Prioritizing of bacterial infections transmitted through substances of human origin in Europe

Domanović, Dragoslav; Cassini, Alessandro; Bekeredjian‐Ding, Isabelle; Bokhorst, Arlinke; Bouwknegt, Martijn; Facco, Giuseppina; Galea, George; Grossi, Paolo; Jashari, Ramadan; Jungbauer, Christoph; Marcelis, J. H.; Raluca-Siska, Ioana; Andersson-Vonrosen, Inger; Suk, Jonathan E.

doi:10.1111/trf.14036

Cited by 16 publications

(13 citation statements)

References 17 publications

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“…5 In a recent study from the European Center for Disease Prevention and Control, E. coli ranks fourth among bacteria and second among Gram-negative bacteria. 35 In comparison, other Gram-negative bacteria as Serratia sp. ranked fourth in France and 10th in Europe.…”

Section: Discussionmentioning

confidence: 99%

“…In France E. coli represented the third bacterium and the first Gram‐negative bacterium responsible for TTBI . In a recent study from the European Center for Disease Prevention and Control, E. coli ranks fourth among bacteria and second among Gram‐negative bacteria . In comparison, other Gram‐negative bacteria as Serratia sp.…”

Section: Discussionmentioning

confidence: 99%

“…ranked fourth in France and 10th in Europe. 35 The genetic backbone of each E. coli strain can be established by determining its phylogenetic group, and most of the virulent lineages belong to the phylogenetic group B2. 13,36 Beyond phylogroups, the genetic diversity of E. coli is substantial.…”

Section: Discussionmentioning

confidence: 99%

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Genotypic and phenotypic characteristics of Escherichia coli involved in transfusion‐transmitted bacterial infections: implications for preventive strategies

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genotypic and phenotypic characteristics of Escherichia coli involved in transfusion‐transmitted bacterial infections: implications for preventive strategies

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“…A ranking of transfusion-transmitted bacteria considering several risk factors showed that all relevant pathogens except for Clostridium spp. can be cultivated under aerobic culture conditions [38]. In Europe, the screening of PC using a single aerobic bottle is implemented in Denmark (Fig.…”

Section: Anaerobic Screening – Is It Worth the Effort?mentioning

confidence: 99%

Microbiological Screening of Platelet Concentrates in Europe

Prax

Bekeredjian‐Ding

Krut³

2019

Transfus Med Hemother

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The risk of transfusion-associated sepsis due to transmission of bacteria is a persistent problem in the transfusion field. Despite numerous interventions to reduce the risk, cases of bacterial sepsis following transfusion are repeatedly being reported. Especially platelet concentrates are highly susceptible to bacterial contaminations due to the growth-promoting storage conditions. In Europe, blood establishments and national authorities have implemented individual precaution measures to mitigate the risk of bacterial transmission. To obtain an overview of the different approaches, we compiled information from national authorities, blood establishments, and the current literature. Several aspects such as the shelf life of platelets, time of sampling and the applied control measures are compared between the member states. The analysis of the data revealed a broad heterogeneity of procedures on a national level ranging from platelet release without any safety testing up to mandatory screening of all platelet concentrates prior to transfusion. Despite the substantial progress made in recent years, several bacterial reports on transfusion-associated sepsis indicate that further efforts are needed to increase the safety of blood transfusions in the long term.

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“…The residual risk of transmitting the major viruses hepatitis C virus, hepatitis B virus, and human immunodeficiency virus has been reduced to a minimum using nucleic amplification technologies and fourth‐generation serology screening tests . In the period 2010 to 2013, among the 96,798,000 blood components distributed, the European commission reported 111 transfusion‐transmitted infections in the European Union, including 73 bacterial (66%), 35 viral (32%), and 3 parasitic cases (<3%) . Bacterial contamination remains the highest residual infectious risk despite current preventive strategies …”

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Combining culture and microbead‐based immunoassay for the early and generic detection of bacteria in platelet concentrates

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BACKGROUND Despite current preventive strategies, bacterial contamination of platelets is the highest residual infectious risk in transfusion. Bacteria can grow from an initial concentration of 0.03–0.3 colony‐forming units (CFUs)/mL up to 108 to 109 CFUs/mL over the product shelf life. The aim of this study was to develop a cost‐effective approach for an early, rapid, sensitive, and generic detection of bacteria in platelet concentrates. STUDY DESIGN AND METHODS A large panel of bacteria involved in transfusion reactions, including clinical isolates and reference strains, was established. Sampling was performed 24 hours after platelet spiking. After an optimized culture step for increasing bacterial growth, a microbead‐based immunoassay allowed the generic detection of bacteria. Antibody production and immunoassay development took place exclusively with bacteria spiked in fresh platelet concentrates to improve the specificity of the test. RESULTS Antibodies for the generic detection of either gram‐negative or gram‐positive bacteria were selected for the microbead‐based immunoassay. Our approach, combining the improved culture step with the immunoassay, allowed sensitive detection of 1 to 10 CFUs/mL for gram‐negative and 1 to 102 CFUs/mL for gram‐positive species. CONCLUSION In this study, a new approach combining bacterial culture with immunoassay was developed for the generic and sensitive detection of bacteria in platelet concentrates. This efficient and easily automatable approach allows tested platelets to be used on Day 2 after collection and could represent an alternative strategy for reducing the risk of transfusion‐transmitted bacterial infections. This strategy could be adapted for the detection of bacteria in other cellular products.

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Prioritizing of bacterial infections transmitted through substances of human origin in Europe

Abstract: Six bacteria were defined as being of the highest priority in respect of the threat to the safety of SoHO and will be the subject of subsequent in-depth risk assessments to be conducted by ECDC to identify measures to mitigate the risk posed by these bacteria.

Cited by 16 publications

References 17 publications

Genotypic and phenotypic characteristics of Escherichia coli involved in transfusion‐transmitted bacterial infections: implications for preventive strategies

Genotypic and phenotypic characteristics of Escherichia coli involved in transfusion‐transmitted bacterial infections: implications for preventive strategies

Microbiological Screening of Platelet Concentrates in Europe

Combining culture and microbead‐based immunoassay for the early and generic detection of bacteria in platelet concentrates

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