Prior exposure to ciprofloxacin disrupts intestinal homeostasis and predisposes ayu (&lt;i&gt;Plecoglossus altivelis&lt;/i&gt;) to subsequent &lt;i&gt;Pseudomonas plecoglossicida&lt;/i&gt;-induced infection

Wu, Xiangyu; Xiong, Jinbo; Fei, Chen-Jie; Dai, Ting; Zhu, Ting-Fang; Zhao, Zi-Yue; Pan, Jing; Nie, Li; Chen, Jiong

doi:10.24272/j.issn.2095-8137.2022.159

Cited by 10 publications

(7 citation statements)

References 77 publications

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“…Hu et al [6] believed that there are five major hazards of the abuse of antibiotics through comprehensive analysis, the first is the harm to patients. Renal toxicity-The abuse of antibiotics can induce drug-induced renal failure, manifested as proteinuria, hematuria, etc., and in severe cases, azotemia can occur, resulting in decreased renal function [7] ; hepatotoxicity-Drugs cause liver damage through direct damage or allergic mechanisms; the abuse of antibiotics can destroy the normal bacteria in the human body, leading to a double infection; ototoxicity-The abuse of antibiotics can damage to vestibular function and auditory nerves, resulting in vision loss, nystagmus, nausea, vomiting and ataxia, hearing loss and even permanent deafness [8] ; gastrointestinal reaction-Nausea, vomiting, abdominal pain, diarrhea and constipation reactions, toxic reactions, low immunity [9] ; blood toxicity-Red blood cells, white blood cells, platelet blood reduction and even cause hemolytic anemia, aplastic anemia, etc., [10] ; Secondly, the abuse of antibiotics can lead to the emergence of super-resistant bacteria; thirdly, the abuse of antibiotics poses a threat to clinical practice; fourth, the abuse of antibiotics wastes resources; fifth, the antibiotics exist in sewage in an active form, destroying the ecological balance. The abuse of antibiotics is particularly serious in China [11] .…”

Section: Resultsmentioning

confidence: 99%

Effect of Perioperative Antibiotics on Prognosis of Patients with Hemorrhoids

Chen¹,

Xin²,

Hu³

2022

IJPS

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To explore the feasibility of not using antibiotics before and after hemorrhoid surgery is the main objective of the study. A total of 68 patients with hemorrhoids who were admitted to Ningbo Anorectal Hospital from December 2019 to May 2021 were selected, including 36 males and 32 females. They were randomly divided into an experimental group and a control group, 34 patients in each group. The patients in the experimental group were not given antibiotics, while the patients in the control group were given cefazolin sodium 1-2 g and 100 ml of sodium chloride intravenously 30 min before surgery and the same dose of antibiotics was given intravenously after surgery (1 time/12 h), the postoperative administration time is 24 h, not more than 48 h. The intraoperative conditions, postoperative wound infection rate, hospital stay, postoperative recurrence rate and other aspects of the two groups of patients were observed and compared. There was no significant difference in postoperative body temperature, white blood cell count, postoperative wound infection and average hospital stay and recovery time between the two groups. The adverse drug reaction rate in the experimental group was lower than that in the control group. It is safe and feasible not to use antibiotics in the perioperative period of hemorrhoids.

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Section: Resultsmentioning

confidence: 99%

Effect of Perioperative Antibiotics on Prognosis of Patients with Hemorrhoids

Chen¹,

Xin²,

Hu³

2022

IJPS

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“…The total RNA was extracted from the cells using RNAiso reagent (TaKaRa, Dalian, China), and first-strand cDNA was synthesized using AMV reverse transcriptase (TaKaRa, Dalian, China) [ 26 ]. The qRT-PCR was performed on a QuantStudio™3 Real-Time PCR System (Thermo Fisher Scientific Inc., Waltham, MA, USA) using FastStart Essential DNA Green Master (Roche Molecular Diagnostics, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Cultivation for Glugea plecoglossi (Microsporidia) Isolated from Ayu (Plecoglossus altivelis)

Xu,

Zhang,

Zhou

et al. 2024

Microorganisms

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Glugea plecoglossi is an obligate intracellular microsporidium, which poses a significant threat to ayu (Plecoglossus altivelis). In vitro cultivation models are invaluable tools for investigating intracellular microorganisms, including G. plecoglossil. In this study, we attempted to in vitro cultivate G. plecoglossi using primary cultures derived from ayu monocytes/macrophages (MO/MΦ), a murine-derived macrophage cell line RAW264.7, and the epithelioma papulosum cyprini (EPC) cell line. The results demonstrated that MO/MΦ infected with spores exhibited a pronounced immune response which was presented by rapidly high expression levels of inflammatory cytokines, such as PaIL-1β, PaTNF-α, PaIL-10, and PaTGF-β, and detached within 96 h post-infection (hpi). Infected RAW264.7 cells remained capable of stable passage yet exhibited cellular deformation with a decrease in intracellular spores occurring around 8 days post-infection (dpi). In contrast, EPC cells promised a substantial parasite population, and the cytokine expression levels returned to normal by 8 dpi. In addition, G. plecoglossi spores recovered from EPC cells could infect young ayu, suggesting that EPC cells might be used as an in vitro cultivation system for G. plecoglossi.

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“…Histopathological examination was performed as in our previous study [ 21 ]. Three fish from each group were randomly selected at 48 h post infection.…”

Section: Methodsmentioning

confidence: 99%

Oral Vaccination of Recombinant Saccharomyces cerevisiae Expressing ORF132 Induces Protective Immunity against Cyprinid Herpesvirus-2

et al. 2023

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Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis (HVHN) disease, which causes serious economic losses in the crucian carp culture industry. In this study, by displaying ORF132 on the surface of Saccharomyces cerevisiae cells (named EBY100/pYD1-ORF132), we evaluated the protective efficacy of oral administration against CyHV-2 infection. Intense innate and adaptive immune responses were evoked in both mucosal and systemic tissues after oral vaccination with EBY100/pYD1-ORF132. Importantly, oral vaccination provided significant protection for crucian carp post CyHV-2 infection, resulting in a relative percent survival (RPS) of 64%. In addition, oral administration suppressed the virus load and relieved histological damage in selected tissues. Our results indicated that surface-displayed ORF132 on S. cerevisiae could be used as potential oral vaccine against CyHV-2 infection.

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Prior exposure to ciprofloxacin disrupts intestinal homeostasis and predisposes ayu (<i>Plecoglossus altivelis</i>) to subsequent <i>Pseudomonas plecoglossicida</i>-induced infection

Cited by 10 publications

References 77 publications

Effect of Perioperative Antibiotics on Prognosis of Patients with Hemorrhoids

Effect of Perioperative Antibiotics on Prognosis of Patients with Hemorrhoids

In Vitro Cultivation for Glugea plecoglossi (Microsporidia) Isolated from Ayu (Plecoglossus altivelis)

Oral Vaccination of Recombinant Saccharomyces cerevisiae Expressing ORF132 Induces Protective Immunity against Cyprinid Herpesvirus-2

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