanowires, the cross-linked HRP was quite different from the traditional cross-linking enzyme aggregates on both structure and catalytic performance. Among three epoxy compounds, diethylene glycol diglycidyl ether (DDE) was the best cross-linker, with which the loading amount of HRP with pI of 5.3 reached as high as 118.1 mg/g and specific activity was up to 14.9 U/mg-support. The mass loss of HRP cross-linked with DDE was negligible during 50-H leaching at 4 °C, and the thermal stability of the immobilized HRP was also quite good. The catalytic performance of immobilized HRP to decolorize anthraquinone dye was explored by using Reactive Blue 19 (RB 19) and Acid Violet 109 (AV 109) as model substrates. The results indicated that the immobilized HRP exhibited high decolorization efficiency and good reusability. The decolorization efficiency reached 94.3% and 95.9% for AV 109 and RB 19 within the first 30 Min, respectively. A complete decolorization of these two dyes has been realized within 2-3 H by using this new biocatalysis system.

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Section: Introductionmentioning

confidence: 99%

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes

Sun

Jin

Jiang

et al. 2017

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

The nano building blocks approach for modification of surface properties of nanocomposite inorganic–organic films

Pagáčová¹,

Papučová²,

Plško

et al. 2022

J Coat Technol Res

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Confining copper nanoclusters on exfoliation-free 2D boehmite nanosheets: Fabrication of ultra-sensitive sensing platform for α-glucosidase activity monitoring and natural anti-diabetes drug screening

Chen

et al. 2021

Biosensors and Bioelectronics

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Printable and flexible macroporous organosilica film with high protein adsorption capacity

Cited by 3 publications

References 18 publications

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes

The nano building blocks approach for modification of surface properties of nanocomposite inorganic–organic films

Confining copper nanoclusters on exfoliation-free 2D boehmite nanosheets: Fabrication of ultra-sensitive sensing platform for α-glucosidase activity monitoring and natural anti-diabetes drug screening

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Printable and flexible macroporous organosilica film with high protein adsorption capacity

Cited by 3 publications

References 18 publications

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO2 composites for the complete decolorization of anthraquinone dyes

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO2 composites for the complete decolorization of anthraquinone dyes

The nano building blocks approach for modification of surface properties of nanocomposite inorganic–organic films

Confining copper nanoclusters on exfoliation-free 2D boehmite nanosheets: Fabrication of ultra-sensitive sensing platform for α-glucosidase activity monitoring and natural anti-diabetes drug screening

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Product

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Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes

Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO₂ composites for the complete decolorization of anthraquinone dyes