The paper discusses the technology for the production of SPF eggs, i.e. specific pathogen free eggs, used in the manufacture of vaccines for veterinary use. Nowadays, such an egg is imported in the Russian Federation only from abroad, which indicates the dependence of the vaccine market on imports, as well as on the epidemiological situation in the supplying country. At the same time, the process of creating an enterprise for the production of SPF eggs is extremely laborious due to the increased requirements for the technological processes of keeping and growing poultry, as well as the high risks of accidental contamination of the livestock. Due to the fact that today one of the priority tasks is import substitution, the creation of Russia’s own production of SPF eggs is a strategically important task for the near future.